FastQCFastQC Report
Tue 31 May 2016
SRR212840_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212840_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183707
Sequences flagged as poor quality0
Sequence length58
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27621.5034810867305002No Hit
GAATTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18651.0152035578393857No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA17290.9411726281524384No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA10290.5601310782931516No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG9280.5051522260991688No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC7610.4142465992041675No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG7480.4071701132782093No Hit
GAATTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7430.4044483879220716No Hit
GAATTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT7280.3962832118536583No Hit
GAATTAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5760.3135427610270703No Hit
GAATTAGGGGGTGCTCCACTCTTCTGGTTCTTCAACATCTATGACCTTTTTATGGCTT5300.2885028877506029No Hit
GAATTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4980.27108384547132114No Hit
GAATTAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4810.26182997926045276No Hit
GAATTAGGGAGGTTACGGCCATTTGTTTAGGGGCATGAAGGAACGTTTTTAATATATG4560.24822135247976398No Hit
GAATTAGGGAGCCCAAGGCCAAGGACCCTTTCGCTCACCTGCCCAAGAGTACCTTTGT4030.2193710637047037No Hit
GAATTAGGGATCTTGGTTTTGTCCACTTCATCTTTATATGACATGTGACCCATTGTGA3940.21447195806365574No Hit
GAATTAGGGGGCTCGCACTCCGGCTCTGCTACCCCACAATCGGGTGCGGATTGGTGCT3740.20358505663910464No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3640.19814160592682914No Hit
GAATTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3510.19106512000087095No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCG3460.18834339464473318No Hit
GAATTAGGGAACATAGACTCATTTATTTAAATGTTTGGCCCATAGGTAATCACATTTT3300.17963387350509236No Hit
GAATTAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3220.17527911293527193No Hit
GAATTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2980.16221483122581065No Hit
GAATTAGGGGACAAAAATTATGGCAATGCTCTGGAAATATACTTAACGTTAAGACATA2920.15894876079844536No Hit
GAATTAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2880.15677138051353515No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA2730.14860620444512185No Hit
GAATTAGGGAGATCACTGTATAAGTTGTGAGGGTCAGCTGGACGGATGAGGAACTCAG2590.14098537344793613No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA2510.1366306128781157No Hit
GAATTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA2480.13499757766443304No Hit
GAATTAGGGGGGGGCTGGAGAAATGGCTCAGCGGTTAAGAGCACTGACTGTTCTTCCA2370.12900978188092996No Hit
GAATTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC2360.1284654368097024No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCTG2360.1284654368097024No Hit
GAATTAGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2230.12138895088374421No Hit
GAATTAGGGAGCTACAACCTGGCTGTACTAACTCCTGGATCTTTCCCTCTCTCCAACC2200.11975591567006158No Hit
GAATTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2150.1170341903139238No Hit
GAATTAGGGACCTGACACAGTTCCCTCACCAAAAGCACATCCAGCCAGTGGCCTGAAC2140.11648984524269626No Hit
GAATTAGGGGTCACTAACACTGTATGTGCAAATGTCCGCAATAAAACACTTTCCAACT2130.1159455001714687No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCTGA2050.11159073960164828No Hit
GAATTAGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2010.10941335931673807No Hit
GAATTAGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCTGA2000.10886901424551053No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAAAGCACCCGACTGCTCTTCCGA1980.10778032410305541No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAAAT150.00717264852.06928624
CACAGCG150.00717264852.06928651
CACCGTC150.00717264852.06928624
CGGTTAC353.81915E-852.06928351
TCGAACA353.81915E-852.06928325
TACGTTG203.4174824E-452.06928351
CTACGTT203.422089E-452.05508850
CTGGTCG150.0071803952.05508450
ACTACGT203.4267004E-452.04089449
CTAGAGG150.007195893752.0267147
GGTAATC550.052.0267145
CTATATC150.007195893752.0267141
TTATGCA203.4313166E-452.0267143
TAACGTG203.4313166E-452.0267144
TATGACC850.052.0267140
GTAGACC150.007195893752.0267145
TCCCGGT251.6446995E-552.0267146
CTCGATC150.007195893752.0267148
TTACCGT150.007195893752.0267140
GATTATG203.4313166E-452.0267141
Produced by FastQC (version 0.11.5)