FastQCFastQC Report
Tue 31 May 2016
SRR212837_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212837_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156198
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22481.4391989654156903No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC16581.0614732583003623No Hit
GAATTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15560.9961715258838142No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC8900.5697896259875286No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC8880.568509199861714No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC8610.5512234471632159No Hit
GAATTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT6480.4148580647639535No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT6170.39501145981382607No Hit
GAATTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6100.3905299683734747No Hit
GAATTAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4550.2912969436228377No Hit
GAATTAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC4490.2874556652453937No Hit
GAATTAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4370.27977310849050563No Hit
GAATTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3660.23431798102408483No Hit
GAATTAGGGGGTGCTCCACTCTTCTGGTTCTTCAACATCTATGACCTTTTTATGGC3650.23367776796117748No Hit
GAATTAGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCT3470.22215393282884544No Hit
GAATTAGGGATCTTGGTTTTGTCCACTTCATCTTTATATGACATGTGACCCATTGT3170.20294754094162537No Hit
GAATTAGGGAGGTTACGGCCATTTGTTTAGGGGCATGAAGGAACGTTTTTAATATA3160.20230732787871802No Hit
GAATTAGGGAGCCCAAGGCCAAGGACCCTTTCGCTCACCTGCCCAAGAGTACCTTT3130.200386688689996No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTC2950.188862853557664No Hit
GAATTAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT2890.18502157518021997No Hit
GAATTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2770.17733901842533195No Hit
GAATTAGGGAACATAGACTCATTTATTTAAATGTTTGGCCCATAGGTAATCACATT2680.17157710085916594No Hit
GAATTAGGGACCTGACACAGTTCCCTCACCAAAAGCACATCCAGCCAGTGGCCTGA2640.1690162486075366No Hit
GAATTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2600.16645539635590725No Hit
GAATTAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2490.15941305266392655No Hit
GAATTAGGGGGCTCGCACTCCGGCTCTGCTACCCCACAATCGGGTGCGGATTGGTG2440.15621198734938987No Hit
GAATTAGGGGACAAAAATTATGGCAATGCTCTGGAAATATACTTAACGTTAAGACA2390.1530109220348532No Hit
GAATTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2380.15237070897194588No Hit
GAATTAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2280.14596857834287252No Hit
GAATTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC2150.13764580852507713No Hit
GAATTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2060.13188389095891112No Hit
GAATTAGGGAGATCACTGTATAAGTTGTGAGGGTCAGCTGGACGGATGAGGAACTC2000.1280426125814671No Hit
GAATTAGGGGTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT1920.12292090807820844No Hit
GAATTAGGGGGGGGCTGGAGAAATGGCTCAGCGGTTAAGAGCACTGACTGTTCTTC1850.11843941663785706No Hit
GAATTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1750.11203728600878372No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCT1690.10819600763133971No Hit
GAATTAGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC1650.10563515537971037No Hit
GAATTAGGGATTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCCT1630.10435472925389569No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTC1580.10115366393935901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACAC301.0152125E-650.18897241
GTACACC301.0152125E-650.18897242
ACGTTCA150.00829276850.18897249
CCAATAA150.00829276850.18897247
CGGATGA301.0152125E-650.18897243
TATTCGT150.00829276850.18897250
ACGGATG301.0152125E-650.18897242
ACGTGAA301.0152125E-650.18897246
GACGGAT301.0152125E-650.18897241
CTCGATC301.0152125E-650.18897248
GTATATG150.00829276850.18897244
ACCAATA150.00829276850.18897246
GCATATA150.00829276850.18897248
GGTAATC402.570232E-950.1889745
CGTGAAA252.0331763E-550.1889747
ACTCCGT204.095676E-450.1889749
AAGTCAC204.095676E-450.1889741
GATAAGT150.00830334850.17279438
TAAGTCA150.00830334850.17279440
GGACGGA301.0174754E-650.17279440
Produced by FastQC (version 0.11.5)