FastQCFastQC Report
Tue 31 May 2016
SRR212838_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212838_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174237
Sequences flagged as poor quality0
Sequence length56
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26351.5123079483691753No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC19521.1203131367046035No Hit
GAATTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18111.0393888783668224No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC10180.584261666580577No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC9590.5503997428789522No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC9010.5171117500875244No Hit
GAATTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT7200.4132302553418619No Hit
GAATTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7190.4126563244316649No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT6640.38109012437082823No Hit
GAATTAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5930.34034102974683905No Hit
GAATTAGGGGGTGCTCCACTCTTCTGGTTCTTCAACATCTATGACCTTTTTATGGC4620.26515608051102807No Hit
GAATTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4040.2318680877196003No Hit
GAATTAGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCT3990.22899843316861518No Hit
GAATTAGGGAGGTTACGGCCATTTGTTTAGGGGCATGAAGGAACGTTTTTAATATA3830.21981553860546268No Hit
GAATTAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3650.20948478222191613No Hit
GAATTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3590.20604119676073396No Hit
GAATTAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC3580.20546726585053693No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTC3480.19972795674856658No Hit
GAATTAGGGACCTGACACAGTTCCCTCACCAAAAGCACATCCAGCCAGTGGCCTGA3400.19513650946699038No Hit
GAATTAGGGATCTTGGTTTTGTCCACTTCATCTTTATATGACATGTGACCCATTGT3320.19054506218541412No Hit
GAATTAGGGAACATAGACTCATTTATTTAAATGTTTGGCCCATAGGTAATCACATT3270.18767540763442897No Hit
GAATTAGGGGACAAAAATTATGGCAATGCTCTGGAAATATACTTAACGTTAAGACA3100.17791858216107945No Hit
GAATTAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2990.1716053421489121No Hit
GAATTAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2950.16930961850812398No Hit
GAATTAGGGAGCCCAAGGCCAAGGACCCTTTCGCTCACCTGCCCAAGAGTACCTTT2860.16414424031635072No Hit
GAATTAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT2810.16127458576536557No Hit
GAATTAGGGAGATCACTGTATAAGTTGTGAGGGTCAGCTGGACGGATGAGGAACTC2440.14003914208807544No Hit
GAATTAGGGGGCTCGCACTCCGGCTCTGCTACCCCACAATCGGGTGCGGATTGGTG2390.1371694875370903No Hit
GAATTAGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2360.1354476948064992No Hit
GAATTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2230.1279865929739378No Hit
GAATTAGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2140.12282121478216451No Hit
GAATTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2100.1205254911413764No Hit
GAATTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC2030.11650797476999719No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTC1970.113064389308815No Hit
GAATTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1920.11019473475782984No Hit
GAATTAGGGATTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCCT1890.10847294202723876No Hit
GAATTAGGGGGGGGCTGGAGAAATGGCTCAGCGGTTAAGAGCACTGACTGTTCTTC1830.10502935656605658No Hit
GAATTAGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1750.10043790928448033No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACTG150.008219033550.30492850
TGTATGC150.008219033550.30492850
CTTAAAT150.0082378950.27578449
GTTAGTT204.0621916E-450.27578448
TGATTAA204.0621916E-450.27578449
ACTCCGT301.0038548E-650.27578449
GCATATA150.0082378950.27578448
GCGAAGT150.00826623550.2321346
GTACTCC150.00826623550.2321346
CTTACTC301.009892E-650.2321346
CGTTAAG402.5538611E-950.23212447
TCGCCAA204.085482E-450.21759442
CTTAACG402.559318E-950.21759442
AACGTTA402.559318E-950.21759445
GGTAATC355.077709E-850.21759445
ATAGGTA355.077709E-850.21759442
TAGGTAA355.077709E-850.21759443
CCGTGTC204.085482E-450.21759443
CATAGGT355.077709E-850.21759441
ACGTGTT204.085482E-450.21759442
Produced by FastQC (version 0.11.5)