SRR212842_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212842_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	237170
Sequences flagged as poor quality	0
Sequence length	56
%GC	46

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCATCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3895	1.642281907492516	No Hit
CCATCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2753	1.1607707551545303	No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	2166	0.9132689631909601	No Hit
CCATCTGGGGTGTGTATGTGTGTGTGTGTCAGTGTGGTATGTTTATGTGTGTGTCA	1484	0.6257115149470843	No Hit
CCATCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1198	0.5051229076190075	No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	1091	0.4600075894927689	No Hit
CCATCTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	997	0.42037357169962475	No Hit
CCATCTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	952	0.4013998397773748	No Hit
CCATCTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	920	0.38790740818821945	No Hit
CCATCTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA	595	0.25087489986085926	No Hit
CCATCTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	571	0.24075557616899268	No Hit
CCATCTGGGATGTGGAGTCCCGTGTGCCATATGATATCTTAGCATGTTAATTGCAC	461	0.19437534258127082	No Hit
CCATCTGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	451	0.19015895770965974	No Hit
CCATCTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	435	0.183412741915082	No Hit
CCATCTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	426	0.17961799553063204	No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	422	0.17793144158198762	No Hit
CCATCTGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	405	0.17076358730024876	No Hit
CCATCTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT	366	0.15431968630096554	No Hit
CCATCTGGGGATCTATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAG	365	0.15389804781380445	No Hit
CCATCTGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	353	0.14883838596787116	No Hit
CCATCTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	344	0.14504363958342117	No Hit
CCATCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	341	0.14377872412193785	No Hit
CCATCTGGGCGGGTGCGGATTGGTGCTAATTTAAACAAAAGGGCCTCTGGGGTCTT	336	0.1416705316861323	No Hit
CCATCTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	331	0.13956233925032677	No Hit
CCATCTGGGATCGTGGCCAAAGGAGAAACCAGGAAGTTGGTACAGTGAATTTAAGA	314	0.1323944849685879	No Hit
CCATCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	304	0.12817810009697686	No Hit
CCATCTGGGGGTTTTGTGGGTGGGGAGGATGGAGATGGACATTAGTCATTAACATT	303	0.12775646160981574	No Hit
CCATCTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	297	0.1252266306868491	No Hit
CCATCTGGGAGCAAGTCCCCTGTCCAGCCCCACATCAACCTCAGTGCAATATGCCC	278	0.11721549943078804	No Hit
CCATCTGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	261	0.11004764514904922	No Hit
CCATCTGGGGCGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTC	247	0.1041447063287937	No Hit
CCATCTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC	243	0.10245815238014926	No Hit
CCATCTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	240	0.10119323691866594	No Hit
CCATCTGGGATATAAAGGGTGTTCGTTTGACTTGTAGCTTACAAATGTAAATTTCC	238	0.1003499599443437	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCAGCG	25	2.0151576E-5	50.278084	49
CAAGCGC	25	2.0151576E-5	50.278084	50
AATAGAG	60	0.0	50.278084	50
GCTCGAG	15	0.008240084	50.278084	49
CGATCGA	40	2.5538611E-9	50.246014	45
CGCCAAT	15	0.008260898	50.246014	45
GATACGA	40	2.59206E-9	50.160686	41
AGATACG	40	2.59206E-9	50.160686	40
TTACGAC	20	4.111467E-4	50.160686	39
GACGCAG	15	0.008316594	50.160686	39
TCACGTC	15	0.008316594	50.160686	39
TGACGCA	15	0.008323575	50.150043	38
AACACAC	25	2.045798E-5	50.150043	37
GCCGACA	20	4.1157764E-4	50.15004	36
TATTACG	20	4.1157764E-4	50.15004	37
GCGATAC	40	2.6266207E-9	50.086266	32
CGCTGAT	15	0.008386606	50.054436	31
CGGGTAA	20	4.1590686E-4	50.043835	30
TACGAGG	15	0.008407695	50.022648	29
GGCATAG	15	0.008414733	50.012062	27

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content