FastQCFastQC Report
Tue 31 May 2016
SRR212821_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212821_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444049
Sequences flagged as poor quality0
Sequence length58
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT19620.44184312992485064No Hit
ACTTATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16850.3794626268722596No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA13340.30041729628937347No Hit
ACTTATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13170.2965888899648462No Hit
ACTTATGGGACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAG11370.2560528229992636No Hit
ACTTATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC10760.24231560030537172No Hit
ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG10200.2297043794716349No Hit
ACTTATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA9020.2031307355719752No Hit
ACTTATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT8760.1972755258991688No Hit
ACTTATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA7240.16304506935045457No Hit
ACTTATGGGGGGCTGGAGAGATGGCTCAGCGAGTAAGAGCACCTCACTGCTCTTCCAG7150.16101826600217545No Hit
ACTTATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG6630.1493078466565627No Hit
ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6570.1479566444243766No Hit
ACTTATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC6530.1470558429362525No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA6320.14232663512360122No Hit
ACTTATGGGAGTCTTAAGCATATAAAAGAAACATTTGTAAAGATGTATAAGAGTAAGA6220.14007463140329107No Hit
ACTTATGGGAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCA6170.138948629543136No Hit
ACTTATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA6130.13804782805501195No Hit
ACTTATGGGGTGTGTTCAAACACCCCTCCCACAGGACTCACTTTGTCCCAACAATTCA6050.13624622507876383No Hit
ACTTATGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCG5990.13489502284657773No Hit
ACTTATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5920.13331862024236063No Hit
ACTTATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT5820.13106661652205048No Hit
ACTTATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5660.12746341056955426No Hit
ACTTATGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG5530.12453580573315107No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCAA5270.1186805960603447No Hit
ACTTATGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4760.10719537708676295No Hit
ACTTATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT4750.10697017671473194No Hit
ACTTATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4740.10674497634270091No Hit
ACTTATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG4610.10381737150629772No Hit
ACTTATGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCAT4500.10134016741395657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCA307.9186793E-752.06192452
TCGGCTA150.007186549752.0560549
CCTCGAT203.4289906E-452.05018248
ACGTCAG150.00719937952.03257846
GTTGCCG150.007215439352.00326537
GCCGATA307.986728E-751.99740625
TAGCGCG150.00722187151.99155422
AACGCGG150.00722187151.99155423
TTAAGCG150.00722508951.98569521
TACGTCT203.4500414E-451.98569521
CCGTAAG251.6573393E-551.97984315
CACGGCG150.00722830751.9798412
CCGTGAC150.00722830751.9798417
ATACTCG203.4519602E-451.9798415
GATAGCG150.00722830751.979849
ACTTATG451500.051.1163941
TTATGGG451700.051.0937583
CTTATGG452250.051.031622
TATGGGA208850.050.8847434
ATGGGAG59000.050.878575
Produced by FastQC (version 0.11.5)