FastQCFastQC Report
Tue 31 May 2016
SRR212817_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212817_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383668
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT18010.4694162661467727No Hit
ACTTATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13850.36098918856928386No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC12870.3354462712553562No Hit
ACTTATGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC11720.3054724397135023No Hit
ACTTATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10800.2814933744800192No Hit
ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC9740.25386532105883214No Hit
ACTTATGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT8580.22363084750357082No Hit
ACTTATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC8290.2160722291147555No Hit
ACTTATGGGACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTC8060.21007746280638467No Hit
ACTTATGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTC7370.19209316388127234No Hit
ACTTATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA7030.1832313354254199No Hit
ACTTATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA6380.16628960455393724No Hit
ACTTATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA6320.1647257524734927No Hit
ACTTATGGGCTGTGCTGTAAGACAGCTTGACACAACTCGATGTCATTCTTTTTCCC5910.15403942992378827No Hit
ACTTATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC5780.15065108374949177No Hit
ACTTATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG5560.1449169594545284No Hit
ACTTATGGGGGGCTGGAGAGATGGCTCAGCGAGTAAGAGCACCTCACTGCTCTTCC5540.14439567542771356No Hit
ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5330.1389221931461576No Hit
ACTTATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC5010.13058164871712No Hit
ACTTATGGGGAGGGTCCAGGCCATCCTCAGCTGCCGAGCCTCATTGTGACCTTTGT4900.12771458656963836No Hit
ACTTATGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT4640.12093789422104528No Hit
ACTTATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC4610.120155968180823No Hit
ACTTATGGGAGTCTTAAGCATATAAAAGAAACATTTGTAAAGATGTATAAGAGTAA4530.11807083207356361No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT4510.11754954804674876No Hit
ACTTATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4460.11624633797971164No Hit
ACTTATGGGGTGTGTTCAAACACCCCTCCCACAGGACTCACTTTGTCCCAACAATT4450.1159856959663042No Hit
ACTTATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG4440.11572505395289678No Hit
ACTTATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4150.10816643556408145No Hit
ACTTATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4120.10738450952385917No Hit
ACTTATGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCA4100.10686322549704431No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC4020.1047780893897849No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCTA150.00825001550.26877649
CGATACA150.00827577550.22914546
TACCGTG204.087093E-450.2291446
AGTGCCG150.00828867850.20935443
CGTAAAC150.00828867850.20935443
TACGTAG252.0386768E-550.18957540
ATACGGT150.00831021650.17640739
AATACGG252.0450194E-550.1632338
CGACCTA150.00832315850.15665437
ATCGCGT150.00832315850.15665437
ATCGCGC150.00832315850.15665437
CATCGCG301.0246258E-650.15007436
TCATCCG252.0481968E-550.1500736
ACTCGAT600.050.1237735
ACGCGTT252.0545638E-550.12376835
CGAATTG150.00835341550.1106334
AACTCGA600.050.1106334
CGATCTA204.135017E-450.1106334
CGACCAA204.1510904E-450.0712531
ACGTGTA150.00838375550.06469730
Produced by FastQC (version 0.11.5)