FastQCFastQC Report
Tue 31 May 2016
SRR212828_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212828_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397131
Sequences flagged as poor quality0
Sequence length56
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44731.1263285918248638No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT34860.8777959917508328No Hit
TATTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29000.7302376293968489No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17870.4499774633559203No Hit
TATTGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10370.26112290402914906No Hit
TATTGTGGGATAATTATTTTTTTCTGGTAAATTCATGTCAGTAATTTGTTGAAGAG9670.24349647849198375No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG8170.20572556662662952No Hit
TATTGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG5760.14504030156296033No Hit
TATTGTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC5760.14504030156296033No Hit
TATTGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC5560.14000417998091308No Hit
TATTGTGGGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGAGTTTGCG5170.130183742895921No Hit
TATTGTGGGATGTGTATGTGTGTGTATGTATGTATATGTGTATGTGTGTGTATTGT5040.1269102638675903No Hit
TATTGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA4840.12187414228554305No Hit
TATTGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4760.11985969365272417No Hit
TATTGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA4730.11910427541541707No Hit
TATTGTGGGATTGCCTACTGAGGACAACTGAGCAGTCTGTCGGGGGCTGACATTGC4720.11885246933631471No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4700.11834885717811No Hit
TATTGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4650.11708982678259819No Hit
TATTGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA4530.11406815383336985No Hit
TATTGTGGGAGTCTTTATTTTTATACATTCCCTTATGTGGCTTAGCCCAGGCTGGC4470.11255731735875568No Hit
TATTGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC4310.1085284200931179No Hit
TATTGTGGGGAAATCAATCAGTTCGTGGGACCGCTGGAAAAATTCTTCACTGAAGA4240.10676577753940136No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA4200.10575855322299192No Hit
TATTGTGGGGACGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCT4190.10550674714388955No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTC204.0881056E-450.2270644
AAACCGT150.008314786550.16975438
TACCCTA204.1112027E-450.1697538
GAGCGTA150.00831895450.16339536
GAGCAAT150.00831895450.16339536
GCTTGCG355.169386E-850.1316235
TGCGATC402.6429916E-950.0681932
ACGTCAA150.00839423950.049230
CCGTTAA451.3460522E-1050.04919430
ACCGCGA150.00841103750.0238928
TACGGGA150.00841524150.0175727
CGGTACT204.1759055E-450.01124626
ACGGTAC150.00843628149.9859825
CACGGTA150.00845314349.9607424
CTTTACG150.00845314349.9607424
CACGATC204.1967805E-449.96073524
ACGTTAT150.008482709549.91662623
GCACGGT150.008482709549.91662623
AACGCGA301.0582353E-649.91662623
GCTATAT150.008512353549.8725921
Produced by FastQC (version 0.11.5)