SRR212846_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212846_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	141071
Sequences flagged as poor quality	0
Sequence length	58
%GC	46

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCATCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2735	1.9387400670584316	No Hit
CCATCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1902	1.3482572605283865	No Hit
CCATCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1044	0.7400528811733099	No Hit
CCATCTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	755	0.5351914993159473	No Hit
CCATCTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	705	0.49974835366588455	No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	577	0.40901390080172395	No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA	400	0.2835451652005019	No Hit
CCATCTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT	356	0.25235519702844667	No Hit
CCATCTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	354	0.25093747120244414	No Hit
CCATCTGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT	339	0.24030452750742531	No Hit
CCATCTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	308	0.21832977720438645	No Hit
CCATCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT	302	0.2140765997263789	No Hit
CCATCTGGGGTGTGTATGTGTGTGTGTGTCAGTGTGGTATGTTTATGTGTGTGTCAGT	287	0.20344365603136008	No Hit
CCATCTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG	268	0.18997526068433626	No Hit
CCATCTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	261	0.18501322029332748	No Hit
CCATCTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	260	0.18430435738032622	No Hit
CCATCTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT	253	0.17934231698931744	No Hit
CCATCTGGGAGCAAGTCCCCTGTCCAGCCCCACATCAACCTCAGTGCAATATGCCCTC	252	0.17863345407631617	No Hit
CCATCTGGGATCGTGGCCAAAGGAGAAACCAGGAAGTTGGTACAGTGAATTTAAGAGA	247	0.1750891395113099	No Hit
CCATCTGGGATGTGGAGTCCCGTGTGCCATATGATATCTTAGCATGTTAATTGCACTT	246	0.17438027659830865	No Hit
CCATCTGGGATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGCTT	231	0.16374733290328983	No Hit
CCATCTGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	228	0.16162074416428607	No Hit
CCATCTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	225	0.1594941554252823	No Hit
CCATCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	224	0.15878529251228105	No Hit
CCATCTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	215	0.15240552629526977	No Hit
CCATCTGGGCGGGTGCGGATTGGTGCTAATTTAAACAAAAGGGCCTCTGGGGTCTTTT	199	0.14106371968724968	No Hit
CCATCTGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT	196	0.13893713094824592	No Hit
CCATCTGGGGATCTATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGAC	195	0.13822826803524466	No Hit
CCATCTGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGAC	193	0.13681054220924216	No Hit
CCATCTGGGTTTTTCCTGTGGGAGCAGCTGGGTAGAGAGGAGCGTGGCCTTCTCCTCT	190	0.1346839534702384	No Hit
CCATCTGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	186	0.13184850181823338	No Hit
CCATCTGGGGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGAC	180	0.12759532434022586	No Hit
CCATCTGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	179	0.1268864614272246	No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	167	0.11838010647120953	No Hit
CCATCTGGGAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAG	166	0.1176712435582083	No Hit
CCATCTGGGAGGTTCAGCGGGCTGCGCCCAGCTCTACCCCTAGGTGGCCATCTCCTCT	166	0.1176712435582083	No Hit
CCATCTGGGGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTT	161	0.11412692899320201	No Hit
CCATCTGGGATCTCAGAGGATCACAAGCTGAAGATTCCAGAAAACGCCAATGTGTTCT	148	0.1049117111241857	No Hit
CCATCTGGGGTGGCAGGGCACATTGGCCTTTTCTTACCCAGAACTCTTTTGTGTGGCT	143	0.10136739655917941	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCCGC	15	0.007194643	52.0227	45
TCACAAT	15	0.007194643	52.0227	41
AACATCG	20	3.4298882E-4	52.0227	49
GCGATCC	15	0.007194643	52.0227	52
CACAATT	15	0.007194643	52.0227	42
AATAGAG	40	1.8681021E-9	52.0227	50
ATTGCAC	25	1.6434862E-5	52.0227	50
ACGACGG	20	3.4298882E-4	52.0227	41
CGCCAAT	20	3.4298882E-4	52.0227	45
AGAACTC	35	3.848436E-8	52.004257	40
CAACGCC	15	0.007204745	52.004253	40
GCCGGCA	15	0.007204745	52.004253	31
GTAGGAC	15	0.007204745	52.004253	28
TACGGCT	15	0.007204745	52.004253	33
ACTAGTG	20	3.4359025E-4	52.004253	40
GCATTAC	15	0.007204745	52.004253	31
ACGGCTG	15	0.007204745	52.004253	34
TCTACCC	20	3.4359025E-4	52.004253	33
TTCTTAC	50	3.6379788E-12	52.004253	31
CGTAGGA	15	0.007204745	52.004253	27

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content