##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR212824_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	308232
Sequences flagged as poor quality	0
Sequence length	58
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.39165952918581	38.0	36.0	39.0	33.0	40.0
2	36.22259207350307	38.0	35.0	39.0	30.0	40.0
3	36.99443276493031	38.0	36.0	39.0	33.0	40.0
4	34.110024267434916	36.0	32.0	39.0	25.0	40.0
5	35.8474331023385	38.0	35.0	39.0	28.0	40.0
6	36.88350009084066	38.0	36.0	39.0	33.0	40.0
7	37.54449246022477	38.0	38.0	39.0	34.0	40.0
8	37.49548067689273	38.0	38.0	39.0	34.0	40.0
9	37.44033066002232	38.0	37.0	39.0	34.0	40.0
10	35.139103013314646	38.0	34.0	39.0	27.0	40.0
11	36.05564639622103	38.0	35.0	39.0	30.0	40.0
12	36.17262970749306	38.0	35.0	39.0	30.0	40.0
13	36.22278348776246	38.0	35.0	39.0	30.0	40.0
14	36.10623815827039	38.0	35.0	39.0	30.0	40.0
15	36.29552739494926	38.0	35.0	39.0	30.0	40.0
16	35.84258610397363	38.0	35.0	39.0	29.0	40.0
17	35.2126287990864	38.0	33.0	39.0	28.0	40.0
18	35.43613252355369	38.0	34.0	39.0	28.0	40.0
19	35.42238313997249	38.0	33.0	39.0	28.0	40.0
20	35.253185911910506	38.0	33.0	39.0	28.0	40.0
21	35.152560409042536	38.0	33.0	39.0	28.0	40.0
22	35.078547976848604	38.0	33.0	39.0	28.0	40.0
23	34.90886734667394	38.0	33.0	39.0	27.0	40.0
24	34.74623660100184	38.0	33.0	39.0	27.0	40.0
25	34.62380284980145	38.0	33.0	39.0	27.0	40.0
26	34.129720470295105	37.0	33.0	39.0	25.0	40.0
27	33.93014028394197	37.0	33.0	39.0	25.0	40.0
28	33.666332502790105	36.0	32.0	39.0	24.0	40.0
29	33.39959186586727	36.0	32.0	39.0	23.0	40.0
30	33.37775441874951	36.0	32.0	39.0	23.0	40.0
31	32.99619442497859	36.0	31.0	38.0	22.0	40.0
32	32.77385216330556	36.0	31.0	38.0	22.0	40.0
33	32.57989760959278	36.0	30.0	38.0	21.0	40.0
34	32.20591956707934	35.0	30.0	38.0	20.0	40.0
35	31.809776402190558	35.0	29.0	38.0	18.0	40.0
36	33.071978250149236	36.0	32.0	39.0	22.0	40.0
37	32.8810993018246	36.0	31.0	39.0	21.0	40.0
38	32.73746074385528	36.0	31.0	39.0	21.0	40.0
39	32.397745853772484	36.0	31.0	39.0	19.0	40.0
40	32.48670157543668	36.0	31.0	39.0	20.0	40.0
41	32.54233175011031	36.0	31.0	39.0	19.0	40.0
42	32.377806327701215	36.0	31.0	39.0	18.0	40.0
43	32.24436463443121	36.0	31.0	39.0	18.0	40.0
44	31.90042240909445	36.0	30.0	39.0	15.0	40.0
45	31.843948713955722	36.0	30.0	39.0	16.0	40.0
46	31.383250279010614	36.0	30.0	39.0	9.0	40.0
47	31.392421941913884	36.0	30.0	38.0	11.0	40.0
48	31.294213449609384	35.0	30.0	38.0	10.0	40.0
49	31.18137636585429	35.0	30.0	38.0	8.0	40.0
50	30.58628889927068	35.0	29.0	38.0	2.0	40.0
51	30.657316566741933	35.0	29.0	38.0	2.0	40.0
52	30.52664875807833	35.0	29.0	38.0	2.0	40.0
53	30.29206571673285	35.0	29.0	38.0	2.0	40.0
54	30.069311427755718	35.0	29.0	38.0	2.0	40.0
55	29.836947494095355	35.0	29.0	38.0	2.0	40.0
56	29.456591139141945	35.0	28.0	38.0	2.0	40.0
57	29.288133613641673	35.0	28.0	38.0	2.0	40.0
58	28.99947442186405	35.0	27.0	38.0	2.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-7.954461604455194
1	2	-14.924492139263052
1	3	-12.765422651638982
1	4	-16.4280039570163
1	5	-17.565850804534488
1	6	-12.815379828678402
1	7	-11.75839441278806
1	8	-12.802855798620506
1	9	-10.310490596512146
1	10	-13.15585736594235
1	11	-8.82420244134012
1	12	-8.247286353939401
1	13	-8.264805999680142
1	14	-7.616338829612587
1	15	-7.059238433823179
1	16	-6.816914526290304
1	17	-7.310764304404419
1	18	-10.411692501289817
1	19	-9.405576607694552
1	20	-8.459554595867182
1	21	-10.47005413296381
1	22	-9.363301126097241
1	23	-10.299040443398315
1	24	-9.757772600079186
1	25	-15.104856159509001
1	26	-14.318933782536742
1	27	-13.068973770854658
1	28	-13.173260942823084
1	29	-10.36032991279082
1	30	-15.527265588573876
1	31	-14.664439236749793
1	32	-10.373519485590169
1	33	-9.826588634980126
1	34	-13.468089010986077
1	35	-8.432143860976094
1	36	-7.912952707900914
1	37	-7.347226477515655
1	38	-7.5970758478137945
1	39	-5.646012792304834
1	40	-9.245593666128226
1	41	-12.623144025321949
1	42	-12.376868958895237
1	43	-12.519002489078193
1	44	-14.095317948232061
1	45	-10.027769241983734
1	46	-13.437417498904768
1	47	-16.117933178576138
1	48	-17.585939134866194
1	49	-15.378010369315744
1	50	-15.906380781551073
1	51	-15.504230635448195
1	52	-13.448796216115198
1	53	-13.106054071401086
1	54	-12.950005451438685
1	55	-12.850547280352188
1	56	-15.934760028964517
1	57	-15.316954327156914
1	58	-14.81819658583743
2	1	-3.348400998394588
2	2	-7.550754765525678
2	3	-4.3714832576995875
2	4	-8.862347391359734
2	5	-10.081002319686004
2	6	-4.148713162011735
2	7	-3.960414614808265
2	8	-3.4998254955902013
2	9	-5.179177465199015
2	10	-5.044746254831239
2	11	-4.591879209016888
2	12	-4.429104535757585
2	13	-3.9617756966498376
2	14	-4.808258021531781
2	15	-5.756208130792874
2	16	-7.352268061643841
2	17	-7.795612789252903
2	18	-7.502601592198911
2	19	-6.375273577391525
2	20	-7.540362676675262
2	21	-7.48015514306482
2	22	-6.25219001498613
2	23	-7.591969736327609
2	24	-6.040600882907469
2	25	-7.024048078700922
2	26	-6.9754994391024
2	27	-6.8164485183294055
2	28	-7.233867003429147
2	29	-7.178511730972637
2	30	-5.618174679482966
2	31	-9.078580650891205
2	32	-8.333115445186127
2	33	-8.08921489760639
2	34	-10.468089010986077
2	35	-11.270527699359931
2	36	-11.407902202850408
2	37	-11.327024457313637
2	38	-11.061722312460258
2	39	-11.706618852910896
2	40	-12.104179524714084
2	41	-7.75445715663508
2	42	-8.811212393238673
2	43	-7.4280933981691035
2	44	-8.903398756312871
2	45	-8.694435908650402
2	46	-9.629336690823962
2	47	-8.744195804838764
2	48	-10.515232064159125
2	49	-10.04467703598241
2	50	-9.825572700742992
2	51	-9.958776089993648
2	52	-10.428594195913178
2	53	-11.227266192613207
2	54	-11.293439794873027
2	55	-10.931355361160271
2	56	-7.803446897651387
2	57	-8.367459377661966
2	58	-9.333348100988946
3	1	-3.0100171600107473
3	2	-6.396714361485273
3	3	-3.293200429416757
3	4	-8.789115068127412
3	5	-8.704739693423377
3	6	-3.593157606456181
3	7	-1.7861721905658356
3	8	-1.8861891319538415
3	9	-1.9493794854010318
3	10	-2.7391906992756816
3	11	-2.129757996895677
3	12	-1.386175242828287
3	13	-2.514805999680142
3	14	-2.25522771850148
3	15	-2.0314606560453967
3	16	-6.261358970734751
3	17	-4.116319859959976
3	18	-5.606136945734264
3	19	-6.405576607694552
3	20	-5.376221262533846
3	21	-3.914498577408253
3	22	-4.085523348319462
3	23	-5.632373776731647
3	24	-6.257772600079186
3	25	-5.715967270620112
3	26	-6.846711560314521
3	27	-4.680084881965769
3	28	-6.1454831650453094
3	29	-5.4714410239019315
3	30	-6.110598921907208
3	31	-6.664439236749793
3	32	-8.429075041145722
3	33	-7.993255301646794
3	34	-8.551422344319413
3	35	-8.154366083198315
3	36	-5.940730485678692
3	37	-5.236115366404544
3	38	-6.180409181147127
3	39	-6.59045723674928
3	40	-4.995593666128226
3	41	-6.539810691988617
3	42	-5.515757847784126
3	43	-6.2412247113004184
3	44	-5.178651281565397
3	45	-5.055547019761512
3	46	-4.326306387793657
3	47	-5.506822067465027
3	48	-5.141494690421752
3	49	-6.878010369315742
3	50	-4.628603003773296
3	51	-7.504230635448195
3	52	-9.53212954944853
3	53	-8.30049851584553
3	54	-9.394449895883131
3	55	-8.044991724796635
3	56	-8.184760028964519
3	57	-10.178065438268025
3	58	-8.81819658583743
4	1	-2.805338797437649
4	2	-5.597006759146094
4	3	-3.8355980902354716
4	4	-8.878296354677119
4	5	-7.741289401025714
4	6	-2.94695877604682
4	7	-2.474768681793911
4	8	-2.5133821144099784
4	9	-2.5444087251671164
4	10	-2.834219939041766
4	11	-4.125372031983392
4	12	-3.4051810907815074
4	13	-2.422700736522245
4	14	-2.382420700957617
4	15	-3.445203346103881
4	16	-3.9894291461733467
4	17	-4.37216781317635
4	18	-4.157306536377536
4	19	-5.809085379624378
4	20	-4.746104303469519
4	21	-4.56069740781761
4	22	-4.5328917693720925
4	23	-4.489098922930477
4	24	-4.892275524055794
4	25	-4.756902943134733
4	26	-6.272150156805747
4	27	-4.86429540828156
4	28	-5.62940129370028
4	29	-6.035768509282047
4	30	-6.3693708517317695
4	31	-5.740462628562657
4	32	-6.317963930034612
4	33	-5.104366412757905
4	34	-3.213703046073796
4	35	-5.850272515946852
4	36	-5.898332824859978
4	37	-5.7448872962291055
4	38	-6.266666491088646
4	39	-8.12846893265571
4	40	-5.862552730455711
4	41	-4.827822387895047
4	42	-5.318389426731496
4	43	-6.55409020837644
4	44	-4.846779936536155
4	45	-6.404962224439874
4	46	-6.0105169141094485
4	47	-7.834307447581985
4	48	-7.056699368784326
4	49	-7.570992825456095
4	50	-7.119831073948735
4	51	-7.504230635448195
4	52	-8.074527210267245
4	53	-7.658685650348456
4	54	-7.99678907716968
4	55	-7.023061900235231
4	56	-7.484467631303698
4	57	-7.305258420724165
4	58	-6.3445123753111154
5	1	-2.9306520806456717
5	2	-5.630841345612257
5	3	-4.074946461162789
5	4	-8.031178560190902
5	5	-9.184898423582105
5	6	-3.9582369715355448
5	7	-2.7742674286610765
5	8	-2.9219034176681262
5	9	-3.0406493266708736
5	10	-3.187603397688381
5	11	-2.5702341873718666
5	12	-3.604429211082259
5	13	-3.050520285394427
5	14	-3.19570390897767
5	15	-2.7259051004898467
5	16	-4.713739923115703
5	17	-4.953621447261561
5	18	-5.2926448822422
5	19	-5.834148036265983
5	20	-4.261141897454483
5	21	-5.835133498043174
5	22	-5.9188566816527945
5	23	-6.100627744985616
5	24	-4.5752329175395055
5	25	-5.6842212388740805
5	26	-4.430044893647853
5	27	-5.227703929584816
5	28	-6.982784752346895
5	29	-4.979377531838438
5	30	-6.027265588573876
5	31	-5.918407490718046
5	32	-7.198916310986991
5	33	-7.937699746091237
5	34	-7.8966604395575075
5	35	-7.019445448277679
5	36	-6.61136540631361
5	37	-6.736115366404544
5	38	-5.462155212893158
5	39	-5.431727078019122
5	40	-5.753530174064732
5	41	-6.940604342782265
5	42	-6.956234038260316
5	43	-7.971383441459146
5	44	-5.968333821247935
5	45	-6.6944359086504015
5	46	-5.2310682925555625
5	47	-6.824282384925343
5	48	-5.339907388834451
5	49	-5.258962750268125
5	50	-3.747650622820913
5	51	-5.766135397352958
5	52	-6.194827962146945
5	53	-7.058435023782039
5	54	-7.029370530803764
5	55	-5.842610772415682
5	56	-6.117299711504199
5	57	-6.356636866839455
5	58	-5.080101347742193
6	1	-2.8044616044551915
6	2	-4.6522699170408295
6	3	-2.8987559849723112
6	4	-7.51689284590519
6	5	-6.365850804534485
6	6	-2.6320464953450724
6	7	-2.452838857232507
6	8	-2.3861891319538415
6	9	-2.388268374289922
6	10	-3.4280795881645716
6	11	-2.363091330229011
6	12	-3.013953020606067
6	13	-3.2314726663468107
6	14	-2.6052277185014745
6	15	-2.6759051004898424
6	16	-4.7780256374014165
6	17	-5.060764304404419
6	18	-4.445025834623152
6	19	-3.988909941027888
6	20	-4.3039990403116235
6	21	-4.658943021852696
6	22	-4.552190014986127
6	23	-4.660151554509426
6	24	-5.163328155634744
6	25	-5.577078381731223
6	26	-5.396711560314518
6	27	-5.896751548632434
6	28	-5.856594276156418
6	29	-5.926996579457487
6	30	-6.027265588573876
6	31	-5.442217014527568
6	32	-4.184630596701279
6	33	-6.487699746091238
6	34	-5.218089010986077
6	35	-5.75992163875387
6	36	-3.8351749301231344
6	37	-4.352782033071211
6	38	-4.685964736702683
6	39	-4.446012792304835
6	40	-4.5511492216837794
6	41	-3.2453662475441725
6	42	-5.615757847784128
6	43	-3.6523358224115263
6	44	-4.606429059343174
6	45	-4.361102575317069
6	46	-2.2763063877936567
6	47	-4.412377623020582
6	48	-4.98038357931064
6	49	-2.961343702649078
6	50	-2.7452696704399635
6	51	-4.520897302114861
6	52	-4.287685105004087
6	53	-4.256054071401085
6	54	-4.7388943403275725
6	55	-4.944991724796633
6	56	-5.462537806742294
6	57	-5.361398771601358
6	58	-5.201529919170763
7	1	-2.367963714159835
7	2	-4.854168651218046
7	3	-2.554452187503962
7	4	-6.793686094850337
7	5	-6.481462618880478
7	6	-2.435632993235366
7	7	-1.6922903340257562
7	8	-1.7901975707724063
7	9	-2.0713907371591205
7	10	-3.158670305464149
7	11	-2.350222131916773
7	12	-2.9941217969773746
7	13	-2.9483503034776106
7	14	-2.1972108408643436
7	15	-2.3799135393084114
7	16	-3.9111480002706145
7	17	-4.4837600849951365
7	18	-3.6479794211210432
7	19	-4.654521755373878
7	20	-4.941973723855927
7	21	-3.7429092665784367
7	22	-4.193118285028323
7	23	-3.8658477570410703
7	24	-3.4905433455081614
7	25	-4.601339985106751
7	26	-4.354095526559245
7	27	-4.6104646288012106
7	28	-5.0298010272112705
7	29	-3.769612613212761
7	30	-4.780430145535902
7	31	-6.56457988372588
7	32	-6.4002424110472695
7	33	-7.515758817821197
7	34	-7.349945551070466
7	35	-6.62342374845851
7	36	-7.375681259237059
7	37	-6.47240228623577
7	38	-7.9079056649727235
7	39	-7.160780724794286
7	40	-6.910853863033992
7	41	-6.474058230666536
7	42	-7.278416075632226
7	43	-7.3755425734663795
7	44	-5.4933488905668035
7	45	-7.453929579536478
7	46	-5.376939299186063
7	47	-5.646765808674591
7	48	-6.562029148930893
7	49	-6.525689694210257
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119	56	-5.5919704308557705
119	57	-5.201115083658095
119	58	-5.839473181582111
120	1	-3.335413985407577
120	2	-10.559412774183688
120	3	-4.670184556400887
120	4	-8.876416655428997
120	5	-9.613469852153536
120	6	-4.386808400106975
120	7	-3.6195055238991714
120	8	-3.9814272271919364
120	9	-4.290649326670874
120	10	-4.259031969116954
120	11	-2.5107103778480564
120	12	-2.890143496796547
120	13	-3.9433774282515692
120	14	-2.850465813739575
120	15	-3.892571767156511
120	16	-8.02326373263951
120	17	-5.870288113928229
120	18	-7.721216310813627
120	19	-5.679386131504078
120	20	-7.93971332602591
120	21	-9.049419212328889
120	22	-7.835523348319462
120	23	-7.553008697366568
120	24	-8.134756727063316
120	25	-9.24374504839789
120	26	-7.1443306079335684
120	27	-7.858656310537196
120	28	-9.375641895204037
120	29	-8.157948960409868
120	30	-9.955837017145303
120	31	-7.716026538337093
120	32	-9.782249644320327
120	33	-9.81865212704362
120	34	-8.884755677652745
120	35	-8.805159733991964
120	36	-11.385174930123135
120	37	-10.50992489021407
120	38	-10.890726641464589
120	39	-10.098393744685787
120	40	-10.074958745493305
120	41	-11.583461485639411
120	42	-10.658614990641269
120	43	-10.614240584316288
120	44	-9.37309572600984
120	45	-10.05157876579326
120	46	-9.254877816365084
120	47	-11.407615718258675
120	48	-10.173240722167783
120	49	-9.687534178839552
120	50	-8.85479347996377
120	51	-9.361373492591053
120	52	-9.623399390718372
120	53	-9.034625499972513
120	54	-9.624608626041859
120	55	-8.735467915272825
120	56	-7.200633044837534
120	57	-8.987589247791835
120	58	-7.103910871551715
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	321.0
3	46.0
4	80.0
5	116.0
6	240.0
7	332.0
8	397.0
9	562.0
10	716.0
11	851.0
12	1121.0
13	1276.0
14	1473.0
15	1588.0
16	1627.0
17	1646.0
18	1765.0
19	1914.0
20	1957.0
21	2201.0
22	2554.0
23	2967.0
24	3630.0
25	4421.0
26	5393.0
27	6459.0
28	7532.0
29	9140.0
30	10606.0
31	12259.0
32	14325.0
33	17185.0
34	20540.0
35	25851.0
36	31827.0
37	39199.0
38	38942.0
39	35112.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	100.0	0.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	41.01480746366914	43.31895492910694	3.5130470098487154	12.153190597375204
11	24.2920815059844	19.999285832540718	27.232179087229063	28.476453574245824
12	25.026603337745595	14.307729242907941	32.98067689273015	27.684990526616314
13	18.413727322276728	15.529211762568455	37.52076358067949	28.53629733447533
14	18.073074826753874	20.151379480391395	39.244789638973245	22.530756053881493
15	21.91401282151107	20.577681746217134	34.4260167665914	23.082288665680398
16	27.250253056139535	21.82317215603831	27.962378987256354	22.964195800565808
17	26.666320429507195	21.05176029135857	25.227380434570918	27.054538844563318
18	25.558021230761245	22.11970204261725	25.284850372446726	27.037426354174777
19	25.04444703989203	24.1470710374004	24.864387863687092	25.94409405902048
20	27.20848973659366	22.418900110631313	26.370806310851275	24.001803841923753
21	26.833034856861065	20.566975524929273	29.014832982947908	23.58515663526175
22	27.4702383849396	19.307330653246765	29.072125477370143	24.150305484443493
23	28.439616911936465	20.88848659451322	26.8012406239456	23.87065586960471
24	28.164499467933247	22.472034052272313	24.941926860287055	24.421539619507385
25	27.170552067617333	25.5800522379585	22.41040865657598	24.838987037848185
26	28.024276649952128	24.73654317381497	23.048212518053322	24.190967658179577
27	25.429900082102076	21.98940136880056	23.290378480819594	29.29032006827777
28	25.59206169906127	24.697801841104145	24.15378023604565	25.556356223788935
29	28.765783187350703	21.477810097989828	23.984919641841493	25.771487072817976
30	24.46770505858817	21.864688612330795	25.07404713034893	28.593559198732105
31	26.06384877205164	23.90289327929863	23.10502697949688	26.928230969152843
32	29.36803577107034	22.418895328085352	24.955383507148373	23.257685393695933
33	29.23603133498186	22.240178611992707	24.783387527015712	23.74040252600972
34	27.15716849425761	24.914407733971125	26.613596757391768	21.3148270143795
35	26.779090032811787	26.61162335575959	26.407807322448	20.20147928898063
36	28.668733665568237	27.07530477411814	23.683911497816666	20.572050062496956
37	28.95693730195834	26.1940938133084	23.73805256869773	21.11091631603553
38	26.405990232751453	28.049992206982544	23.079345906068163	22.464671654197836
39	27.52793287011459	25.545495316897515	23.262442233069656	23.66412957991823
40	24.806956702450304	27.327072820672683	24.180905436385007	23.68506504049201
41	24.85825068682657	26.465067643486677	26.143249615182278	22.53343205450448
42	25.143872275195513	25.34750188365507	25.80574969471797	23.702876146431446
43	26.51273523317072	24.65328075976173	25.324308347895002	23.50967565917255
44	26.130023909825802	23.04419251476066	25.21526975813666	25.610513817276882
45	25.508533487522982	23.78949214867174	26.2790486152167	24.422925748588582
46	25.77449871999792	26.039920470936806	25.64519901758216	22.540381791483114
47	24.69435428586281	24.466927233089987	26.33800208583097	24.500716395216234
48	26.273689272126504	25.117370129342692	24.97896272422162	23.629977874309184
49	25.27734270915247	25.950309025092448	25.161336444164267	23.611011821590814
50	21.463944057398358	25.129653218259335	27.040007278777168	26.366395445565143
51	21.8083550540472	25.615384115387364	27.282949943775307	25.29331088679012
52	24.327618824722563	25.925275811180676	26.155416431000074	23.59168893309669
53	24.010568667086986	25.91728360556643	26.80288070925766	23.26926701808893
54	24.123294527537816	25.55754892975306	25.439246504553342	24.87991003815578
55	23.82138189762228	25.811265059849287	25.466018218110182	24.90133482441825
56	23.795517842734064	26.171946172466747	24.32487831541685	25.70765766938234
57	25.373959417273674	25.660119667013525	24.772372528616025	24.193548387096776
58	23.974552042439285	26.516745757860605	25.72930320603934	23.779398993660777
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	260.0
10	520.0
11	874.0
12	1228.0
13	1431.0
14	1634.0
15	1372.0
16	947.0
17	784.0
18	782.5
19	781.0
20	692.5
21	604.0
22	654.5
23	903.5
24	1102.0
25	1380.5
26	1659.0
27	1930.0
28	2673.0
29	3145.0
30	3774.0
31	4403.0
32	4589.0
33	4775.0
34	5697.5
35	6594.5
36	6569.0
37	7058.5
38	7548.0
39	7726.5
40	7905.0
41	7799.5
42	8285.0
43	8876.0
44	9432.0
45	9988.0
46	10829.0
47	11841.0
48	12012.0
49	12229.0
50	12446.0
51	12735.5
52	13025.0
53	13791.5
54	15727.0
55	16896.0
56	17150.5
57	17405.0
58	19870.5
59	25729.0
60	29122.0
61	25378.5
62	21635.0
63	19415.5
64	17196.0
65	15257.0
66	11041.5
67	8765.0
68	7634.5
69	6504.0
70	5330.0
71	4156.0
72	3718.5
73	2608.0
74	1935.0
75	1419.0
76	903.0
77	731.0
78	436.0
79	313.0
80	255.0
81	197.0
82	140.0
83	83.0
84	64.0
85	26.5
86	8.0
87	8.5
88	9.0
89	5.0
90	1.0
91	0.5
92	1.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.945067351864829
11	0.0587220016091775
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.05126008980248644
18	0.0
19	0.0
20	3.244309481170028E-4
21	0.0
22	0.010706221287861091
23	0.0
24	0.0
25	0.008759635599159075
26	0.037958420929689325
27	0.02563004490124322
28	0.050611227906252436
29	0.1774637286200005
30	0.1038179033974409
31	0.011355083184095097
32	0.016221547405850138
33	0.025305613953126218
34	0.027576630589945236
35	0.036011835240987305
36	0.0704015157413896
37	0.0700770847932726
38	0.08564977030288873
39	0.1719484025020115
40	0.08759635599159074
41	0.09538269874639882
42	0.1038179033974409
43	0.11225310804848297
44	0.2683043940927613
45	0.12523034597316307
46	0.13626099820914117
47	0.14307404811959823
48	0.14437177191206624
49	0.15897116457733138
50	0.15832230268109734
51	0.1745438500869475
52	0.19271198318149965
53	0.1719484025020115
54	0.17713929767188352
55	0.20309377352124375
56	0.2854992343429624
57	0.230994835059306
58	0.2533805704793792
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
58	308232.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.00813085787964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.94123205579983	23.10554310399208
2	11.065661448714316	7.526449251537411
3	5.708567181438977	5.824130991521218
4	3.711456811629568	5.048788356930685
5	2.654620963982898	4.51393485606005
6	1.8666738058724823	3.8089252235452555
7	1.372069505070936	3.2663063492190982
8	0.9482818506783545	2.579943461441741
9	0.7588247053018393	2.3225588888487216
>10	3.5248951458407394	20.804009114971347
>50	0.26922820029156325	6.331846613210932
>100	0.15854549572725393	9.925386596083483
>500	0.017948546686104218	3.9835155401343627
>1k	0.0019942829651226905	0.9586616525036047
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGGATGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	1777	0.576513794803914	No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	1050	0.3406524955228529	No Hit
TGGATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	949	0.30788496976303564	No Hit
TGGATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	772	0.25046069194632614	No Hit
TGGATGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG	754	0.2446209348802201	No Hit
TGGATGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT	752	0.24397207298398607	No Hit
TGGATGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	733	0.23780788496976304	No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA	720	0.233590282644242	No Hit
TGGATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGC	680	0.2206130447195619	No Hit
TGGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	676	0.21931532092709388	No Hit
TGGATGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC	673	0.21834202808274286	No Hit
TGGATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT	661	0.21444885670533886	No Hit
TGGATGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA	622	0.20179604972877574	No Hit
TGGATGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT	563	0.18265462378987254	No Hit
TGGATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	550	0.17843702146435153	No Hit
TGGATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG	540	0.17519271198318148	No Hit
TGGATGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA	538	0.1745438500869475	No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	532	0.17259726439824546	No Hit
TGGATGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC	519	0.16837966207272442	No Hit
TGGATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTC	513	0.16643307638402244	No Hit
TGGATGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG	486	0.15767344078486337	No Hit
TGGATGGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGGGC	472	0.1531314075112253	No Hit
TGGATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAAG	467	0.15150925277064028	No Hit
TGGATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	465	0.15086039087440628	No Hit
TGGATGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGGT	450	0.14599392665265123	No Hit
TGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	442	0.14339847906771525	No Hit
TGGATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	433	0.1404786005346622	No Hit
TGGATGGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG	420	0.13626099820914117	No Hit
TGGATGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGC	418	0.13561213631290717	No Hit
TGGATGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG	413	0.13398998157232214	No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC	385	0.12490591502504608	No Hit
TGGATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCG	384	0.12458148407692907	No Hit
TGGATGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA	362	0.117444003218355	No Hit
TGGATGGGGAAACAGTAAGCTCCTGGGTAAAACCTTGCTCTCAGATCAATAGATTATC	356	0.115497417529653	No Hit
TGGATGGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACT	320	0.1038179033974409	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0035687404292870303	0.0	3.244309481170028E-4	0.0	0.0
11	0.0061641880142230526	0.0	3.244309481170028E-4	0.0	0.0
12	0.0061641880142230526	0.0	3.244309481170028E-4	0.0	0.0
13	0.0061641880142230526	0.0	3.244309481170028E-4	0.0	0.0
14	0.009084066547276077	0.0	3.244309481170028E-4	0.0	0.0
15	0.00940849749539308	0.0	3.244309481170028E-4	0.0	0.0
16	0.00940849749539308	0.0	3.244309481170028E-4	0.0	0.0
17	0.00940849749539308	0.0	3.244309481170028E-4	0.0	0.0
18	0.00940849749539308	0.0	3.244309481170028E-4	0.0	0.0
19	0.010057359391627087	0.0	3.244309481170028E-4	0.0	0.0
20	0.012652806976563109	0.0	3.244309481170028E-4	0.0	0.0
21	0.012652806976563109	0.0	3.244309481170028E-4	0.0	0.0
22	0.013301668872797113	0.0	3.244309481170028E-4	0.0	0.0
23	0.014274961717148121	0.0	3.244309481170028E-4	0.0	0.0
24	0.015572685509616133	0.0	3.244309481170028E-4	0.0	0.0
25	0.015897116457733135	0.0	3.244309481170028E-4	0.0	0.0
26	0.016870409302084145	0.0	3.244309481170028E-4	0.0	0.0
27	0.017194840250201147	0.0	3.244309481170028E-4	0.0	0.0
28	0.022385735420073192	0.0	3.244309481170028E-4	0.0	0.0
29	0.023034597316307196	0.0	3.244309481170028E-4	0.0	0.0
30	0.025305613953126218	0.0	3.244309481170028E-4	0.0	0.0
31	0.02563004490124322	0.0	3.244309481170028E-4	0.0	0.0
32	0.027252199641828234	0.0	3.244309481170028E-4	0.0	0.0
33	0.027576630589945236	0.0	3.244309481170028E-4	0.0	0.0
34	0.02790106153806224	0.0	3.244309481170028E-4	0.0	0.0
35	0.033091956707934286	0.0	3.244309481170028E-4	0.0	0.0
36	0.03374081860416829	0.0	3.244309481170028E-4	0.0	0.0
37	0.03406524955228529	0.0	3.244309481170028E-4	0.0	0.0
38	0.0353629733447533	0.0	3.244309481170028E-4	0.0	3.244309481170028E-4
39	0.03698512808533832	0.0	3.244309481170028E-4	0.0	3.244309481170028E-4
40	0.03860728282592333	0.0	3.244309481170028E-4	0.0	3.244309481170028E-4
41	0.04120273041085935	0.0	3.244309481170028E-4	0.0	3.244309481170028E-4
42	0.04412260894391238	0.0	3.244309481170028E-4	0.0	3.244309481170028E-4
43	0.04574476368449739	0.0	3.244309481170028E-4	0.0	3.244309481170028E-4
44	0.046393625580731394	0.0	3.244309481170028E-4	0.0	3.244309481170028E-4
45	0.048664642217550416	0.0	3.244309481170028E-4	0.0	3.244309481170028E-4
46	0.05158452075060344	0.0	3.244309481170028E-4	0.0	3.244309481170028E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACTAG	30	7.5539356E-7	52.41105	9
GACTAAT	20	3.3123686E-4	52.41105	9
AGCTTAC	15	0.0071956026	52.03565	29
ATACCCG	15	0.007204872	52.01871	27
TGCGGAT	25	1.6504133E-5	52.010246	38
GTGTAGG	25	1.6504133E-5	52.010246	39
ATCTCGC	15	0.0072234385	51.984875	41
CAGATTA	15	0.00724204	51.95108	50
GCGCTTA	15	0.00724204	51.95108	50
CGAGTGG	15	0.007246696	51.94264	30
CATACGT	15	0.0072560147	51.925762	33
ATACGTG	15	0.0072560147	51.925762	34
CCGGATT	15	0.007260677	51.91733	45
CGGATTA	15	0.007260677	51.91733	46
TCGCAAC	15	0.0072700093	51.90047	15
TCGTGCA	20	3.47642E-4	51.90047	12
TCCGTAA	15	0.0072700093	51.90047	12
GCGTCCA	15	0.0072700093	51.90047	31
CACGCGA	15	0.007284024	51.875202	19
ATGGGGG	12610	0.0	51.321484	4
>>END_MODULE