FastQCFastQC Report
Tue 31 May 2016
SRR212841_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212841_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183321
Sequences flagged as poor quality0
Sequence length58
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27481.4990099333955194No Hit
GAATTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19361.0560710447793762No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA18010.9824297270907315No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA11050.6027678225626087No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG9930.5416728034431407No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC8120.4429388886161433No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG7750.4227557126570333No Hit
GAATTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7420.40475450166647575No Hit
GAATTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT7050.38457132570736574No Hit
GAATTAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5800.3163849204401023No Hit
GAATTAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5520.3011111656602353No Hit
GAATTAGGGGGTGCTCCACTCTTCTGGTTCTTCAACATCTATGACCTTTTTATGGCTT5320.2902013408174732No Hit
GAATTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4910.2678361998898108No Hit
GAATTAGGGAGCCCAAGGCCAAGGACCCTTTCGCTCACCTGCCCAAGAGTACCTTTGT4160.22692435672945271No Hit
GAATTAGGGATCTTGGTTTTGTCCACTTCATCTTTATATGACATGTGACCCATTGTGA3990.2176510056131049No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCG3930.21437805816027625No Hit
GAATTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3920.21383256691813812No Hit
GAATTAGGGAGGTTACGGCCATTTGTTTAGGGGCATGAAGGAACGTTTTTAATATATG3890.21219609319172383No Hit
GAATTAGGGGGCTCGCACTCCGGCTCTGCTACCCCACAATCGGGTGCGGATTGGTGCT3690.20128626834896168No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3600.19637684716971868No Hit
GAATTAGGGAACATAGACTCATTTATTTAAATGTTTGGCCCATAGGTAATCACATTTT3590.1958313559275806No Hit
GAATTAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3380.18437603984268033No Hit
GAATTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA3250.17728465369488494No Hit
GAATTAGGGGACAAAAATTATGGCAATGCTCTGGAAATATACTTAACGTTAAGACATA3170.17292072375778006No Hit
GAATTAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3020.16473835512570845No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA2850.1554650040093606No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA2790.15219205655653198No Hit
GAATTAGGGAGATCACTGTATAAGTTGTGAGGGTCAGCTGGACGGATGAGGAACTCAG2520.1374637930188031No Hit
GAATTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA2480.13528182805025066No Hit
GAATTAGGGAGCTACAACCTGGCTGTACTAACTCCTGGATCTTTCCCTCTCTCCAACC2360.12873593314459336No Hit
GAATTAGGGGGGGGCTGGAGAAATGGCTCAGCGGTTAAGAGCACTGACTGTTCTTCCA2300.12546298569176473No Hit
GAATTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2200.12000807327038365No Hit
GAATTAGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCTGA2200.12000807327038365No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCTG2170.11837159954396932No Hit
GAATTAGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2100.11455316084900258No Hit
GAATTAGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2090.11400766960686447No Hit
GAATTAGGGGTTGCTGAATTCCTCACTGAACATTTCAATGAGGCTCCAGACCTTTATA2010.1096437396697596No Hit
GAATTAGGGGTCACTAACACTGTATGTGCAAATGTCCGCAATAAAACACTTTCCAACT2000.10909824842762149No Hit
GAATTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1990.10855275718548339No Hit
GAATTAGGGACCTGACACAGTTCCCTCACCAAAAGCACATCCAGCCAGTGGCCTGAAC1960.10691628345906906No Hit
GAATTAGGGATCCTATGGGTGTTCCAAGGCACCCAATGCTCCACCCTTTAATACAGTT1920.10473431849051663No Hit
GAATTAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC1880.1025523535219642No Hit
GAATTAGGGGGGCTGGAGAGATGGCTCAATGGTTAAGAGCACCGACTGCTCTTCCAAA1860.10146137103768799No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTAAT150.007162109552.088652
TTTACGG203.4112093E-452.08859651
CAGCTCG203.4112093E-452.08859651
AGCTCGA203.4112093E-452.08859652
TATTCGT150.0071698652.0743650
CGTTTAG203.4158188E-452.0743650
TTTTACG150.0071698652.0743650
CATATAG150.00717761652.06012748
CTCGATC150.00717761652.06012748
ATATTCG150.00717761652.06012749
ATATCTC150.007185378652.04590243
GTACTAG150.007185378652.04590243
TTAACGT459.094947E-1152.04590243
ATCCGTG251.6490794E-552.0032834
AACTGAT251.6490794E-552.0032834
TAACTGA251.6490794E-552.0032833
TCACGGC150.00720870452.00327733
TTTAGGG600.052.00327726
TAGAGCG459.276846E-1152.00327734
CGTTGGA150.00720870452.00327728
Produced by FastQC (version 0.11.5)