FastQCFastQC Report
Tue 31 May 2016
SRR212822_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212822_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439115
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC34270.7804333716680141No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC26300.5989319426573905No Hit
TGGATGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC25360.5775252496498638No Hit
TGGATGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA19600.44635232228459515No Hit
TGGATGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC14500.3302096261799301No Hit
TGGATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT12690.2889903556016078No Hit
TGGATGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA12230.27851473987452036No Hit
TGGATGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA11920.2714550857975701No Hit
TGGATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC11790.2684945857007845No Hit
TGGATGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG11240.25596939298361476No Hit
TGGATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC11120.253236623663505No Hit
TGGATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT10360.23592908463614315No Hit
TGGATGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG10050.22886943055919293No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA10040.22864169978251708No Hit
TGGATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA9960.2268198535691106No Hit
TGGATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG9690.22067112259886362No Hit
TGGATGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT9030.2056408913382599No Hit
TGGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8880.2022249296881227No Hit
TGGATGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC7840.17854092891383805No Hit
TGGATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGA7830.17831319813716226No Hit
TGGATGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTC7680.17489723648702504No Hit
TGGATGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG7210.1641938899832618No Hit
TGGATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7070.1610056591098004No Hit
TGGATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCC6930.15781742823633899No Hit
TGGATGGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGG6900.15713423590631154No Hit
TGGATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACAC6670.15189642804276782No Hit
TGGATGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG6170.14050988920897714No Hit
TGGATGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC5710.1300342734818897No Hit
TGGATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAG5610.12775696571513157No Hit
TGGATGGGGATCTTAATAACTACTATAGGAACAAAGGAAGTGGCTTTAATGACCCT5560.1266183118317525No Hit
TGGATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCA5470.12456873484167018No Hit
TGGATGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5230.11910319620145064No Hit
TGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5140.11705361921136832No Hit
TGGATGGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT5010.11409311911458274No Hit
TGGATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGC4830.10999396513441809No Hit
TGGATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGC4630.10543934960090182No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC4600.10475615727087437No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT4580.10430069571752275No Hit
TGGATGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAG4580.10430069571752275No Hit
TGGATGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA4580.10430069571752275No Hit
TGGATGGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCA4480.10202338795076461No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4450.10134019562073716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGAA204.0702272E-450.2728949
ACACGAT150.0082743950.23250245
CGAATAC204.116574E-450.15767737
ACGAATA204.116574E-450.15767736
CTCGTAC150.00832329450.15767336
CGTACAA150.00832329450.15767338
TCGTACA150.00832329450.15767337
CGATACT150.00832329450.15767338
CACGAAT204.128226E-450.12895235
AATTGCG204.1916006E-449.9744326
TATACGA252.0916597E-549.97442627
AATAGCG204.1963253E-449.96302425
GCGTCGA150.00845221249.96301725
CCGAACT150.00846365849.94591524
CGATGAA204.21764E-449.91174723
CAACGCG301.0600415E-649.90605522
ACGCATT150.00850955849.8776221
CGCATAT150.00852489849.85489719
CGGAATT150.00853257749.84354416
ATAGGCC150.00853257749.84354414
Produced by FastQC (version 0.11.5)