FastQCFastQC Report
Tue 31 May 2016
SRR212826_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212826_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276779
Sequences flagged as poor quality0
Sequence length58
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT15870.5733816510645677No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA10760.3887578176089949No Hit
TGGATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC8920.3222787856015088No Hit
TGGATGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT8530.3081881212086177No Hit
TGGATGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG7710.2785615960748467No Hit
TGGATGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG7330.26483223076895285No Hit
TGGATGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC7060.2550771554200283No Hit
TGGATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC6940.25074156637606176No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA6750.2438768837231148No Hit
TGGATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT6520.23556700472217906No Hit
TGGATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGC6270.22653452754724887No Hit
TGGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6250.22581192937325445No Hit
TGGATGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA6160.2225602375902796No Hit
TGGATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC5820.21027606863237455No Hit
TGGATGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT5630.20341138597942762No Hit
TGGATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG5610.2026887878054332No Hit
TGGATGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA5450.1969080024134779No Hit
TGGATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5380.19437890880449746No Hit
TGGATGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG5200.18787552523854772No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA5160.1864303288905589No Hit
TGGATGGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGGGC4970.17956564623761195No Hit
TGGATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT4930.17812044988962314No Hit
TGGATGGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG4860.17559135628064268No Hit
TGGATGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC4770.1723396644976678No Hit
TGGATGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGGT4700.16981057088868737No Hit
TGGATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCG4520.16330718732273763No Hit
TGGATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAAG4390.15861029919177394No Hit
TGGATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4280.15463600923480467No Hit
TGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4170.1506617192778354No Hit
TGGATGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG4060.14668742932086612No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC3860.13946144758092197No Hit
TGGATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGC3810.13765495214593593No Hit
TGGATGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA3800.13729365305893873No Hit
TGGATGGGGAAACAGTAAGCTCCTGGGTAAAACCTTGCTCTCAGATCAATAGATTATC3430.12392558684004204No Hit
TGGATGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3150.11380921240412024No Hit
TGGATGGGGGCTCTTTCCGAGCTGCTAGCGCTCCTGCGAACATGGTGAACGTGCCTAA2890.10441543614219287No Hit
TGGATGGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACT2880.10405413705519566No Hit
TGGATGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAA2880.10405413705519566No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA2800.101163744359218No Hit
TGGATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCT2790.10080244527222079No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA2770.10007984709822638No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAC150.007173198352.0753152
GTAAGGG150.007173198352.0753152
ACGCGCC150.007173198352.0753152
TCCGATG203.4369435E-452.01880643
TACCCGT203.4400137E-452.00940335
CCTAGAC203.4400137E-452.00940342
ACGAATA203.4400137E-452.00940336
GTCGAGT150.007209216252.009436
CGAAATC307.963008E-752.009431
GTTACGA150.007209216252.009437
GTATATC150.007209216252.009442
TAGGATC307.963008E-752.009434
CGCTCAC150.00721437352.027
GTAAGCT401.891749E-951.9812115
CTTCGAT251.655445E-551.98120518
CGAGAAT150.007229858551.97181311
TGGATGG280300.051.313591
GATGGGG280400.051.2952963
GGATGGG281000.051.185772
ATGGGGG117900.051.0901874
Produced by FastQC (version 0.11.5)