FastQCFastQC Report
Tue 31 May 2016
SRR212843_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212843_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252555
Sequences flagged as poor quality0
Sequence length56
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43811.734671655678961No Hit
CCATCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29331.1613311951852072No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC24600.9740452574686701No Hit
CCATCTGGGGTGTGTATGTGTGTGTGTGTCAGTGTGGTATGTTTATGTGTGTGTCA14560.5765080873473105No Hit
CCATCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14220.5630456732196947No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC12010.4755399813901922No Hit
CCATCTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT11500.4553463601987686No Hit
CCATCTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11460.4537625467719903No Hit
CCATCTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG9100.36031755459206904No Hit
CCATCTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA5760.22806913345607888No Hit
CCATCTGGGATGTGGAGTCCCGTGTGCCATATGATATCTTAGCATGTTAATTGCAC5390.21341885925837936No Hit
CCATCTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4730.18728593771653698No Hit
CCATCTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4620.1829304507928966No Hit
CCATCTGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG4510.1785749638692562No Hit
CCATCTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4460.17659519708578328No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT4330.17144780344875374No Hit
CCATCTGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4280.16946803666528082No Hit
CCATCTGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4260.16867612995189168No Hit
CCATCTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3650.14452297519352222No Hit
CCATCTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3580.14175130169666014No Hit
CCATCTGGGGGTTTTGTGGGTGGGGAGGATGGAGATGGACATTAGTCATTAACATT3580.14175130169666014No Hit
CCATCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA3540.1401674882698818No Hit
CCATCTGGGATCGTGGCCAAAGGAGAAACCAGGAAGTTGGTACAGTGAATTTAAGA3500.1385836748431035No Hit
CCATCTGGGCGGGTGCGGATTGGTGCTAATTTAAACAAAAGGGCCTCTGGGGTCTT3490.1381877214864089No Hit
CCATCTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3490.1381877214864089No Hit
CCATCTGGGGATCTATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAG3450.13660390805963057No Hit
CCATCTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG3000.11878600700837441No Hit
CCATCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2810.11126289323117736No Hit
CCATCTGGGAGCAAGTCCCCTGTCCAGCCCCACATCAACCTCAGTGCAATATGCCC2680.1061154995941478No Hit
CCATCTGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2650.10492763952406406No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGG150.00826373250.2426250
AGGTCAT150.00831600450.1625546
GTCATAC150.00831600450.1625546
CCCAACG150.00831600450.1625543
TACGACT150.00831600450.1625544
CATACGA150.00831600450.1625542
ATACGAC150.00831600450.1625543
TCATACG150.00832910950.14257441
CTGTCGT204.1274272E-450.12261239
AGCGGCA150.00834879750.1126436
CTCGTGA204.1314834E-450.11263738
TTGACGG252.055241E-550.11263737
CGTACTT150.00835536850.1026733
GACCGTC150.00836194250.092732
ATACGTA150.0083685250.0827430
CTCTAAA150.0083685250.0827431
CCGTTAA252.0625126E-550.08273730
CTCCGAC150.0083948750.04292729
TACGGCC150.0083948750.04292729
AATACGT150.0083948750.04292729
Produced by FastQC (version 0.11.5)