FastQCFastQC Report
Tue 31 May 2016
SRR212835_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212835_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307788
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27660.8986705134703108No Hit
ACGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20670.671566142929549No Hit
ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT20610.6696167491910016No Hit
ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14450.4694789920334776No Hit
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC9780.3177511793832118No Hit
ACGTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC9490.30832910964689986No Hit
ACGTTGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG8620.2800629004379638No Hit
ACGTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT7630.24789790375193316No Hit
ACGTTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6960.22612967367148817No Hit
ACGTTGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA6580.21378351332735518No Hit
ACGTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6110.19851326237540126No Hit
ACGTTGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA5750.18681689994411738No Hit
ACGTTGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA5380.17479563855640895No Hit
ACGTTGGGGATCTATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGACT5090.16537356882009693No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA4640.1507531157809921No Hit
ACGTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA4620.15010331786814302No Hit
ACGTTGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4520.1468543283038975No Hit
ACGTTGGGGATCGGTGTACTCCGAAAAGGGAGAGTCATCTGGCAAGAATGTCACTTTG4390.1426306418703783No Hit
ACGTTGGGGGCTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4110.13353347109049085No Hit
ACGTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG4060.1319089763083681No Hit
ACGTTGGGGGTTTCTTCCAGGTAGTACTTATTATTTATCTTAAAATCACCTGAAAATA4000.1299595825698208No Hit
ACGTTGGGGAGTATAAGAGATATGCAGTTAGTTTTGGAGGTAGTTTGTAGCCTTACAT3660.11891301805138602No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3570.11598892744356505No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA3490.11338973579216864No Hit
ACGTTGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3440.11176524101004588No Hit
ACGTTGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG3410.11079054414077222No Hit
ACGTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3380.10981584727149855No Hit
ACGTTGGGGGCCCTAAAGAATAAGGCTCTATTTTTTAATTTTTACGTGCCCCCCTTTA3360.10916604935864946No Hit
ACGTTGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3260.10591705979440394No Hit
ACGTTGGGGGTGCAGTGCTACGTTGGGGGTGCAGTGCTACGTTGGGGTCATGGCTTGT3210.10429256501228118No Hit
ACGTTGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG3180.10331786814300752No Hit
ACGTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGC3170.10299296918658297No Hit
ACGTTGGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA3140.10201827231730931No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCC150.00713326952.1502527
AACGACG251.6290682E-552.12473725
GACGACA150.007156308752.10774221
CACAAAT150.007156308752.10774221
GTAGTAC251.6322158E-552.10774221
TAGTACT251.6322158E-552.10774222
TGCGCAC150.00718865752.0483652
TATCTAC251.644857E-552.03988350
CGGTTAG353.857531E-852.02294549
ACGGATA150.007202553652.02294549
GCGTAAG251.6496184E-552.01448444
AACGTTC150.0072071952.0144846
TCCGACG203.4389584E-452.0144844
AAACGCT150.0072071952.0144845
AACCGCT150.00721182952.0060238
ACACGAT150.00721182952.0060241
TCGTGTA150.00721182952.0060241
ACAATCG150.00721182952.0060241
CATACGT150.00721182952.0060237
CTACCGC150.00721182952.0060238
Produced by FastQC (version 0.11.5)