FastQCFastQC Report
Tue 31 May 2016
SRR212831_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212831_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163792
Sequences flagged as poor quality0
Sequence length58
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17551.071480902608186No Hit
TATTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11430.6978362801602033No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7660.4676663084888151No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT7300.4456872130506984No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC4640.28328611898017No Hit
TATTGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4040.24665429324997556No Hit
TATTGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA3180.19414867637003028No Hit
TATTGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3130.1910960242258474No Hit
TATTGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2750.16789586793005765No Hit
TATTGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2700.16484321578587477No Hit
TATTGTGGGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGAGTTTGCGGA2540.1550747289244896No Hit
TATTGTGGGGACGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTCT2480.15141154635147017No Hit
TATTGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2460.15019048549379702No Hit
TATTGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC2320.141643059490085No Hit
TATTGTGGGATTGCCTACTGAGGACAACTGAGCAGTCTGTCGGGGGCTGACATTGCCC2190.13370616391520954No Hit
TATTGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC2090.1276008596268438No Hit
TATTGTGGGATAATTATTTTTTTCTGGTAAATTCATGTCAGTAATTTGTTGAAGAGTT2000.12210608576731465No Hit
TATTGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2000.12210608576731465No Hit
TATTGTGGGATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGCTT1930.11783237276545863No Hit
TATTGTGGGAGTCTTTATTTTTATACATTCCCTTATGTGGCTTAGCCCAGGCTGGCCG1860.11355865976360262No Hit
TATTGTGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1790.10928494676174662No Hit
TATTGTGGGACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCATCT1770.10806388590407347No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA1710.10440070333105402No Hit
TATTGTGGGGTAGGTGCTACCACGAACATCCATCCGTACTTGTCCACGATGATCAACT1660.10134805118687115No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGCG203.4086665E-452.0931149
TAACGCT150.007158290652.0931152
CATAACG150.007158290652.0931150
ATTGCCC401.8480932E-952.0931152
TATCATA150.007166967352.07715647
TAAAACG251.6344944E-552.07715647
GTAGCGC150.007166967352.07715643
ACATATT251.6374606E-552.0612238
CTAAGCA150.00717565252.06121437
TATCTAC150.00717565252.06121439
TGTAGCG150.00717565252.06121442
CCTTATG251.6404312E-552.04528831
TTCGTTG307.905055E-752.04528834
CGTTAAA251.6404312E-552.04528831
CTCGACA150.00720175352.01345430
CCGTTAA251.6463859E-552.01345430
GCCGTTA251.64937E-551.99755529
ACGATCT150.007210469351.9975525
TAACCCG150.007210469351.9975528
TCTCGAC150.007210469351.9975529
Produced by FastQC (version 0.11.5)