FastQCFastQC Report
Tue 31 May 2016
SRR212819_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212819_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441303
Sequences flagged as poor quality0
Sequence length58
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT17360.3933805118025484No Hit
ACTTATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15060.3412621260222568No Hit
ACTTATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12110.2744146312171003No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA11580.2624047423199027No Hit
ACTTATGGGACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAG10560.23929137123472988No Hit
ACTTATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC9690.21957702530914133No Hit
ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG8770.1987296709970247No Hit
ACTTATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT7400.1676852412061554No Hit
ACTTATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA7090.16066058920968135No Hit
ACTTATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA6480.1468378868940388No Hit
ACTTATGGGGGGCTGGAGAGATGGCTCAGCGAGTAAGAGCACCTCACTGCTCTTCCAG6330.14343886173445458No Hit
ACTTATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG6290.1425324550252321No Hit
ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6030.13664081141528608No Hit
ACTTATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC5970.1352812013514524No Hit
ACTTATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA5670.12848315103228394No Hit
ACTTATGGGGTGTGTTCAAACACCCCTCCCACAGGACTCACTTTGTCCCAACAATTCA5540.12553732922731092No Hit
ACTTATGGGAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCA5510.12485752419539409No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA5410.12259150742233793No Hit
ACTTATGGGAGTCTTAAGCATATAAAAGAAACATTTGTAAAGATGTATAAGAGTAAGA5380.1219117023904211No Hit
ACTTATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG5110.11579345710316948No Hit
ACTTATGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCG5050.11443384703933579No Hit
ACTTATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT4970.11262103362089088No Hit
ACTTATGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG4910.11126142355705718No Hit
ACTTATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4790.10854220342938978No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCA150.0070101752.38408710
GACTTAC203.3223868E-452.3840839
ATCGAGG203.3223868E-452.38408310
CTACGTA150.007186364452.05631639
GCCGATA600.052.02080525
CACGTAT150.00720578352.02080524
GACGGTC203.438528E-452.020825
ACGGTAG203.446255E-451.99715841
TGCGACA203.4559332E-451.96763234
CGTTCTT150.00723823451.9617348
ACACGTA150.007247990551.94403530
GACGCGA150.007270793451.9027911
ATTACGC150.00728384851.8792522
CGTAAGA203.4850975E-451.8792520
GCGTTGA150.0073001951.84985717
ACGATGT150.0073001951.84985712
CCGTAAG203.4948622E-451.84985719
CCCGTAA203.496818E-451.8439818
ATGGGAT65400.051.4229135
TATGGGA205700.051.2744064
Produced by FastQC (version 0.11.5)