FastQCFastQC Report
Tue 31 May 2016
SRR212818_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212818_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422341
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT19920.4716567891822011No Hit
ACTTATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17160.4063067521268359No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC14370.34024638858173845No Hit
ACTTATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12420.29407516674914347No Hit
ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC11130.26353112769065756No Hit
ACTTATGGGACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTC9750.23085610916297494No Hit
ACTTATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC8220.1946294581866312No Hit
ACTTATGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC7460.17663452044674802No Hit
ACTTATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA7230.17118868402546755No Hit
ACTTATGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTC6740.1595866846931745No Hit
ACTTATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA6700.15863958270686485No Hit
ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6620.15674537873424554No Hit
ACTTATGGGCTGTGCTGTAAGACAGCTTGACACAACTCGATGTCATTCTTTTTCCC6350.15035244032665546No Hit
ACTTATGGGAGTCTTAAGCATATAAAAGAAACATTTGTAAAGATGTATAAGAGTAA5750.13614591053201086No Hit
ACTTATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC5660.13401493106281417No Hit
ACTTATGGGGGGCTGGAGAGATGGCTCAGCGAGTAAGAGCACCTCACTGCTCTTCC5530.13093684960730784No Hit
ACTTATGGGGTGTGTTCAAACACCCCTCCCACAGGACTCACTTTGTCCCAACAATT5450.12904264563468856No Hit
ACTTATGGGGAGGGTCCAGGCCATCCTCAGCTGCCGAGCCTCATTGTGACCTTTGT5360.12691166616549185No Hit
ACTTATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG5320.12596456417918223No Hit
ACTTATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5270.12478068669629516No Hit
ACTTATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA5140.12170260524078882No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT5070.12004517676474696No Hit
ACTTATGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT5060.11980840126816956No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC4970.11767742179897286No Hit
ACTTATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4950.11720387080581805No Hit
ACTTATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC4870.11530966683319875No Hit
ACTTATGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4450.10536509597694754No Hit
ACTTATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4420.1046547694872153No Hit
ACTTATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG4260.10086636154197674No Hit
ACTTATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4230.10015603505224452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCTA252.0213742E-550.2634149
TGGTTCG204.0834484E-450.23940347
TTGCTCG150.00828529950.2154243
CGCGCAA150.00830877750.1794941
GCGTTCG150.00830877750.1794941
CGTTGTA301.0224194E-650.16752239
TCGCGCA150.00831661350.16752240
GTACGTA150.00831661350.16752239
TACGTAG150.00831661350.16752240
TACGTAC204.1124038E-450.16752240
AACATAT252.0472271E-550.1555638
ACCCACG550.050.14958236
CGCGTTT252.0486714E-550.1495836
GCGACAG252.0588048E-550.10777735
CGTCTAA204.1366578E-450.10777734
TGCGACA252.0588048E-550.10777734
TCGTCAC150.0083558850.10777734
ACTCGAT355.1899406E-850.10777735
CGATCGG150.0083558850.10777734
ACGCGTT252.0588048E-550.10777735
Produced by FastQC (version 0.11.5)