FastQCFastQC Report
Tue 31 May 2016
SRR212827_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212827_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356316
Sequences flagged as poor quality0
Sequence length56
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38961.093411466226608No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT30290.8500881240247421No Hit
TATTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25900.7268828792420212No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17380.4877692834450319No Hit
TATTGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9490.2663366225485243No Hit
TATTGTGGGATAATTATTTTTTTCTGGTAAATTCATGTCAGTAATTTGTTGAAGAG7930.222555259937808No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG7640.21441641688837998No Hit
TATTGTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC6860.1925257355830218No Hit
TATTGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG6060.17007375475701345No Hit
TATTGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC5330.1495863222532808No Hit
TATTGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA4750.13330863615442473No Hit
TATTGTGGGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGAGTTTGCG4710.13218603711312432No Hit
TATTGTGGGATTGCCTACTGAGGACAACTGAGCAGTCTGTCGGGGGCTGACATTGC4520.12685369166694732No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4370.12264394526207073No Hit
TATTGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA4360.12236329550174566No Hit
TATTGTGGGAGTCTTTATTTTTATACATTCCCTTATGTGGCTTAGCCCAGGCTGGC4320.12124069646044522No Hit
TATTGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC4120.11562770125394313No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA4030.11310185341101718No Hit
TATTGTGGGATGTGTATGTGTGTGTATGTATGTATATGTGTATGTGTGTGTATTGT4010.11254055389036699No Hit
TATTGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3980.11169860460939166No Hit
TATTGTGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT3930.11029535580776614No Hit
TATTGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG3880.10889210700614062No Hit
TATTGTGGGGAAATCAATCAGTTCGTGGGACCGCTGGAAAAATTCTTCACTGAAGA3860.1083308074854904No Hit
TATTGTGGGAGTTTCCTTGATGTGACTTACCTCTTTTTGTTTATTTCTTTTGAGAC3800.10664690892353977No Hit
TATTGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA3660.10271781227898832No Hit
TATTGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3640.1021565127583381No Hit
TATTGTGGGATCAAGAAGAACCGCCATCCTGACTTCCTACCCTATGACCATGCCCG3590.10075326395671258No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATAC204.0896333E-450.2218345
CGAGTTA252.044275E-550.1650940
ACATTCG150.00833108150.1438538
TGACGCT150.00833573150.1367737
CTTGCGA204.126863E-450.12969236
GTCGGTA150.00834038350.12969236
TAACCCG150.00841976150.00971228
CGCGATC252.0908292E-549.97453325
AACGCGA252.1047905E-549.9183523
ATCGAAC150.00849498249.89731621
CGAACGC252.1223506E-549.848315
TACGAAC252.1223506E-549.848313
ACGAACG252.1223506E-549.848314
GTGGGAT59150.047.446485
GGGATAG3050.047.3967487
TGGGATT20200.046.8870126
ATTGTGG380650.046.659532
TTGTGGG381150.046.598323
TATTGTG381450.046.5616721
TGTGGGA189950.046.5285764
Produced by FastQC (version 0.11.5)