FastQCFastQC Report
Tue 31 May 2016
SRR212847_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212847_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288307
Sequences flagged as poor quality0
Sequence length56
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46721.6204948197581053No Hit
TGATCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32471.1262300256323987No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT20460.7096601886183825No Hit
TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14280.49530535158702355No Hit
TGATCAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10890.37772235845817137No Hit
TGATCAGGGGCAGCAGAGCCCCAAAACTGGCCTGTAAAAGCAGCTGTTCTGAGCCC8280.2871938593235683No Hit
TGATCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5910.20498981987950343No Hit
TGATCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA5490.19042201542106157No Hit
TGATCAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4970.17238568609156213No Hit
TGATCAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4840.16787660375918725No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGAAGCAGT4700.16302066893970663No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4330.15018712691679356No Hit
TGATCAGGGACAGCTACTTGCCCAGAGGCTTTGTAGCCTAGCTAGAGGGCCATGCC4130.1432500771746784No Hit
TGATCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4020.13943469981651505No Hit
TGATCAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3990.13839414235519776No Hit
TGATCAGGGGAATAAATGCAGATGTAAACTGCAGTTCCTCCATACAACCAAACCCG3700.12833542022913078No Hit
TGATCAGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3650.12660115779360195No Hit
TGATCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3630.12590745281939045No Hit
TGATCAGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCAT3120.10821797597699674No Hit
TGATCAGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3020.10474945110593915No Hit
TGATCAGGGATGAATGTGGAGAGCAAACAAAAAGATGTAGGTTGCAAATGTTACCA2960.10266833618330459No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACTC150.00829867450.1908546
ACTCATA301.0180029E-650.1908546
CACGTCA204.1078578E-450.1733345
GTCGCCC150.00831587250.1645743
CGTTTAT150.00833309850.1383237
AGCACGC150.00833309850.1383238
ATGTACG150.0083503550.112134
ATCGGGT150.00837916250.06845532
CGAGTGC150.00840804850.02488728
ACTACGA204.1755344E-450.0074826
GTGAGCG150.00846604349.93797722
AAACGAT150.00852433449.8513712
GGATACG252.1205607E-549.8513688
CCGTAGA204.2404636E-449.85136811
GGGCGAT204.2404636E-449.8513687
CCGGTTA204.2404636E-449.85136818
ACCGTAG204.2404636E-449.85136810
AATAGAC204.2404636E-449.85136818
CAGGGAG45900.047.7878075
CAGGGAT51500.047.7217985
Produced by FastQC (version 0.11.5)