FastQCFastQC Report
Tue 31 May 2016
SRR212848_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212848_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322998
Sequences flagged as poor quality0
Sequence length56
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54241.679267363884606No Hit
TGATCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36611.1334435507340601No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT23670.7328218750580499No Hit
TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16150.5000030959944024No Hit
TGATCAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12400.38390330590282296No Hit
TGATCAGGGGCAGCAGAGCCCCAAAACTGGCCTGTAAAAGCAGCTGTTCTGAGCCC8860.2743051040563719No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGAAGCAGT8420.26068272868562653No Hit
TGATCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6710.20774122440386628No Hit
TGATCAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6490.2009300367184936No Hit
TGATCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA5750.17801967814042194No Hit
TGATCAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5390.1668740982916303No Hit
TGATCAGGGACAGCTACTTGCCCAGAGGCTTTGTAGCCTAGCTAGAGGGCCATGCC4490.1390101486696512No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4360.13498535594647645No Hit
TGATCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4260.13188936154403433No Hit
TGATCAGGGGAATAAATGCAGATGTAAACTGCAGTTCCTCCATACAACCAAACCCG4140.12817416826110378No Hit
TGATCAGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4100.12693577050012694No Hit
TGATCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4080.1263165716196385No Hit
TGATCAGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCAT3820.118266986173289No Hit
TGATCAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3420.10588300856352052No Hit
TGATCAGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3410.10557340912327631No Hit
TGATCAGGGATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGC3340.10340621304156682No Hit
TGATCAGGGATTTGATTGACATTTCACAGTGATTAATAAATTGTCCATCTAAATAA3330.10309661360132259No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGA204.08642E-450.22839446
CCGTCAA150.00829040350.20489542
CGGATTG451.3278623E-1050.13453740
CATCGTC150.00833649350.13453340
CCCAACG150.00834676150.11892738
AGTCGTG150.00834676150.11892738
TCGGAGC150.00837762150.0721633
CGTTAAG150.00839824150.04102731
AAAGCGT150.00839824150.04102731
GACGTTA150.00840856650.0254829
CGGGTGA150.00847590649.92463721
CCTCGAT252.1221449E-549.84734712
CGGTAGT950.049.84734712
AGATAGG355.4020347E-849.84734314
TTCGTAG150.00852797849.84734311
AAGCGTG150.00852797849.84734310
TGACGCA150.00852797849.84734315
TCGTAGG150.00852797849.84734312
GCTAACG150.00852797849.84734311
GACCTTA301.0677959E-649.8473439
Produced by FastQC (version 0.11.5)