FastQCFastQC Report
Tue 31 May 2016
SRR212836_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212836_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307966
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27690.8991252281095966No Hit
ACGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21700.704623237630128No Hit
ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT21430.6958560360559283No Hit
ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14480.4701817733126384No Hit
ACGTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC10490.340622016716131No Hit
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC9390.3049037880805024No Hit
ACGTTGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG9210.2990589870310359No Hit
ACGTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT8100.2630160472259925No Hit
ACGTTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT7180.23314261963983035No Hit
ACGTTGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA6790.22047888403265292No Hit
ACGTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6630.21528350532201607No Hit
ACGTTGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA6390.20749043725606073No Hit
ACGTTGGGGATCTATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGACT5640.18313709954995033No Hit
ACGTTGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA5410.17566874265340982No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA5320.17274634212867654No Hit
ACGTTGGGGATCGGTGTACTCCGAAAAGGGAGAGTCATCTGGCAAGAATGTCACTTTG4600.14936713793081052No Hit
ACGTTGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4480.1454706038978329No Hit
ACGTTGGGGGCTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4470.14514589272841807No Hit
ACGTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA4400.14287291454251444No Hit
ACGTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG4320.14027522518719598No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA3910.12696206724118897No Hit
ACGTTGGGGGTGCAGTGCTACGTTGGGGGTGCAGTGCTACGTTGGGGTCATGGCTTGT3830.12436437788587051No Hit
ACGTTGGGGAGTATAAGAGATATGCAGTTAGTTTTGGAGGTAGTTTGTAGCCTTACAT3730.12111726619172246No Hit
ACGTTGGGGGTTTCTTCCAGGTAGTACTTATTATTTATCTTAAAATCACCTGAAAATA3600.11689602098933No Hit
ACGTTGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3570.11592188748108558No Hit
ACGTTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3510.11397362046459675No Hit
ACGTTGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG3500.11364890929518193No Hit
ACGTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3500.11364890929518193No Hit
ACGTTGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG3410.11072650877044868No Hit
ACGTTGGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA3360.10910295292337466No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3330.10812881941513024No Hit
ACGTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGC3320.10780410824571544No Hit
ACGTTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAAG3230.1048817077209822No Hit
ACGTTGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3190.10358286304332297No Hit
ACGTTGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA3130.10163459602683413No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCT150.007178661452.0666952
ACGTATC150.007178661452.0666951
GTTCGCG150.007192525552.04129448
AGGCGGT150.00719715152.03283742
AATGCGA150.00719715152.03283741
TACGCCA150.00720177952.0243838
TATCGGT150.00720177952.0243837
TCGCCTA150.007211041652.00747733
TAAAGCG150.007211041652.00747732
TAGCGAA150.007211041652.00747732
CGCCTAG150.007211041652.00747734
AGGGCGA203.4412538E-452.00747335
CATAGCG150.00722031351.9905830
CGTGCGG150.00722495251.9821427
CGCGATC150.00722495251.9821425
GCGTGCG150.00722495251.9821426
CGATATT150.00722495251.9821424
TCTATAC150.00722495251.9821426
CAACGCG459.276846E-1151.98213622
TAAGGCG459.276846E-1151.98213624
Produced by FastQC (version 0.11.5)