FastQCFastQC Report
Tue 31 May 2016
SRR212845_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212845_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150191
Sequences flagged as poor quality0
Sequence length58
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28581.902910294225353No Hit
CCATCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20111.3389617220738927No Hit
CCATCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11570.7703524179211804No Hit
CCATCTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG7840.5220019841401948No Hit
CCATCTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7500.49936414299125775No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA6560.43677717040302017No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA4120.274317369216531No Hit
CCATCTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3700.2463529772090205No Hit
CCATCTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3670.24435552063705548No Hit
CCATCTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3500.23303660006258695No Hit
CCATCTGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT3490.2323707812052653No Hit
CCATCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3330.22171767948811846No Hit
CCATCTGGGGTGTGTATGTGTGTGTGTGTCAGTGTGGTATGTTTATGTGTGTGTCAGT3270.21772276634418838No Hit
CCATCTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3010.20041147605382478No Hit
CCATCTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2890.19242164976596465No Hit
CCATCTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG2820.18776091776471293No Hit
CCATCTGGGATCGTGGCCAAAGGAGAAACCAGGAAGTTGGTACAGTGAATTTAAGAGA2720.18110272919149617No Hit
CCATCTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT2600.17311290290363604No Hit
CCATCTGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2540.16911798975970596No Hit
CCATCTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT2500.16645471433041928No Hit
CCATCTGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2490.16578889547309758No Hit
CCATCTGGGATGTGGAGTCCCGTGTGCCATATGATATCTTAGCATGTTAATTGCACTT2310.1538041560413074No Hit
CCATCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2310.1538041560413074No Hit
CCATCTGGGATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGCTT2210.1471459674680906No Hit
CCATCTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC2190.14581432975344727No Hit
CCATCTGGGGATCTATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGAC2160.14381687318148223No Hit
CCATCTGGGAGCAAGTCCCCTGTCCAGCCCCACATCAACCTCAGTGCAATATGCCCTC2110.14048777889487388No Hit
CCATCTGGGCGGGTGCGGATTGGTGCTAATTTAAACAAAAGGGCCTCTGGGGTCTTTT2060.1371586846082655No Hit
CCATCTGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGAC2060.1371586846082655No Hit
CCATCTGGGTTTTTCCTGTGGGAGCAGCTGGGTAGAGAGGAGCGTGGCCTTCTCCTCT1930.12850303946308367No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1920.127837220605762No Hit
CCATCTGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT1810.12051321317522355No Hit
CCATCTGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1810.12051321317522355No Hit
CCATCTGGGATCTCAGAGGATCACAAGCTGAAGATTCCAGAAAACGCCAATGTGTTCT1760.11718411888861516No Hit
CCATCTGGGAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAG1640.10919429260075504No Hit
CCATCTGGGGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGAC1620.10786265488611167No Hit
CCATCTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC1570.10453356059950329No Hit
CCATCTGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.10253610402753827No Hit
CCATCTGGGGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTT1540.10253610402753827No Hit
CCATCTGGGAGCCAGCACTAGCCTGGCCTTTGCCTAGCTACAACCACAGAGATGCAGA1530.10187028517021658No Hit
CCATCTGGGAGGTTCAGCGGGCTGCGCCCAGCTCTACCCCTAGGTGGCCATCTCCTCT1520.10120446631289493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACCG150.007163485552.0814450
TCAGCGA150.007163485552.0814450
TAGACGT150.00717294952.06406449
ATCAGCG150.00717294952.06406449
ATAGACG150.00718242252.04669648
GTCTCAA150.007191904452.02934342
TCGACCG150.007191904452.02934343
AAACGCC251.6427537E-552.0293442
AACGCCA251.6427537E-552.0293443
CAGCGTA150.007201395552.01236
AGCGTAG150.007201395552.01237
GTCGCTT150.007201395552.01230
CCTTACG307.935614E-752.01230
GTTCTTA150.007201395552.01235
TATGCTT150.007201395552.01235
GCCATAC150.007201395552.01229
ATACTCA150.007201395552.01239
ATATCCA150.007201395552.01230
TCGTGCC150.007201395552.01234
GCTTCGC150.007201395552.01234