SRR212845_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212845_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	150191
Sequences flagged as poor quality	0
Sequence length	58
%GC	46

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCATCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2858	1.902910294225353	No Hit
CCATCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2011	1.3389617220738927	No Hit
CCATCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1157	0.7703524179211804	No Hit
CCATCTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	784	0.5220019841401948	No Hit
CCATCTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	750	0.49936414299125775	No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	656	0.43677717040302017	No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA	412	0.274317369216531	No Hit
CCATCTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	370	0.2463529772090205	No Hit
CCATCTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT	367	0.24435552063705548	No Hit
CCATCTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	350	0.23303660006258695	No Hit
CCATCTGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT	349	0.2323707812052653	No Hit
CCATCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT	333	0.22171767948811846	No Hit
CCATCTGGGGTGTGTATGTGTGTGTGTGTCAGTGTGGTATGTTTATGTGTGTGTCAGT	327	0.21772276634418838	No Hit
CCATCTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	301	0.20041147605382478	No Hit
CCATCTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	289	0.19242164976596465	No Hit
CCATCTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG	282	0.18776091776471293	No Hit
CCATCTGGGATCGTGGCCAAAGGAGAAACCAGGAAGTTGGTACAGTGAATTTAAGAGA	272	0.18110272919149617	No Hit
CCATCTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT	260	0.17311290290363604	No Hit
CCATCTGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	254	0.16911798975970596	No Hit
CCATCTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	250	0.16645471433041928	No Hit
CCATCTGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	249	0.16578889547309758	No Hit
CCATCTGGGATGTGGAGTCCCGTGTGCCATATGATATCTTAGCATGTTAATTGCACTT	231	0.1538041560413074	No Hit
CCATCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	231	0.1538041560413074	No Hit
CCATCTGGGATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGCTT	221	0.1471459674680906	No Hit
CCATCTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	219	0.14581432975344727	No Hit
CCATCTGGGGATCTATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGAC	216	0.14381687318148223	No Hit
CCATCTGGGAGCAAGTCCCCTGTCCAGCCCCACATCAACCTCAGTGCAATATGCCCTC	211	0.14048777889487388	No Hit
CCATCTGGGCGGGTGCGGATTGGTGCTAATTTAAACAAAAGGGCCTCTGGGGTCTTTT	206	0.1371586846082655	No Hit
CCATCTGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGAC	206	0.1371586846082655	No Hit
CCATCTGGGTTTTTCCTGTGGGAGCAGCTGGGTAGAGAGGAGCGTGGCCTTCTCCTCT	193	0.12850303946308367	No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	192	0.127837220605762	No Hit
CCATCTGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT	181	0.12051321317522355	No Hit
CCATCTGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	181	0.12051321317522355	No Hit
CCATCTGGGATCTCAGAGGATCACAAGCTGAAGATTCCAGAAAACGCCAATGTGTTCT	176	0.11718411888861516	No Hit
CCATCTGGGAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAG	164	0.10919429260075504	No Hit
CCATCTGGGGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGAC	162	0.10786265488611167	No Hit
CCATCTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC	157	0.10453356059950329	No Hit
CCATCTGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	154	0.10253610402753827	No Hit
CCATCTGGGGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTT	154	0.10253610402753827	No Hit
CCATCTGGGAGCCAGCACTAGCCTGGCCTTTGCCTAGCTACAACCACAGAGATGCAGA	153	0.10187028517021658	No Hit
CCATCTGGGAGGTTCAGCGGGCTGCGCCCAGCTCTACCCCTAGGTGGCCATCTCCTCT	152	0.10120446631289493	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCACCG	15	0.0071634855	52.08144	50
TCAGCGA	15	0.0071634855	52.08144	50
TAGACGT	15	0.007172949	52.064064	49
ATCAGCG	15	0.007172949	52.064064	49
ATAGACG	15	0.007182422	52.046696	48
GTCTCAA	15	0.0071919044	52.029343	42
TCGACCG	15	0.0071919044	52.029343	43
AAACGCC	25	1.6427537E-5	52.02934	42
AACGCCA	25	1.6427537E-5	52.02934	43
CAGCGTA	15	0.0072013955	52.012	36
AGCGTAG	15	0.0072013955	52.012	37
GTCGCTT	15	0.0072013955	52.012	30
CCTTACG	30	7.935614E-7	52.012	30
GTTCTTA	15	0.0072013955	52.012	35
TATGCTT	15	0.0072013955	52.012	35
GCCATAC	15	0.0072013955	52.012	29
ATACTCA	15	0.0072013955	52.012	39
ATATCCA	15	0.0072013955	52.012	30
TCGTGCC	15	0.0072013955	52.012	34
GCTTCGC	15	0.0072013955	52.012	34

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content