FastQCFastQC Report
Tue 31 May 2016
SRR212839_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212839_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182731
Sequences flagged as poor quality0
Sequence length58
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24561.344052186000186No Hit
GAATTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16590.9078919285726011No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA15720.8602809594431158No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA9860.5395909834675015No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG8770.4799404589259622No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC7210.3945690660041263No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG6990.3825295105920725No Hit
GAATTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6760.36994270266128904No Hit
GAATTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT6510.35626138969304605No Hit
GAATTAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4990.273079006846129No Hit
GAATTAGGGGGTGCTCCACTCTTCTGGTTCTTCAACATCTATGACCTTTTTATGGCTT4950.2708899967712101No Hit
GAATTAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4870.2665119766213724No Hit
GAATTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4720.25830318884042663No Hit
GAATTAGGGGGCTCGCACTCCGGCTCTGCTACCCCACAATCGGGTGCGGATTGGTGCT3850.21069221971094124No Hit
GAATTAGGGAGGTTACGGCCATTTGTTTAGGGGCATGAAGGAACGTTTTTAATATATG3790.2074087045985629No Hit
GAATTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3570.19536914918650913No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3440.1882548664430228No Hit
GAATTAGGGATCTTGGTTTTGTCCACTTCATCTTTATATGACATGTGACCCATTGTGA3410.18661310888683366No Hit
GAATTAGGGAGCCCAAGGCCAAGGACCCTTTCGCTCACCTGCCCAAGAGTACCTTTGT3370.18442409881191477No Hit
GAATTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA3020.16527026065637468No Hit
GAATTAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2840.15541971531923976No Hit
GAATTAGGGAACATAGACTCATTTATTTAAATGTTTGGCCCATAGGTAATCACATTTT2830.15487246280051004No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA2820.15432521028178034No Hit
GAATTAGGGGACAAAAATTATGGCAATGCTCTGGAAATATACTTAACGTTAAGACATA2630.1439274124259157No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCG2590.1417384023509968No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA2480.13571862464496992No Hit
GAATTAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2470.1351713721262402No Hit
GAATTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA2430.13298236205132136No Hit
GAATTAGGGAGATCACTGTATAAGTTGTGAGGGTCAGCTGGACGGATGAGGAACTCAG2340.1280570893827539No Hit
GAATTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2280.12477357427037557No Hit
GAATTAGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCTGA2050.11218676633959208No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCTG2040.11163951382086236No Hit
GAATTAGGGGGGGGCTGGAGAAATGGCTCAGCGGTTAAGAGCACTGACTGTTCTTCCA2010.10999775626467322No Hit
GAATTAGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910.10452523107737602No Hit
GAATTAGGGACCTGACACAGTTCCCTCACCAAAAGCACATCCAGCCAGTGGCCTGAAC1870.10233622100245716No Hit
GAATTAGGGAGCTACAACCTGGCTGTACTAACTCCTGGATCTTTCCCTCTCTCCAACC1860.10178896848372744No Hit
GAATTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1840.10069446344626802No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAAAGCACCCGACTGCTCTTCCGA1830.1001472109275383No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCTGA1830.1001472109275383No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCT150.007008717452.3743449
AGTCATA203.3202223E-452.37434410
CAACGAT150.007008717452.37434410
GAGTCAT458.54925E-1152.3743449
GGGTCGA203.3202223E-452.3743448
ATAATCA150.007008717452.37434410
GATCCAC150.007008717452.3743449
GGATCTA251.5810485E-552.3743448
GGATAAT150.007008717452.3743448
GGTGCAA353.646892E-852.3743449
AATATAC401.8662831E-952.0434136
CGTTTAC150.0071866852.04340436
CCGCAAT150.0071866852.04340436
GTCGCTA203.4351196E-452.0148333
CTTACCC459.094947E-1152.01482833
ACCGGCC150.007202312452.01482433
GTGCACG150.00721013852.00054644
AACCGGT251.6495558E-552.00054644
AGTCCGC150.00721013852.00054652
CCCGTAG150.00721013852.00054644
Produced by FastQC (version 0.11.5)