FastQCFastQC Report
Tue 31 May 2016
SRR212823_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212823_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462616
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC27970.6046051152575787No Hit
TGGATGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC24740.5347847891123523No Hit
TGGATGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC19010.4109239628547218No Hit
TGGATGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA15480.33461877669600704No Hit
TGGATGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC14260.3082470126411538No Hit
TGGATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT14060.30392377263216147No Hit
TGGATGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG11840.2559358085323465No Hit
TGGATGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA11830.2557196465318968No Hit
TGGATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC11770.25442267452919914No Hit
TGGATGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG10780.2330226364846871No Hit
TGGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10780.2330226364846871No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA10770.23280647448423747No Hit
TGGATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT10460.22610545247029934No Hit
TGGATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA10000.21616200044961695No Hit
TGGATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG9460.20448925242533764No Hit
TGGATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGA9140.1975720684109499No Hit
TGGATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC8760.18935791239386446No Hit
TGGATGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA8560.18503467238487212No Hit
TGGATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8290.17919829837273246No Hit
TGGATGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC8040.17379424836149204No Hit
TGGATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCC7010.1515295623151815No Hit
TGGATGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC6820.14742248430663876No Hit
TGGATGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG6730.1454770263025922No Hit
TGGATGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTC6670.1441800542998945No Hit
TGGATGGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCT6610.14288308229719682No Hit
TGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6600.1426669202967472No Hit
TGGATGGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGG6590.14245075829629758No Hit
TGGATGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC6000.1296972002697702No Hit
TGGATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAG5670.12256385425493282No Hit
TGGATGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG5530.11953758624863818No Hit
TGGATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCA5410.11694364224324279No Hit
TGGATGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT5400.11672748024279317No Hit
TGGATGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA5370.11607899424144431No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC5120.1106749442302039No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT5020.10851332422570771No Hit
TGGATGGGGAAACAGTAAGCTCCTGGGTAAAACCTTGCTCTCAGATCAATAGATTA5020.10851332422570771No Hit
TGGATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGC4900.1059193802203123No Hit
TGGATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACAC4880.10548705621941307No Hit
TGGATGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA4640.10029916820862228No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCG204.0868213E-450.23203748
ACTACGA301.0136118E-650.23203750
GTGCCGA150.00830350650.18831644
GTCGCAA150.00830707850.18285842
GTTGTCG150.0083536150.1119939
TATCGAA150.0083536150.1119939
ATGTCGC150.00835719750.10655238
CCTATCG150.00835719750.10655237
TATGTCG150.00835719750.10655237
CGAATAC252.059416E-550.10654437
TCTCGAG150.00837874650.07391733
CCGTAGA150.00840033650.0413330
TCGAACG150.00841835950.01420228
ACACGCG150.00842557750.0033626
ATTCGTC252.084687E-550.0033626
GTATACG355.277434E-850.00335726
CGGTATC150.00843641249.98710625
AGATTCG252.0927206E-549.97086324
AACGCGA150.00846535449.94381323
GTCGTCC150.00850890849.87901319
Produced by FastQC (version 0.11.5)