##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212837_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 156198 Sequences flagged as poor quality 0 Sequence length 56 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.30599623554719 39.0 38.0 40.0 35.0 40.0 2 36.974769203190824 39.0 36.0 40.0 31.0 40.0 3 37.899781047132485 39.0 38.0 40.0 34.0 40.0 4 38.100103714516194 39.0 38.0 40.0 35.0 40.0 5 38.26557958488585 39.0 38.0 40.0 35.0 40.0 6 38.02571735873699 39.0 38.0 40.0 35.0 40.0 7 38.509385523502225 39.0 38.0 40.0 36.0 40.0 8 38.516831201423834 39.0 38.0 40.0 36.0 40.0 9 38.48977579738537 39.0 38.0 40.0 36.0 40.0 10 37.477534923622585 39.0 37.0 40.0 33.0 40.0 11 37.51298352091576 39.0 38.0 40.0 33.0 40.0 12 37.55759996926977 39.0 38.0 40.0 33.0 40.0 13 37.59109591672109 39.0 38.0 40.0 33.0 40.0 14 37.574527202653044 39.0 38.0 40.0 33.0 40.0 15 37.566793428853124 39.0 38.0 40.0 33.0 40.0 16 37.47540941625373 39.0 38.0 40.0 33.0 40.0 17 37.18068093061371 39.0 36.0 40.0 33.0 40.0 18 37.09979641224599 39.0 36.0 40.0 33.0 40.0 19 37.14064840779011 39.0 36.0 40.0 33.0 40.0 20 37.017381784657935 39.0 36.0 40.0 32.0 40.0 21 36.84297494206072 39.0 36.0 40.0 32.0 40.0 22 36.835939000499366 39.0 36.0 40.0 32.0 40.0 23 36.54859217147467 39.0 36.0 40.0 31.0 40.0 24 36.506735041421784 39.0 36.0 40.0 31.0 40.0 25 36.45538995377662 39.0 36.0 40.0 31.0 40.0 26 35.99336099053765 38.0 35.0 40.0 30.0 40.0 27 35.871176327481784 38.0 35.0 40.0 30.0 40.0 28 35.550007042343694 38.0 35.0 40.0 29.0 40.0 29 35.51700405895082 38.0 35.0 40.0 29.0 40.0 30 35.085135533105415 38.0 34.0 40.0 28.0 40.0 31 34.67833134867284 38.0 34.0 39.0 27.0 40.0 32 34.47375766655143 38.0 33.0 39.0 26.0 40.0 33 34.40324459980281 38.0 33.0 39.0 26.0 40.0 34 34.47114559725477 38.0 33.0 40.0 26.0 40.0 35 34.09911778639931 38.0 33.0 39.0 25.0 40.0 36 34.197915466267176 38.0 34.0 40.0 25.0 40.0 37 33.98532631659816 38.0 33.0 40.0 24.0 40.0 38 33.75822993892368 38.0 33.0 39.0 23.0 40.0 39 33.62871483629752 38.0 33.0 39.0 23.0 40.0 40 33.35412105148593 37.0 33.0 39.0 22.0 40.0 41 32.85724529123292 37.0 33.0 39.0 17.0 40.0 42 32.645578048374496 37.0 32.0 39.0 15.0 40.0 43 32.49485908910486 36.0 32.0 39.0 14.0 40.0 44 32.37599713184548 36.0 32.0 39.0 11.0 40.0 45 31.917630187326342 36.0 31.0 39.0 2.0 40.0 46 31.17568726872303 36.0 31.0 39.0 2.0 40.0 47 30.84634886490224 36.0 30.0 39.0 2.0 40.0 48 30.650366842085045 35.0 30.0 39.0 2.0 40.0 49 30.468520723696848 35.0 30.0 39.0 2.0 40.0 50 30.024628996530044 35.0 29.0 39.0 2.0 40.0 51 29.297532618855556 35.0 29.0 38.0 2.0 40.0 52 29.084674579700124 35.0 29.0 38.0 2.0 40.0 53 28.83259068618036 35.0 28.0 38.0 2.0 40.0 54 28.508630072087993 35.0 27.0 38.0 2.0 40.0 55 28.070993226545795 35.0 26.0 38.0 2.0 40.0 56 15.912630123305036 20.0 2.0 23.0 2.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.5443504342728573 1 2 -0.3320498173380031 1 3 -0.8461133176816773 1 4 -0.9453543247152609 1 5 -0.8361342167188823 1 6 -0.5150750006602607 1 7 -0.3523899026335471 1 8 -0.5065491493640266 1 9 -0.6829533669659256 1 10 -0.5885321648026576 1 11 -0.5068836743624772 1 12 -0.9046984105643432 1 13 -0.46246646582651607 1 14 -0.8531019786772802 1 15 -0.8948954031180918 1 16 -0.36135588187329404 1 17 -0.17155447534799606 1 18 -0.481469579590005 1 19 -0.09093127392326039 1 20 -0.40231448183434537 1 21 0.3475375403003653 1 22 -0.3135679161352769 1 23 -0.16114244097895636 1 24 -0.4069966362773272 1 25 -0.288754885816104 1 26 -0.1739529876033572 1 27 0.13983278455936698 1 28 -0.3973225158873035 1 29 0.25127357160609876 1 30 -0.11742234521887696 1 31 0.9251924887529412 1 32 0.9969915142019232 1 33 -0.26930839577309484 1 34 0.4438625677552537 1 35 0.3664600869812418 1 36 -0.051968601378334256 1 37 -0.07830231038056468 1 38 -0.5442068961845763 1 39 -0.6898117038349412 1 40 -0.7204848476817602 1 41 0.12222577102545529 1 42 -0.4396022116947833 1 43 -0.40310613831070086 1 44 0.17305215639701288 1 45 -0.5118403656031134 1 46 0.14680391373581614 1 47 0.1476642869898228 1 48 0.028152597258781498 1 49 -0.5640284193282739 1 50 0.0018395422282750928 1 51 -0.3375583097302304 1 52 0.3848560004553967 1 53 0.1116684294385486 1 54 0.1950345833965237 1 55 1.0268534894960872 1 56 0.8582354094195459 2 1 -0.3644417584737738 2 2 -1.0560680821781858 2 3 -0.36793980169994 2 4 -0.7962219046239412 2 5 -0.5602438057599812 2 6 -1.1485909823954188 2 7 -0.796590815875561 2 8 -0.546731797765851 2 9 -0.5180218601166118 2 10 -0.9396737173140721 2 11 -1.8077969163716077 2 12 -1.1753833420711928 2 13 -1.5335166941370204 2 14 -0.9856133942023959 2 15 -1.2207401519765426 2 16 -1.1840956079006872 2 17 -1.6045225118776756 2 18 -1.037907935754383 2 19 -0.9390591278045406 2 20 -0.7571090023822862 2 21 -1.4402250167772621 2 22 -0.958042801980028 2 23 -1.133471208102243 2 24 -0.8514715221220754 2 25 -0.7954215524827717 2 26 -0.5979712524435428 2 27 -0.428934338728304 2 28 -0.33659192228000023 2 29 -1.3775848758824836 2 30 -0.2512122995567765 2 31 -0.06814084458039105 2 32 -0.02675277803552234 2 33 -0.802459080704601 2 34 0.1284287778009201 2 35 -0.37801479886351075 2 36 0.5139674716810276 2 37 0.3605104750075654 2 38 0.7260214143177066 2 39 0.48918372995501613 2 40 0.033396430857052906 2 41 0.5269746294729458 2 42 0.7920872860221166 2 43 0.5992682909130451 2 44 0.5889425673559145 2 45 1.3340043832553334 2 46 0.73648427903262 2 47 0.019445108907632402 2 48 0.41518456072910226 2 49 0.0971587952835975 2 50 0.6934377157442562 2 51 0.9827156628725078 2 52 1.2045820278526556 2 53 0.7661889773837522 2 54 0.008550565131685062 2 55 1.1640224392677752 2 56 0.8742171445793652 3 1 -0.5902920986098223 3 2 -0.14600005496730262 3 3 -0.7309329989788509 3 4 -0.41581374135863314 3 5 -0.5395635336511404 3 6 -0.7089991456607265 3 7 -0.3466588430864448 3 8 -0.309316831779455 3 9 -0.18509669004858154 3 10 -1.4927349418038744 3 11 -1.1533751476641285 3 12 -0.752662253635819 3 13 -0.8853534288308964 3 14 -1.028062373794235 3 15 -0.5120326689833448 3 16 -0.45729288681225455 3 17 -1.076223192149783 3 18 -0.6489283439176532 3 19 -0.553889059777326 3 20 -1.0183334921782077 3 21 -0.6731501868452838 3 22 -0.39804280198002573 3 23 -0.5299337931362587 3 24 -0.76698172620371 3 25 -0.47242835520385995 3 26 -0.2668147898585076 3 27 -0.1855329781840851 3 28 0.07225161513496658 3 29 -0.41255086227704396 3 30 0.14266525146363307 3 31 -0.13943336158719433 3 32 0.007941099515498706 3 33 0.14570418460152013 3 34 0.13890496827711019 3 35 -0.17379711178868007 3 36 -0.630386269815574 3 37 -0.38982966104686056 3 38 -1.1028221230972548 3 39 -0.5727210319497473 3 40 -1.4244266983946474 3 41 -0.9241818331120868 3 42 -1.3107698568350301 3 43 -0.9671262669100855 3 44 -1.149424779582862 3 45 -1.010349358241264 3 46 -0.5752164012394871 3 47 -1.4020514897318215 3 48 -0.34250251410083266 3 49 -0.5144058305667443 3 50 -1.06492963119452 3 51 -0.9276244731819112 3 52 -1.3720166116031258 3 53 -0.34891306343257256 3 54 -0.7947147409907664 3 55 -0.5776102137934487 3 56 -0.4455107465770993 4 1 0.026549232517218968 4 2 -0.6313833974935008 4 3 -0.3808226845828244 4 4 0.005219536817499204 4 5 -0.3551086706248441 4 6 -0.2584108022152378 4 7 0.1454812561965113 4 8 0.07443937340531903 4 9 0.0425186804239317 4 10 0.22338934574898417 4 11 -0.2505897091644016 4 12 -0.2040319907198409 4 13 0.07585267523234762 4 14 0.08312534453634157 4 15 0.19673732550094059 4 16 -0.3222037160087936 4 17 -0.6105585479137119 4 18 -0.5911511789976274 4 19 -0.2605906593360672 4 20 -0.835487380760668 4 21 -0.40301780957005207 4 22 0.0456508917136631 4 23 -0.10320093783197137 4 24 -0.25723728788784683 4 25 -0.3833494804107005 4 26 0.31725397278168543 4 27 0.1489034991095366 4 28 0.03548014979207181 4 29 0.5812439529463447 4 30 0.28437328602880996 4 31 -0.08156426800381666 4 32 0.39712109583835087 4 33 0.1361895679440437 4 34 -0.026796447424310088 4 35 0.46892213807343097 4 36 -0.39017667246312016 4 37 -0.8187688042717127 4 38 -0.2755101172138197 4 39 -0.09910455833327347 4 40 -0.11209906463844277 4 41 0.4962539087522231 4 42 -0.02827307433824444 4 43 0.4899890116337673 4 44 0.3770506754640195 4 45 0.6667971760481279 4 46 0.22603382858217103 4 47 1.1975532170157415 4 48 1.09590528144982 4 49 1.0075191556439584 4 50 1.2340683463748867 4 51 1.3483913385481863 4 52 1.0029604062310362 4 53 1.0345673557621318 4 54 1.485667682248799 4 55 1.0828512680966043 4 56 1.1467396671018868 5 1 -0.028809574565727303 5 2 -0.19652785229312997 5 3 0.1750487040471853 5 4 0.10400798043352921 5 5 0.07010102182622546 5 6 0.24704120151262288 5 7 0.062489643894558355 5 8 0.14867050108472313 5 9 0.23301262264201483 5 10 0.4109009953296052 5 11 -0.23768197384287504 5 12 0.14116838206673776 5 13 0.15246031735723165 5 14 -0.013659371213890381 5 15 -0.5382114163443532 5 16 0.3290078403751764 5 17 0.21455794789243754 5 18 0.23312654700423252 5 19 0.21289489518397176 5 20 -0.20538486445125415 5 21 0.7271313050618247 5 22 0.24103765779008768 5 23 -0.18036775982638176 5 24 -0.007563476145065806 5 25 0.08365890728734371 5 26 0.6236379429587586 5 27 0.021410488857902976 5 28 0.19559198576597936 5 29 0.20011627354280392 5 30 0.7637302291788544 5 31 0.059675247373633056 5 32 0.13071848633229166 5 33 0.606506436536776 5 34 0.049578203088273654 5 35 -0.2237619253002876 5 36 -0.2655727582040299 5 37 -0.41765044453266853 5 38 -0.7916797351075786 5 39 -0.6567932815392368 5 40 -0.22062655764869277 5 41 -0.8169334165040638 5 42 -1.1150391507594968 5 43 -0.9347546975927017 5 44 -0.4931263981613263 5 45 -0.420248490307884 5 46 -0.2867341117719775 5 47 -0.7088307531613331 5 48 -0.47883842777664753 5 49 -0.7453699403485885 5 50 -0.15805653712930834 5 51 -0.4290084750585237 5 52 -0.2547283169749299 5 53 -0.6972592984783148 5 54 -0.7670816187763627 5 55 -0.5948281354448675 5 56 -0.08210469450109592 6 1 0.16376336973135608 6 2 -0.14357390968401518 6 3 0.2811977274375863 6 4 0.37263590423386717 6 5 0.3541617886456123 6 6 0.3696840758796398 6 7 0.12643948715474096 6 8 0.1579302068961539 6 9 0.1872928251980781 6 10 0.3258274481870913 6 11 -0.23343794201263535 6 12 0.10895232226447149 6 13 0.14937374875341902 6 14 -0.17367866226766182 6 15 -0.24344411468049998 6 16 0.018421874616791456 6 17 0.2337525463973833 6 18 -0.006998844845291785 6 19 0.35777070902530284 6 20 0.19673715146386428 6 21 0.15958850303626093 6 22 0.17072176678453843 6 23 0.304850470219435 6 24 0.6402301095795551 6 25 -0.0020415991028173153 6 26 0.0424949480226573 6 27 0.5138628640688978 6 28 -0.3909042765923516 6 29 0.03271815442055015 6 30 0.06650331815884414 6 31 0.4181994817599346 6 32 0.5296574783747303 6 33 0.2760024577569382 6 34 0.5801304095025515 6 35 0.5290714482227372 6 36 0.8507040684176275 6 37 0.6726316871287779 6 38 1.3743197826160767 6 39 1.012027552798834 6 40 0.7091540066146251 6 41 1.4331750956734126 6 42 1.0442084981433268 6 43 1.3712496428943979 6 44 1.1675439659573144 6 45 1.1863586956096484 6 46 0.89792950047784 6 47 0.6471374165999393 6 48 0.6677253532698941 6 49 0.5137089118337137 6 50 0.5643934266999651 6 51 0.9087296488864958 6 52 1.3833232865939173 6 53 0.3012938724886496 6 54 1.0375482341293534 6 55 0.5343254695708062 6 56 1.1585994289616472 7 1 -0.6222784030653798 7 2 -1.0820724971892233 7 3 -1.1397720312805149 7 4 -0.6790475116879549 7 5 -0.9687206269520345 7 6 -0.7835137197022632 7 7 -0.377473818083061 7 8 -0.40081568297556913 7 9 -0.5377569594543559 7 10 -0.6123006488814013 7 11 -0.5676203159301068 7 12 -0.674588640084437 7 13 -0.15665135197366453 7 14 -0.8769158224584643 7 15 -0.7332787833231151 7 16 -0.2852876608808188 7 17 -0.44924657368783016 7 18 -0.21711323376762692 7 19 -0.16559334414007765 7 20 -0.3677050288723578 7 21 -0.7399159660046308 7 22 -0.3737602412736294 7 23 0.1563301164010653 7 24 -0.5499704183693197 7 25 -0.3814701176041808 7 26 -0.36768427672610215 7 27 -0.10615288177466198 7 28 -0.7606537324345268 7 29 -1.0395274807389967 7 30 -1.0236184805722317 7 31 -0.34964194833315076 7 32 -0.029181034106166237 7 33 -0.2823266303734755 7 34 -0.49748733698936576 7 35 -0.3890964765677012 7 36 -0.04625327887085007 7 37 -0.3562665669350409 7 38 -0.24342670930259658 7 39 -0.09999949300303967 7 40 -0.2727845845954846 7 41 0.11487749923011847 7 42 -0.2429679016158559 7 43 0.28858396420222476 7 44 0.1199359448393551 7 45 -0.1370331443384849 7 46 -0.6524340432631845 7 47 -0.4353893281784593 7 48 -0.325698441478405 7 49 -0.8721568780055868 7 50 -0.5085931893330091 7 51 -0.18986711858444494 7 52 -0.06084843572349996 7 53 -0.18321499612618197 7 54 -0.4703015319985582 7 55 -0.6107678477079403 7 56 0.015497497780247116 8 1 -0.42022407139894113 8 2 -0.6085850889809095 8 3 -0.4452187132645662 8 4 -0.4294191835355008 8 5 -0.34228462208650967 8 6 -0.23961139055868586 8 7 -0.006522788664675261 8 8 -0.11884064130326522 8 9 -0.11026675807579522 8 10 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120 5 -0.21955415058756955 120 6 -0.20123466055633799 120 7 -0.9444069078295811 120 8 -0.9478812230532085 120 9 -0.8842287566683353 120 10 -0.037374866739362744 120 11 -0.5342336979808024 120 12 -0.5209005834505049 120 13 -0.5096086481600111 120 14 -0.3240041988000968 120 15 -0.6899355542753938 120 16 -0.877888711348966 120 17 -0.8544075693489432 120 18 -0.5806665564440436 120 19 -0.22158786343672432 120 20 -0.8536607265202178 120 21 0.2236830291997549 120 22 0.11000317503146562 120 23 0.43342534362189156 120 24 -0.06273588993816759 120 25 0.18710718314941488 120 26 -0.2936034363515887 120 27 0.5248587647199727 120 28 0.3128333650763224 120 29 0.13115075630142314 120 30 -0.20868356392459475 120 31 0.1976062818563875 120 32 0.21347710702195144 120 33 0.1099547123988458 120 34 0.16681958239861672 120 35 0.5555484195272982 120 36 0.2516686211063117 120 37 0.29959093477767595 120 38 0.37383750627173384 120 39 0.14320671846076038 120 40 0.8828217182133713 120 41 0.4106527903924828 120 42 0.09185740096464201 120 43 0.8514521989590236 120 44 0.6448046363214317 120 45 0.31078599245073946 120 46 0.6649900261590567 120 47 0.3049623502869423 120 48 0.9487477791199055 120 49 0.6408369562031382 120 50 1.200564152525864 120 51 0.9916811801138898 120 52 1.6487199588871384 120 53 1.4544648394527186 120 54 1.336366657085705 120 55 2.2948270369689254 120 56 0.31099875377476494 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 95.0 3 9.0 4 24.0 5 31.0 6 70.0 7 158.0 8 120.0 9 192.0 10 235.0 11 229.0 12 299.0 13 595.0 14 661.0 15 628.0 16 712.0 17 751.0 18 916.0 19 900.0 20 1107.0 21 1195.0 22 1201.0 23 1544.0 24 1764.0 25 1948.0 26 2074.0 27 2409.0 28 2716.0 29 3164.0 30 3586.0 31 4331.0 32 5356.0 33 6540.0 34 8541.0 35 11942.0 36 16767.0 37 23567.0 38 28571.0 39 21250.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 50.78682185431311 39.78155930293602 2.770842136262948 6.66077670648792 11 32.047145289952496 22.85048464128862 24.920933686730944 20.18143638202794 12 30.097696513399658 17.137223267903558 27.467701250976322 25.29737896772046 13 23.566883058681928 20.00089629828807 29.420991305906607 27.011229337123392 14 20.689765553976365 23.798640187454385 33.17199964148069 22.33959461708857 15 23.30759676820446 27.956183817974622 29.44403897617127 19.29218043764965 16 28.940191295663197 28.1463270976581 24.30184765489955 18.61163395177915 17 28.022765976516983 27.13671109745323 23.838973610417547 21.001549315612237 18 26.321719868372195 27.72186583695054 23.986222614886234 21.970191679791036 19 24.356265765246672 31.249439813569957 24.23846656167172 20.155827859511646 20 25.913904147300222 30.517036069603964 23.522708357341322 20.04635142575449 21 24.45549878999731 29.721251232410147 25.46767564245381 20.355574335138733 22 23.856077339223404 28.5700566599443 27.021991741092865 20.551874259739428 23 24.080286830142775 30.226006786606057 25.42864459952622 20.26506178372495 24 24.982394140770047 28.75773057273461 24.916452195290592 21.343423091204752 25 22.5999807883193 33.235567224872725 21.66821427427876 22.49623771252922 26 23.60239689813183 30.871278879738522 24.373377767808506 21.152946454321146 27 19.7224536888661 27.86423947182873 24.56445099673098 27.848855842574196 28 22.02612954510488 31.422124211498026 24.353813016052108 22.197933227344993 29 26.984992303745507 28.830810672139556 22.37429451000513 21.8099025141098 30 23.42660933187857 29.865862268147104 26.00282395224954 20.704704447724794 31 26.79145723804572 29.20702781468857 23.593377883053773 20.40813706421194 32 27.650831765680277 28.358362278736205 24.825846051119402 19.16495990446412 33 29.575393231725723 27.407721407605802 22.965760419275902 20.051124941392576 34 24.343296810574046 30.33551271017713 26.686876212251608 18.63431426699721 35 21.423477026080587 33.420932578566244 27.579626428842214 17.575963966510958 36 22.39719641192168 34.89438317847153 23.962318747387712 18.74610166221908 37 26.14290029628963 33.225572173197676 22.808517202151794 17.823010328360894 38 22.336148616051617 35.53045458346467 22.907352969555063 19.226043830928656 39 25.522988320839097 31.55754319359094 23.120877706637497 19.798590778932468 40 24.167310664605875 30.964064914992274 23.903915507470376 20.964708912931478 41 22.776428760055907 32.56600346522218 23.767044320062862 20.89052345465905 42 20.61111003717951 31.621915936930144 23.443067664134336 24.323906361756006 43 20.612590783427954 30.95239629584281 24.4784987465926 23.956514174136633 44 22.00255184235285 28.910569524816022 23.45503988864688 25.63183874418425 45 23.971326719397652 29.138705705647634 24.217576566297293 22.67239100865742 46 22.215775766483585 32.71834145586757 23.200149557773138 21.865733219875715 47 20.352572372155425 31.53284483203468 24.97484390319418 23.139738892615718 48 21.831240401120247 30.043364350889874 26.664558677387294 21.460836570602584 49 24.620454809292248 29.07954648058096 24.90293704128884 21.397061668837953 50 21.36131601195201 29.912295986524946 26.346053319393626 22.38033468212942 51 20.511811531564074 30.503554425937633 26.880104245958535 22.104529796539758 52 19.54352400500884 31.691259052709036 26.287388817887248 22.477828124394872 53 19.746941673929182 29.488396113747132 28.39030373454698 22.374358477776703 54 20.051518438177872 30.01110422477017 27.952303481045348 21.98507385600661 55 20.603073504428092 30.054842481283146 26.77785888235028 22.56422513193848 56 19.739731335572202 31.81542237148024 23.80973908550762 24.635107207439937 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 346.0 7 692.0 8 2103.0 9 3514.0 10 4216.0 11 4918.0 12 3615.5 13 1818.0 14 1323.0 15 1170.5 16 1018.0 17 916.0 18 814.0 19 855.0 20 896.0 21 979.5 22 1336.0 23 1609.0 24 1876.5 25 2144.0 26 2516.0 27 2888.0 28 2949.0 29 2980.5 30 2951.0 31 3430.0 32 3909.0 33 3711.5 34 3514.0 35 3805.0 36 4096.0 37 4198.0 38 4633.5 39 4967.0 40 4857.5 41 4748.0 42 5025.5 43 5303.0 44 5230.5 45 5158.0 46 5494.0 47 6516.0 48 7202.0 49 7586.5 50 7971.0 51 7661.5 52 7352.0 53 7680.0 54 9169.5 55 10331.0 56 10275.5 57 10220.0 58 11150.0 59 12080.0 60 9687.0 61 7294.0 62 6110.5 63 4292.5 64 3658.0 65 2762.5 66 1867.0 67 1676.5 68 1486.0 69 1346.0 70 1206.0 71 954.0 72 557.5 73 413.0 74 323.5 75 234.0 76 185.5 77 137.0 78 97.5 79 48.0 80 38.0 81 29.5 82 21.0 83 13.5 84 6.0 85 7.0 86 8.0 87 4.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0019206391887220068 23 0.005121704503258684 24 0.0 25 0.027529161705015428 26 0.10435472925389569 27 0.12036005582657908 28 0.13188389095891112 29 0.17797923148823927 30 0.24840266840804617 31 0.2669688472323589 32 0.2848948129937643 33 0.32074674451657514 34 0.3175456792020384 35 0.3617203805426446 36 0.4372655219657102 37 0.38860932918475266 38 0.47183702736270633 39 0.507688958885517 40 0.5877155917489341 41 0.6024404921958028 42 0.6434141282218723 43 0.6536575372283896 44 0.6504564719138529 45 0.6856681903737564 46 0.6875888295624785 47 0.7464884313499532 48 0.7887424935018374 49 0.732403743965992 50 0.7970652633196327 51 0.755451414230656 52 0.8143510160181309 53 0.8277954903391848 54 0.8335574079053508 55 0.8905363705041037 56 0.8694093394281618 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 156198.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.234663696078055 #Duplication Level Percentage of deduplicated Percentage of total 1 62.75505406676069 17.09112792737423 2 10.580629995298542 5.763197992291834 3 6.036671368124118 4.932201436638113 4 4.2054536906441 4.581364678164893 5 2.983074753173484 4.0621518841470445 6 2.3648330982604606 3.864326047708677 7 1.7489421720733427 3.3342296316214033 8 1.4339445228020686 3.1242397469877976 9 1.1753643629525155 2.88095878308301 >10 6.133051245886225 28.138004327840306 >50 0.38316878232251994 7.096121589264907 >100 0.17865538316878232 8.745310439314204 >500 0.014104372355430182 2.8899217659637126 >1k 0.007052186177715091 3.496843749599867 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2248 1.4391989654156903 No Hit GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 1658 1.0614732583003623 No Hit GAATTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1556 0.9961715258838142 No Hit GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 890 0.5697896259875286 No Hit GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 888 0.568509199861714 No Hit GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC 861 0.5512234471632159 No Hit GAATTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 648 0.4148580647639535 No Hit GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 617 0.39501145981382607 No Hit GAATTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 610 0.3905299683734747 No Hit GAATTAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 455 0.2912969436228377 No Hit GAATTAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 449 0.2874556652453937 No Hit GAATTAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 437 0.27977310849050563 No Hit GAATTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 366 0.23431798102408483 No Hit GAATTAGGGGGTGCTCCACTCTTCTGGTTCTTCAACATCTATGACCTTTTTATGGC 365 0.23367776796117748 No Hit GAATTAGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCT 347 0.22215393282884544 No Hit GAATTAGGGATCTTGGTTTTGTCCACTTCATCTTTATATGACATGTGACCCATTGT 317 0.20294754094162537 No Hit GAATTAGGGAGGTTACGGCCATTTGTTTAGGGGCATGAAGGAACGTTTTTAATATA 316 0.20230732787871802 No Hit GAATTAGGGAGCCCAAGGCCAAGGACCCTTTCGCTCACCTGCCCAAGAGTACCTTT 313 0.200386688689996 No Hit GAATTAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTC 295 0.188862853557664 No Hit GAATTAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT 289 0.18502157518021997 No Hit GAATTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 277 0.17733901842533195 No Hit GAATTAGGGAACATAGACTCATTTATTTAAATGTTTGGCCCATAGGTAATCACATT 268 0.17157710085916594 No Hit GAATTAGGGACCTGACACAGTTCCCTCACCAAAAGCACATCCAGCCAGTGGCCTGA 264 0.1690162486075366 No Hit GAATTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 260 0.16645539635590725 No Hit GAATTAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 249 0.15941305266392655 No Hit GAATTAGGGGGCTCGCACTCCGGCTCTGCTACCCCACAATCGGGTGCGGATTGGTG 244 0.15621198734938987 No Hit GAATTAGGGGACAAAAATTATGGCAATGCTCTGGAAATATACTTAACGTTAAGACA 239 0.1530109220348532 No Hit GAATTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC 238 0.15237070897194588 No Hit GAATTAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 228 0.14596857834287252 No Hit GAATTAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC 215 0.13764580852507713 No Hit GAATTAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 206 0.13188389095891112 No Hit GAATTAGGGAGATCACTGTATAAGTTGTGAGGGTCAGCTGGACGGATGAGGAACTC 200 0.1280426125814671 No Hit GAATTAGGGGTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT 192 0.12292090807820844 No Hit GAATTAGGGGGGGGCTGGAGAAATGGCTCAGCGGTTAAGAGCACTGACTGTTCTTC 185 0.11843941663785706 No Hit GAATTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA 175 0.11203728600878372 No Hit GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCT 169 0.10819600763133971 No Hit GAATTAGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC 165 0.10563515537971037 No Hit GAATTAGGGATTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCCT 163 0.10435472925389569 No Hit GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTC 158 0.10115366393935901 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 6.402130629073355E-4 0.0 0.0 0.0 0.0 11 0.001280426125814671 0.0 0.0 0.0 0.0 12 0.0019206391887220066 0.0 0.0 0.0 0.0 13 0.002560852251629342 0.0 0.0 0.0 0.0 14 0.004481491440351349 0.0 0.0 0.0 0.0 15 0.00576191756616602 0.0 0.0 0.0 0.0 16 0.00576191756616602 0.0 0.0 0.0 0.0 17 0.006402130629073356 0.0 0.0 0.0 0.0 18 0.006402130629073356 0.0 0.0 0.0 0.0 19 0.007682556754888026 0.0 0.0 0.0 0.001280426125814671 20 0.009603195943610034 0.0 0.0 0.0 0.001280426125814671 21 0.010883622069424705 0.0 0.0 0.0 0.001280426125814671 22 0.010883622069424705 0.0 0.0 0.0 0.001280426125814671 23 0.01152383513233204 0.0 0.0 0.0 0.001280426125814671 24 0.014084687383961382 0.0 0.0 0.0 0.001280426125814671 25 0.015365113509776053 0.0 0.0 0.0 0.001280426125814671 26 0.017925965761405396 0.0 0.0 0.0 0.001280426125814671 27 0.02432809639047875 0.0 0.0 0.0 0.001280426125814671 28 0.03201065314536678 0.0 0.0 0.0 0.001280426125814671 29 0.03585193152281079 0.0 0.0 0.0 0.001280426125814671 30 0.04161384908897681 0.0 0.0 0.0 0.001280426125814671 31 0.04353448827769882 0.0 0.0 0.0 0.001280426125814671 32 0.04481491440351349 0.0 0.0 0.0 0.001280426125814671 33 0.04737576665514283 0.0 0.0 0.0 0.001280426125814671 34 0.04801597971805017 0.0 0.0 0.0 0.001280426125814671 35 0.05633874953584553 0.0 0.0 0.0 0.001280426125814671 36 0.05825938872456753 0.0 0.0 0.0 0.001280426125814671 37 0.059539814850382206 0.0 0.0 0.0 0.001280426125814671 38 0.06082024097619688 0.0 0.0 0.0 0.001280426125814671 39 0.06274088016491888 0.0 0.0 0.0 0.001280426125814671 40 0.06338109322782622 0.0 0.0 0.0 0.001280426125814671 41 0.06594194547945556 0.0 0.0 0.0 0.001280426125814671 42 0.07042343691980692 0.0 0.0 0.0 0.001280426125814671 43 0.07170386304562158 0.0 0.0 0.0 0.001280426125814671 44 0.07298428917143625 0.0 0.0 0.0 0.001280426125814671 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTACAC 30 1.0152125E-6 50.188972 41 GTACACC 30 1.0152125E-6 50.188972 42 ACGTTCA 15 0.008292768 50.188972 49 CCAATAA 15 0.008292768 50.188972 47 CGGATGA 30 1.0152125E-6 50.188972 43 TATTCGT 15 0.008292768 50.188972 50 ACGGATG 30 1.0152125E-6 50.188972 42 ACGTGAA 30 1.0152125E-6 50.188972 46 GACGGAT 30 1.0152125E-6 50.188972 41 CTCGATC 30 1.0152125E-6 50.188972 48 GTATATG 15 0.008292768 50.188972 44 ACCAATA 15 0.008292768 50.188972 46 GCATATA 15 0.008292768 50.188972 48 GGTAATC 40 2.570232E-9 50.18897 45 CGTGAAA 25 2.0331763E-5 50.18897 47 ACTCCGT 20 4.095676E-4 50.18897 49 AAGTCAC 20 4.095676E-4 50.18897 41 GATAAGT 15 0.008303348 50.172794 38 TAAGTCA 15 0.008303348 50.172794 40 GGACGGA 30 1.0174754E-6 50.172794 40 >>END_MODULE