FastQCFastQC Report
Tue 31 May 2016
SRR212820_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212820_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446007
Sequences flagged as poor quality0
Sequence length58
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT19500.43721286885631844No Hit
ACTTATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17210.38586838323165334No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA13410.3006679267365759No Hit
ACTTATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12420.278470965702332No Hit
ACTTATGGGACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAG11050.24775395901858044No Hit
ACTTATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC10490.23519810227193744No Hit
ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG9930.22264224552529444No Hit
ACTTATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA8410.1885620629272635No Hit
ACTTATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT8370.18766521601678898No Hit
ACTTATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA7450.1670377370758755No Hit
ACTTATGGGGGGCTGGAGAGATGGCTCAGCGAGTAAGAGCACCTCACTGCTCTTCCAG6890.1544818803292325No Hit
ACTTATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG6420.14394392913115714No Hit
ACTTATGGGGTGTGTTCAAACACCCCTCCCACAGGACTCACTTTGTCCCAACAATTCA6380.14304708222068263No Hit
ACTTATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC6370.142822870493064No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA6330.14192602358258952No Hit
ACTTATGGGAGTCTTAAGCATATAAAAGAAACATTTGTAAAGATGTATAAGAGTAAGA6290.141029176672115No Hit
ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6240.1399081180340219No Hit
ACTTATGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCG6170.1383386359406915No Hit
ACTTATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA5900.13228491929498865No Hit
ACTTATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5740.12869753165309064No Hit
ACTTATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT5720.1282491081978534No Hit
ACTTATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5680.12735226128737892No Hit
ACTTATGGGAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCA5420.12152275636929465No Hit
ACTTATGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG4940.11076059344360066No Hit
ACTTATGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT4790.10739741752932128No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCAA4770.10694899407408404No Hit
ACTTATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG4560.10224054779409292No Hit
ACTTATGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCAT4560.10224054779409292No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGA203.4045847E-452.12557627
CGGGCGA203.4045847E-452.12557627
CCTATCG150.00715509852.11384626
GCGGATA150.007199767552.031944
ACGTACT150.007209366752.0143741
CTACGTA150.00721256852.00853339
TACGTAC150.00721256852.00853340
CCTCGCG203.4463994E-451.9968637
ACGCGTT401.893568E-951.99102435
CGTATAA251.6552409E-551.9910233
CTTCGAC150.00722538651.98518834
CTCGACA150.00722538651.98518834
GATCGTA150.00724143251.95603618
ATCGTAG150.00725107251.9385619
TCGTCAA203.4674592E-451.93273511
TATGGGA209600.051.0159874
ACTTATG454150.051.000771
TTATGGG454250.050.9895443
CTTATGG454650.050.9446832
ATGGGAG62800.050.8163455
Produced by FastQC (version 0.11.5)