FastQCFastQC Report
Tue 31 May 2016
SRR212842_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212842_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237170
Sequences flagged as poor quality0
Sequence length56
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38951.642281907492516No Hit
CCATCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27531.1607707551545303No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC21660.9132689631909601No Hit
CCATCTGGGGTGTGTATGTGTGTGTGTGTCAGTGTGGTATGTTTATGTGTGTGTCA14840.6257115149470843No Hit
CCATCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11980.5051229076190075No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC10910.4600075894927689No Hit
CCATCTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9970.42037357169962475No Hit
CCATCTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG9520.4013998397773748No Hit
CCATCTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT9200.38790740818821945No Hit
CCATCTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA5950.25087489986085926No Hit
CCATCTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG5710.24075557616899268No Hit
CCATCTGGGATGTGGAGTCCCGTGTGCCATATGATATCTTAGCATGTTAATTGCAC4610.19437534258127082No Hit
CCATCTGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG4510.19015895770965974No Hit
CCATCTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4350.183412741915082No Hit
CCATCTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4260.17961799553063204No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT4220.17793144158198762No Hit
CCATCTGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4050.17076358730024876No Hit
CCATCTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3660.15431968630096554No Hit
CCATCTGGGGATCTATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAG3650.15389804781380445No Hit
CCATCTGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3530.14883838596787116No Hit
CCATCTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3440.14504363958342117No Hit
CCATCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA3410.14377872412193785No Hit
CCATCTGGGCGGGTGCGGATTGGTGCTAATTTAAACAAAAGGGCCTCTGGGGTCTT3360.1416705316861323No Hit
CCATCTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3310.13956233925032677No Hit
CCATCTGGGATCGTGGCCAAAGGAGAAACCAGGAAGTTGGTACAGTGAATTTAAGA3140.1323944849685879No Hit
CCATCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3040.12817810009697686No Hit
CCATCTGGGGGTTTTGTGGGTGGGGAGGATGGAGATGGACATTAGTCATTAACATT3030.12775646160981574No Hit
CCATCTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2970.1252266306868491No Hit
CCATCTGGGAGCAAGTCCCCTGTCCAGCCCCACATCAACCTCAGTGCAATATGCCC2780.11721549943078804No Hit
CCATCTGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2610.11004764514904922No Hit
CCATCTGGGGCGTCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTC2470.1041447063287937No Hit
CCATCTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2430.10245815238014926No Hit
CCATCTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC2400.10119323691866594No Hit
CCATCTGGGATATAAAGGGTGTTCGTTTGACTTGTAGCTTACAAATGTAAATTTCC2380.1003499599443437No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGCG252.0151576E-550.27808449
CAAGCGC252.0151576E-550.27808450
AATAGAG600.050.27808450
GCTCGAG150.00824008450.27808449
CGATCGA402.5538611E-950.24601445
CGCCAAT150.00826089850.24601445
GATACGA402.59206E-950.16068641
AGATACG402.59206E-950.16068640
TTACGAC204.111467E-450.16068639
GACGCAG150.00831659450.16068639
TCACGTC150.00831659450.16068639
TGACGCA150.00832357550.15004338
AACACAC252.045798E-550.15004337
GCCGACA204.1157764E-450.1500436
TATTACG204.1157764E-450.1500437
GCGATAC402.6266207E-950.08626632
CGCTGAT150.00838660650.05443631
CGGGTAA204.1590686E-450.04383530
TACGAGG150.00840769550.02264829
GGCATAG150.00841473350.01206227