FastQCFastQC Report
Tue 31 May 2016
SRR212834_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212834_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306126
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24750.8084906215087905No Hit
ACGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18280.5971397398456845No Hit
ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT18140.5925664595624024No Hit
ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13120.42858169511900324No Hit
ACGTTGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG9050.2956299040264466No Hit
ACGTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC8770.2864833434598825No Hit
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC8480.27701012001594116No Hit
ACGTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT7810.2551237072316628No Hit
ACGTTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6730.21984411647491556No Hit
ACGTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6030.19697771505850534No Hit
ACGTTGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA5810.18979113175620496No Hit
ACGTTGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA5770.1884844802466958No Hit
ACGTTGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA5240.17117134774569948No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA4820.15745150689585333No Hit
ACGTTGGGGGCTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4490.14667163194240282No Hit
ACGTTGGGGATCTATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGACT4260.13915838576272516No Hit
ACGTTGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3910.12772518505452002No Hit
ACGTTGGGGATCGGTGTACTCCGAAAAGGGAGAGTCATCTGGCAAGAATGTCACTTTG3790.12380523052599257No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3700.12086526462959696No Hit
ACGTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3670.1198852759974651No Hit
ACGTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA3640.11890528736533323No Hit
ACGTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG3630.11857862448795595No Hit
ACGTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGC3250.10616543514761895No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA3190.10420545788335522No Hit
ACGTTGGGGGTGCAGTGCTACGTTGGGGGTGCAGTGCTACGTTGGGGTCATGGCTTGT3170.10355213212860065No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTTCG150.00701006152.3803989
GTACGTA150.007171510752.0797139
CAACGGC150.0072041352.01998524
ACACGAT203.4510487E-451.97741341
TAACACG203.4510487E-451.97741340
AGCCGGA251.6565697E-551.9774144
TACGTAG150.007227496751.9774140
ATAGATC150.007236859351.960430
GCGTTAT203.4790317E-451.89246732
TAGTACT505.456968E-1251.89246722
GTTAGAC150.007274399551.89246431
TCTAATC150.007274399551.89246432
CGCATCG203.4818397E-451.88398720
GTAGTAC505.456968E-1251.88398721
CAGTATA150.00727910251.88398419
ACCGCAT150.007283807351.87550718
ATTCGAC251.6775486E-551.86703512
TTGGGGC34500.049.994094
ACGTTGG317600.049.9889831
CGTTGGG318150.049.902562
Produced by FastQC (version 0.11.5)