SRR212832_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212832_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269149
Sequences flagged as poor quality	0
Sequence length	56
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2482	0.9221657892096943	No Hit
ACGTTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	1844	0.685122367164656	No Hit
ACGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1763	0.6550275126416966	No Hit
ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1220	0.45328052491370957	No Hit
ACGTTGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	1099	0.40832401383620226	No Hit
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	762	0.2831145573641366	No Hit
ACGTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA	749	0.27828451898390855	No Hit
ACGTTGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA	713	0.2649090280848155	No Hit
ACGTTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	624	0.2318418422509465	No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	611	0.22701180387071843	No Hit
ACGTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	564	0.20954935741912475	No Hit
ACGTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	551	0.20471931903889667	No Hit
ACGTTGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	516	0.1917153695536673	No Hit
ACGTTGGGGAGTATAAGAGATATGCAGTTAGTTTTGGAGGTAGTTTGTAGCCTTAC	512	0.19022920389821252	No Hit
ACGTTGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC	490	0.1820552927932112	No Hit
ACGTTGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC	464	0.1723952160327551	No Hit
ACGTTGGGGATCGGTGTACTCCGAAAAGGGAGAGTCATCTGGCAAGAATGTCACTT	416	0.15456122816729767	No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	386	0.14341498575138678	No Hit
ACGTTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT	375	0.13932803019888612	No Hit
ACGTTGGGGATCTATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGA	371	0.13784186454343134	No Hit
ACGTTGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	355	0.1318972019216122	No Hit
ACGTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAG	337	0.12520945647206566	No Hit
ACGTTGGGGGCTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC	336	0.12483791505820196	No Hit
ACGTTGGGGGTGCAGTGCTACGTTGGGGGTGCAGTGCTACGTTGGGGTCATGGCTT	334	0.12409483223047457	No Hit
ACGTTGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	334	0.12409483223047457	No Hit
ACGTTGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	321	0.11926479385024652	No Hit
ACGTTGGGGACTGCTCACAACAAAAACAGACAAGGCCCACAGGTGCATCGGTTAGA	314	0.11666400395320065	No Hit
ACGTTGGGGGTGAGAGGGACAAAGAAAGAAAGGAAGGAAGGGGGGGAGATTATTTT	305	0.11332013122842739	No Hit
ACGTTGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA	289	0.10737546860660824	No Hit
ACGTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	282	0.10477467870956238	No Hit
ACGTTGGGGGTTTCTTCCAGGTAGTACTTATTATTTATCTTAAAATCACCTGAAAA	281	0.10440313729569867	No Hit
ACGTTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC	270	0.100316181743198	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACGC	15	0.008233891	50.289516	50
GAAACCG	20	4.0644943E-4	50.280087	49
ACTAGTC	15	0.008252217	50.261246	48
GTGCATC	45	1.2914825E-10	50.223595	43
ACGATAT	25	2.0288744E-5	50.223595	43
ATACGAC	15	0.008276701	50.223595	43
CAATACT	20	4.08711E-4	50.22359	42
TTTACGT	30	1.0144831E-6	50.21419	41
TTAGCGT	15	0.00828283	50.21419	41
AACTCGG	15	0.008338146	50.12972	35
AAACTCG	15	0.008350476	50.11099	34
CCATCGA	20	4.1326415E-4	50.110985	34
TGCGATC	40	2.6357156E-9	50.073563	32
CGAGTGC	15	0.008393736	50.045532	31
GTATCAT	15	0.008393736	50.045532	30
CGATATA	15	0.008393736	50.045532	31
CTAGCAG	15	0.008393736	50.045532	31
CGTGCTA	25	2.0719672E-5	50.04553	31
ACCGACG	20	4.1593873E-4	50.04553	31
GTGCGAT	40	2.6484486E-9	50.04553	31

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content