FastQCFastQC Report
Tue 31 May 2016
SRR212844_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212844_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157296
Sequences flagged as poor quality0
Sequence length58
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27541.7508391821788223No Hit
CCATCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19521.2409724341369137No Hit
CCATCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10520.6688027667582138No Hit
CCATCTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG7910.5028735632183908No Hit
CCATCTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6940.4412063879564642No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA5900.37508900417048113No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3990.2536618858712237No Hit
CCATCTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3760.23903977214932356No Hit
CCATCTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3550.2256891465771539No Hit
CCATCTGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT3140.19962363950767978No Hit
CCATCTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG3110.19771640728308412No Hit
CCATCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2960.18818024616010579No Hit
CCATCTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2880.183094293561184No Hit
CCATCTGGGATCGTGGCCAAAGGAGAAACCAGGAAGTTGGTACAGTGAATTTAAGAGA2840.18055131726172313No Hit
CCATCTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2790.177372596887397No Hit
CCATCTGGGGTGTGTATGTGTGTGTGTGTCAGTGTGGTATGTTTATGTGTGTGTCAGT2730.17355813243820567No Hit
CCATCTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT2710.17228664428847523No Hit
CCATCTGGGAGCAAGTCCCCTGTCCAGCCCCACATCAACCTCAGTGCAATATGCCCTC2560.1627504831654969No Hit
CCATCTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2550.16211473909063168No Hit
CCATCTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT2340.148764113518462No Hit
CCATCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2320.14749262536873156No Hit
CCATCTGGGATGTGGAGTCCCGTGTGCCATATGATATCTTAGCATGTTAATTGCACTT2280.14494964906927066No Hit
CCATCTGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2210.14049944054521413No Hit
CCATCTGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2160.137320720170888No Hit
CCATCTGGGGATCTATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGAC2130.13541348794629235No Hit
CCATCTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC2030.1290560471976401No Hit
CCATCTGGGCGGGTGCGGATTGGTGCTAATTTAAACAAAAGGGCCTCTGGGGTCTTTT1990.12651307089817923No Hit
CCATCTGGGATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGCTT1950.12397009459871834No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1940.12333435052385311No Hit
CCATCTGGGTTTTTCCTGTGGGAGCAGCTGGGTAGAGAGGAGCGTGGCCTTCTCCTCT1840.1169769097752009No Hit
CCATCTGGGGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGAC1830.11634116570033567No Hit
CCATCTGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1790.11379818940087479No Hit
CCATCTGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGAC1770.11252670125114433No Hit
CCATCTGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT1690.10744074865222256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTG650.052.3336529
ATCTAAC203.3315533E-452.33365210
GGACGTG150.00702847552.3336528
AACCTAT150.00702847552.33365210
GATCTCG150.00702847552.3336529
TAGCGTG150.00717228852.0663938
TACGGGA150.00717228852.0663938
GTTAAGC203.4168828E-452.0663939
CCGTTGT307.8808625E-752.0663938
TCACGAA150.00719041752.03317342
GTCTACC150.00719041752.03317341
GTTATAC150.00719041752.03317340
TACGTTT150.00719041752.03317343
CGTTTGA353.8449798E-852.01658624
ACGCCGG1000.052.01658227
GCGCCCA203.433074E-452.01658224
ATATGAT401.87174E-952.01658229
ATGCCGC203.433074E-452.01658229
CGCATTC150.007199494652.0165826
ATTAAGC150.007199494652.0165851
Produced by FastQC (version 0.11.5)