FastQCFastQC Report
Tue 31 May 2016
SRR212830_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212830_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173939
Sequences flagged as poor quality0
Sequence length58
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18891.0860129125727984No Hit
TATTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11590.6663255509115265No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8260.4748791242906996No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT7680.4415341010354205No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC4630.26618527184817664No Hit
TATTGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4200.24146396150374555No Hit
TATTGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3550.20409453889007065No Hit
TATTGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3500.201219967919788No Hit
TATTGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2780.1598261459477173No Hit
TATTGTGGGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGAGTTTGCGGA2680.15407700400715194No Hit
TATTGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2570.14775294787253002No Hit
TATTGTGGGATTGCCTACTGAGGACAACTGAGCAGTCTGTCGGGGGCTGACATTGCCC2450.1408539775438516No Hit
TATTGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2330.13395500721517314No Hit
TATTGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2270.1305055220508339No Hit
TATTGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC2170.12475638011026854No Hit
TATTGTGGGGACGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTCT2130.1224567233340424No Hit
TATTGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC2090.12015706655781623No Hit
TATTGTGGGATAATTATTTTTTTCTGGTAAATTCATGTCAGTAATTTGTTGAAGAGTT2060.11843232397564664No Hit
TATTGTGGGATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGCTT1990.11440792461725087No Hit
TATTGTGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1950.11210826784102473No Hit
TATTGTGGGACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCATCT1940.11153335364696819No Hit
TATTGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1800.10348455493017668No Hit
TATTGTGGGAGTCTTTATTTTTATACATTCCCTTATGTGGCTTAGCCCAGGCTGGCCG1760.10118489815395051No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC150.00719269352.03138749
TACCCGT150.00719269352.03138734
CGGCCAA150.00719269352.03138738
TCGGGTA150.00719269352.03138748
TTGTACG150.00719269352.03138737
CATTCCG150.00719269352.03138735
TGACCGC150.00719269352.03138729
GTTCGAG150.00719269352.03138752
TCCACTA150.00719269352.03138750
TGTAGCG150.00719269352.03138741
TATACAG150.00719269352.03138733
CGTTTCC150.00719269352.03138742
TACGTTT150.00719269352.03138740
CCCGAGC150.00719269352.03138739
TCGTTCG203.4292828E-452.03138447
CGTTAAA203.4292828E-452.03138431
ACTAGCT203.4292828E-452.03138451
ACAATGA401.869921E-952.03138444
CGGTGTG203.4292828E-452.03138433
TACTAGC203.4292828E-452.03138450
Produced by FastQC (version 0.11.5)