FastQCFastQC Report
Tue 31 May 2016
SRR212825_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212825_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295425
Sequences flagged as poor quality0
Sequence length58
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT17130.5798425996445798No Hit
TGGATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC10630.3598205974443598No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA10440.3533891850723534No Hit
TGGATGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT8490.2873825844122874No Hit
TGGATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC8100.2741812642802742No Hit
TGGATGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG7730.2616569349242617No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA7670.2596259625962596No Hit
TGGATGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC7510.2542100363882542No Hit
TGGATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT7360.24913260556824915No Hit
TGGATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGC7190.2433781839722434No Hit
TGGATGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG7180.24303968858424302No Hit
TGGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6970.23593128543623593No Hit
TGGATGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA6960.2355927900482356No Hit
TGGATGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA5900.19971227892019972No Hit
TGGATGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT5870.1986967927561987No Hit
TGGATGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG5680.19226538038419225No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA5660.19158838960819158No Hit
TGGATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5640.1909113988321909No Hit
TGGATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG5490.18583396801218585No Hit
TGGATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC5360.18143352796818144No Hit
TGGATGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGGT4980.16857070322416856No Hit
TGGATGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC4960.1678937124481679No Hit
TGGATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT4880.1651857493441652No Hit
TGGATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAAG4870.16484725395616484No Hit
TGGATGGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG4790.16213929085216214No Hit
TGGATGGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGGGC4640.15706186003215705No Hit
TGGATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4630.15672336464415673No Hit
TGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4370.14792248455614793No Hit
TGGATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCG4280.14487602606414487No Hit
TGGATGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG4220.14284505373614284No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC4030.13641364136413642No Hit
TGGATGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA4000.1353981552001354No Hit
TGGATGGGGAAACAGTAAGCTCCTGGGTAAAACCTTGCTCTCAGATCAATAGATTATC3950.13370567826013371No Hit
TGGATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGC3490.11813489041211814No Hit
TGGATGGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACT3230.10933401032410933No Hit
TGGATGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3160.10696454260810696No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA3130.10594905644410593No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA3070.10391808411610393No Hit
TGGATGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA3040.1029025979521029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCAA550.052.05884652
TCTCACG401.8681021E-952.05884652
ACTAGGC150.0071826552.0588451
GCAAGCG459.094947E-1152.0500249
ATTAGCG251.6428014E-552.0500249
ACACGAC203.430027E-452.04119547
CGGGCAT150.007192289552.04119545
TGACTAA150.007192289552.04119546
ATACTCG203.432899E-452.0323744
ATAGGTC150.007201938452.0235639
TATACGA307.949893E-752.0235627
TTCGAAC307.949893E-752.0235627
ACTAAAC203.435773E-452.02355627
CATTCGA401.882654E-952.01474425
CGCTTGT203.438649E-452.01474433
CGAATAC150.007206766452.0147437
GTATACG307.9592064E-752.0147426
ACGAATA150.007206766452.0147436
CCATTCG307.9592064E-752.0147424
TATATTG203.4415268E-452.0059328
Produced by FastQC (version 0.11.5)