FastQCFastQC Report
Tue 31 May 2016
SRR212829_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212829_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181432
Sequences flagged as poor quality0
Sequence length58
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17000.9369901671149521No Hit
TATTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11420.6294369240266325No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7470.4117245028440407No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT6850.3775519202786719No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC4450.2452709555095022No Hit
TATTGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4400.24251510207681115No Hit
TATTGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3400.18739803342299044No Hit
TATTGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2960.1631465232153093No Hit
TATTGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2830.15598130429031262No Hit
TATTGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2640.14550906124608667No Hit
TATTGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2400.13228096476916973No Hit
TATTGTGGGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGAGTTTGCGGA2390.13172979408263152No Hit
TATTGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC2180.12015520966532915No Hit
TATTGTGGGATTGCCTACTGAGGACAACTGAGCAGTCTGTCGGGGGCTGACATTGCCC2170.11960403897879096No Hit
TATTGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2100.1157458441730235No Hit
TATTGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC2090.11519467348648531No Hit
TATTGTGGGGACGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTCT2050.11298999074033247No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATTA150.007145094752.11966742
GTAGAAT150.007145094752.11966742
TAACGCT150.007168611552.07639352
CTAACGC150.007168611552.07639352
CGCTAGG203.4150487E-452.0763952
TCGCAAA150.007184321552.04757750
CGGGATC150.007184321552.04757751
CATAACG150.007184321552.04757750
GCGCTAG203.4243954E-452.04757751
GTCAAAC150.007200056752.018825
ACCGTCG150.007200056752.018826
CGTCAAA150.007200056752.018824
TAGGTGT150.00722370851.97569346
TCGATCT150.007231604351.96133847
AGGGATA150.007231604351.96133849
GTTACCA150.00724741751.93265511
TCACCGC203.4667074E-451.91832435
ACAGCTA150.007255332551.91832416
TCGTAGA150.007255332551.91832430
CGACTGG203.4667074E-451.91832415
Produced by FastQC (version 0.11.5)