FastQCFastQC Report
Tue 31 May 2016
SRR212833_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212833_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288098
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26830.9312803282216469No Hit
ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT20620.7157286756589771No Hit
ACGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20470.710522114002874No Hit
ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14460.5019125436483419No Hit
ACGTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA8340.2894848280793341No Hit
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG8050.27941880887753473No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC6510.22596477587487593No Hit
ACGTTGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA6010.20860957035453215No Hit
ACGTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6000.20826246624412528No Hit
ACGTTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC5670.19680803060069837No Hit
ACGTTGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC5660.1964609264902915No Hit
ACGTTGGGGAGTATAAGAGATATGCAGTTAGTTTTGGAGGTAGTTTGTAGCCTTAC5440.18882463606134023No Hit
ACGTTGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC5180.1797999291907615No Hit
ACGTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5120.1777173045283202No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC4690.16279182778082457No Hit
ACGTTGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC4420.15342001679983894No Hit
ACGTTGGGGATCGGTGTACTCCGAAAAGGGAGAGTCATCTGGCAAGAATGTCACTT4380.15203160035821145No Hit
ACGTTGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC4220.14647793459170141No Hit
ACGTTGGGGATCTATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGA3900.13537060305868143No Hit
ACGTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA3770.13085824962339204No Hit
ACGTTGGGGGTGAGAGGGACAAAGAAAGAAAGGAAGGAAGGGGGGGAGATTATTTT3530.12252775097362702No Hit
ACGTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3420.11870960575915139No Hit
ACGTTGGGGGTTTCTTCCAGGTAGTACTTATTATTTATCTTAAAATCACCTGAAAA3400.11801539753833765No Hit
ACGTTGGGGACTGCTCACAACAAAAACAGACAAGGCCCACAGGTGCATCGGTTAGA3310.11489146054467576No Hit
ACGTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAG3110.10794937833653827No Hit
ACGTTGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3100.10760227422613139No Hit
ACGTTGGGGGCTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC3090.10725517011572451No Hit
ACGTTGGGGGTGCAGTGCTACGTTGGGGGTGCAGTGCTACGTTGGGGTCATGGCTT2930.1017015043492145No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACATC150.00823888950.2827150
CTTCGAA150.00825030550.265148
CCGGCGT150.00825030550.265149
CCTAATA150.00825030550.265149
ACCTAAT150.00825030550.265148
TGATTCG150.00825030550.265148
TGTAGCG204.0991866E-450.1947942
GTAGTCT204.0991866E-450.1947943
CTCAAAT204.1133564E-450.1597139
AAAGGAT150.00831905250.15970239
TTAAGCG301.0248332E-650.1421838
ACCCGTT204.1240093E-450.13342736
CTACCCG204.1311234E-450.1159234
ACTGACG204.148951E-450.07221232
TGCGATC252.0658099E-550.07221232
CTACGAC150.00837666650.07221232
GTGCGAT301.034754E-650.07221231
CGGGCAC204.152524E-450.0634830
TCGGGCA150.00839400850.04602429
CTCACGT150.00839979550.037327
Produced by FastQC (version 0.11.5)