##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212817_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 383668 Sequences flagged as poor quality 0 Sequence length 56 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.343390639824015 39.0 38.0 40.0 35.0 40.0 2 38.29436387710208 39.0 38.0 40.0 35.0 40.0 3 38.33668432081904 39.0 38.0 40.0 35.0 40.0 4 38.43947371164653 39.0 38.0 40.0 36.0 40.0 5 38.15431310403787 39.0 38.0 40.0 35.0 40.0 6 38.19346674729193 39.0 38.0 40.0 35.0 40.0 7 38.58907180166185 39.0 38.0 40.0 37.0 40.0 8 38.56175391223662 39.0 38.0 40.0 36.0 40.0 9 38.55598851090005 39.0 38.0 40.0 36.0 40.0 10 37.713666503331005 39.0 38.0 40.0 33.0 40.0 11 37.88768675000261 39.0 38.0 40.0 35.0 40.0 12 37.84298143186297 39.0 38.0 40.0 34.0 40.0 13 37.830718225132145 39.0 38.0 40.0 34.0 40.0 14 37.82554187474587 39.0 38.0 40.0 34.0 40.0 15 37.78703983652533 39.0 38.0 40.0 33.0 40.0 16 37.69564832094415 39.0 38.0 40.0 33.0 40.0 17 37.48926415546775 39.0 38.0 40.0 33.0 40.0 18 37.41840341128267 39.0 37.0 40.0 33.0 40.0 19 37.488854947506695 39.0 38.0 40.0 33.0 40.0 20 37.34967471876727 39.0 37.0 40.0 33.0 40.0 21 37.18010363126453 39.0 37.0 40.0 33.0 40.0 22 37.10290146689325 39.0 37.0 40.0 33.0 40.0 23 36.90000469155624 39.0 36.0 40.0 32.0 40.0 24 36.745608182074086 39.0 36.0 40.0 31.0 40.0 25 36.73761689794301 39.0 36.0 40.0 32.0 40.0 26 36.306444634423514 38.0 36.0 40.0 31.0 40.0 27 36.234272860911 38.0 36.0 40.0 31.0 40.0 28 35.9617768487338 38.0 35.0 40.0 30.0 40.0 29 35.88065202206074 38.0 35.0 40.0 30.0 40.0 30 35.62833491456155 38.0 35.0 40.0 29.0 40.0 31 35.196573600091746 38.0 35.0 40.0 28.0 40.0 32 35.0206376346216 38.0 35.0 39.0 28.0 40.0 33 34.857504404850026 38.0 34.0 39.0 27.0 40.0 34 35.14074408081988 38.0 35.0 40.0 28.0 40.0 35 34.9967185170512 38.0 35.0 40.0 28.0 40.0 36 34.98230501370977 38.0 35.0 40.0 28.0 40.0 37 34.77736480498765 38.0 35.0 40.0 27.0 40.0 38 34.59104225528321 38.0 34.0 40.0 26.0 40.0 39 34.48320422865603 38.0 34.0 40.0 26.0 40.0 40 34.23990012198046 38.0 34.0 40.0 25.0 40.0 41 33.73646225382362 38.0 33.0 39.0 23.0 40.0 42 33.51047259609871 38.0 33.0 39.0 22.0 40.0 43 33.423762211078326 38.0 33.0 39.0 22.0 40.0 44 33.209053139693694 38.0 33.0 39.0 20.0 40.0 45 32.866293253542125 37.0 32.0 39.0 17.0 40.0 46 32.2320626166373 36.0 32.0 39.0 8.0 40.0 47 32.05458886328805 36.0 32.0 39.0 2.0 40.0 48 31.867200287748783 36.0 31.0 39.0 2.0 40.0 49 31.584020559442017 36.0 31.0 39.0 2.0 40.0 50 31.207609182939418 36.0 31.0 39.0 2.0 40.0 51 30.61481280690597 36.0 30.0 39.0 2.0 40.0 52 30.41755893115923 36.0 30.0 39.0 2.0 40.0 53 30.09675292179697 35.0 30.0 39.0 2.0 40.0 54 29.704689992389252 35.0 29.0 38.0 2.0 40.0 55 29.306825171763087 35.0 29.0 38.0 2.0 40.0 56 17.0553499379672 20.0 2.0 24.0 2.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.5009697059997436 1 2 -0.5689693783760035 1 3 -0.4115981341462458 1 4 -0.3475654177320493 1 5 -0.5605143132233579 1 6 -0.40174463003928906 1 7 -0.23030107139880585 1 8 -0.35561523000812656 1 9 -0.48315199920289587 1 10 -0.2607819004813692 1 11 -0.5806267069627893 1 12 -0.7576899621421447 1 13 -0.5653612502012066 1 14 -0.5530013885383624 1 15 -0.5096952456616322 1 16 -0.40719291883207376 1 17 -0.7138758346017653 1 18 -0.5741079938009293 1 19 -0.43427637453535795 1 20 -0.526741578721662 1 21 -0.5166822552721797 1 22 -0.37325216492020985 1 23 -0.2462453840157366 1 24 -0.28092321234701956 1 25 0.220926127610106 1 26 -0.3986762897320375 1 27 0.07788087538265387 1 28 -0.06815144291706332 1 29 -0.2020023454533657 1 30 -0.22853190100566678 1 31 0.05200438709592703 1 32 0.21222926438894518 1 33 -0.3765286104936152 1 34 -0.33634064076123593 1 35 -0.3146879442594752 1 36 -0.12272902243411465 1 37 -0.05367339887999378 1 38 -0.4329753203027309 1 39 -0.07064900435211996 1 40 -0.39772735268534376 1 41 0.2716700950384592 1 42 0.05980722236932223 1 43 0.27321180972634806 1 44 0.052603170849458536 1 45 0.07917339468977502 1 46 0.4840458333549549 1 47 -0.06751893958511701 1 48 -0.1115219080440717 1 49 0.3707561835266304 1 50 0.2849493577709836 1 51 0.5475593726148027 1 52 0.9313525752147171 1 53 0.6098014553818096 1 54 0.569106011913405 1 55 0.12283156234613557 1 56 0.6093952248040431 2 1 -0.4871670044033465 2 2 -0.5124978270047436 2 3 -0.31160632080199946 2 4 -0.42758179104355065 2 5 -0.673498349244646 2 6 -0.5685968609029786 2 7 -0.3954914111450165 2 8 -0.4009119962791061 2 9 -0.2766927278152522 2 10 -0.8240320028145689 2 11 -0.9836967028694588 2 12 -0.8835516076599532 2 13 -0.7680300999760732 2 14 -0.4461982776091773 2 15 -0.5032032276509923 2 16 -0.1829276711857375 2 17 -0.525410832555103 2 18 -0.07318290170105257 2 19 -0.07313842938594917 2 20 -0.1949282344727905 2 21 -0.12554840345064378 2 22 -0.24838110474829023 2 23 0.015948639725564817 2 24 -0.42617085868349136 2 25 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19 1849.0 20 2259.0 21 2438.0 22 2445.0 23 2849.0 24 3321.0 25 3717.0 26 4109.0 27 4809.0 28 5807.0 29 6607.0 30 8170.0 31 9763.0 32 11674.0 33 14884.0 34 19912.0 35 27972.0 36 40393.0 37 61123.0 38 76827.0 39 61656.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 40.36901696258223 45.53311717422355 3.7811336885015168 10.316732174692703 11 27.20268565530615 21.706266876570368 28.48269858315001 22.608348884973466 12 27.75237966158241 14.890999509993014 30.340554854718143 27.016065973706432 13 21.124253260631587 16.721488370153363 35.10978241604721 27.044475953167847 14 21.408092413232275 21.1844615657287 34.99379672008091 22.41364930095812 15 24.176371237632534 23.78853592168229 31.229865404464274 20.8052274362209 16 27.144562486316293 24.448742141643294 26.39678054985039 22.00991482219002 17 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24.2708257187314 30 23.913974898895425 23.17123509556603 28.004138233726607 24.91065177181194 31 25.909375979931013 23.55963206856904 27.193216264241666 23.337775687258283 32 25.506769118185147 22.718885578380636 29.319951910511733 22.45439339292248 33 26.241042038689503 22.529223673855693 28.55148360310708 22.67825068434772 34 23.303661294328325 24.587009659751903 31.365305935714936 20.74402311020483 35 23.260320905964353 27.142315387532527 29.484902773600453 20.112460932902668 36 24.617462452951678 26.290000889926556 28.172980782821277 20.919555874300492 37 25.508057764755126 26.271714106320637 27.920939723733778 20.299288405190456 38 23.83686528321054 27.279010949306436 27.937749710689058 20.946374056793964 39 24.092829324889543 25.739421034573972 28.68636710064193 21.481382539894557 40 23.362617086965408 26.528617181316406 27.585872510837262 22.522893220880924 41 22.39639719933627 26.007061950660713 29.614056794440614 21.982484055562402 42 22.344746924571695 25.5543113689229 29.73495328181177 22.365988424693636 43 23.161273909405875 24.808082185673108 28.65876527408775 23.371878630833265 44 22.45689033425563 24.221665639465506 29.48294627519375 23.838497751085118 45 23.68860823917372 25.03548201763476 28.709346527484463 22.566563215707056 46 22.93483393672281 26.521066162967628 28.72291305944698 21.821186840862588 47 21.51206285124802 25.022377501227155 29.95566498583852 23.509894661686307 48 23.060520835795185 24.591726604536106 28.750745120282765 23.59700743938594 49 23.14982245539077 26.294186037356543 28.357535213670275 22.19845629358241 50 21.04012331900389 26.093944081029196 29.746508018623903 23.11942458134301 51 22.336705710640576 25.82677496075477 29.97469404470019 21.86182528390447 52 22.957042064838795 26.680465881092097 28.01879705173129 22.34369500233781 53 21.89218228292365 25.783308508095132 29.86568101276464 22.458828196216583 54 22.218369062631357 26.487757461118118 29.12253047498949 22.171343001261036 55 22.01626512041131 26.228689485498595 27.755581606850903 23.999463787239186 56 21.192928419564318 27.033263339369107 27.66741391136609 24.106394329700482 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 316.0 7 632.0 8 1456.5 9 2281.0 10 2788.5 11 3296.0 12 2646.5 13 1907.5 14 1818.0 15 2030.5 16 2243.0 17 1992.0 18 1741.0 19 2037.5 20 2334.0 21 2855.0 22 3912.0 23 4448.0 24 5058.5 25 5669.0 26 7285.0 27 8901.0 28 8886.5 29 8549.5 30 8227.0 31 9504.5 32 10782.0 33 10382.0 34 9982.0 35 10405.5 36 10829.0 37 11047.5 38 11951.5 39 12637.0 40 13838.5 41 15040.0 42 15348.5 43 15657.0 44 15966.0 45 16275.0 46 16806.5 47 18479.5 48 19621.0 49 19572.5 50 19524.0 51 20252.5 52 20981.0 53 21496.0 54 24493.0 55 26975.0 56 26723.5 57 26472.0 58 23568.5 59 20665.0 60 18311.5 61 15958.0 62 13861.5 63 10779.0 64 9793.0 65 7425.5 66 5058.0 67 4200.0 68 3342.0 69 3064.5 70 2787.0 71 2072.0 72 1047.0 73 737.0 74 589.0 75 441.0 76 341.0 77 241.0 78 200.0 79 122.5 80 86.0 81 58.0 82 30.0 83 23.0 84 16.0 85 10.0 86 4.0 87 3.5 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0010425680536297007 23 0.004170272214518803 24 2.606420134074252E-4 25 0.028149337448001917 26 0.08966085261215426 27 0.10217166925571067 28 0.12458688240874922 29 0.1647257524734927 30 0.23353524401305295 31 0.25751430924653607 32 0.27315283005098157 33 0.30938206991461364 34 0.3005202414587612 35 0.34222296360394927 36 0.4206762096395842 37 0.3721967951458031 38 0.44986811514121583 39 0.48192708279032914 40 0.5509972163432968 41 0.5700240833220388 42 0.6096416693599674 43 0.6216312019767091 44 0.6185034978158199 45 0.6487379713710812 46 0.6484773293576739 47 0.7050366462670851 48 0.7446542323050137 49 0.6873129893553802 50 0.7472606524390879 51 0.7115526966022707 52 0.7741067798200527 53 0.7837505343161275 54 0.7892240165976835 55 0.839788567198724 56 0.823368120354056 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 383668.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.100733169580376 #Duplication Level Percentage of deduplicated Percentage of total 1 60.03299999989233 15.669053143666083 2 11.583999999979223 6.047017860717536 3 6.1989999999888825 4.853953347538157 4 4.30999999999227 4.499766398427586 5 3.202000000172372 4.178727380674769 6 2.459999999996804 3.8524682158250583 7 2.032999999996363 3.714395337356338 8 1.631999999997073 3.4077117226143017 9 1.2989999999976702 3.051436714850169 >10 6.642999999988087 29.504529782172433 >50 0.36699999999934174 6.611054704511156 >100 0.21899999999960723 10.377651508206545 >500 0.013999999999974892 2.4769595777887354 >1k 0.0049999999999910325 1.7552743056511322 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACTTATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1801 0.4694162661467727 No Hit ACTTATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1385 0.36098918856928386 No Hit ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 1287 0.3354462712553562 No Hit ACTTATGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 1172 0.3054724397135023 No Hit ACTTATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1080 0.2814933744800192 No Hit ACTTATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 974 0.25386532105883214 No Hit ACTTATGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT 858 0.22363084750357082 No Hit ACTTATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 829 0.2160722291147555 No Hit ACTTATGGGACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTC 806 0.21007746280638467 No Hit ACTTATGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTC 737 0.19209316388127234 No Hit ACTTATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA 703 0.1832313354254199 No Hit ACTTATGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 638 0.16628960455393724 No Hit ACTTATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 632 0.1647257524734927 No Hit ACTTATGGGCTGTGCTGTAAGACAGCTTGACACAACTCGATGTCATTCTTTTTCCC 591 0.15403942992378827 No Hit ACTTATGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC 578 0.15065108374949177 No Hit ACTTATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 556 0.1449169594545284 No Hit ACTTATGGGGGGCTGGAGAGATGGCTCAGCGAGTAAGAGCACCTCACTGCTCTTCC 554 0.14439567542771356 No Hit ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 533 0.1389221931461576 No Hit ACTTATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC 501 0.13058164871712 No Hit ACTTATGGGGAGGGTCCAGGCCATCCTCAGCTGCCGAGCCTCATTGTGACCTTTGT 490 0.12771458656963836 No Hit ACTTATGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT 464 0.12093789422104528 No Hit ACTTATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 461 0.120155968180823 No Hit ACTTATGGGAGTCTTAAGCATATAAAAGAAACATTTGTAAAGATGTATAAGAGTAA 453 0.11807083207356361 No Hit ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 451 0.11754954804674876 No Hit ACTTATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 446 0.11624633797971164 No Hit ACTTATGGGGTGTGTTCAAACACCCCTCCCACAGGACTCACTTTGTCCCAACAATT 445 0.1159856959663042 No Hit ACTTATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG 444 0.11572505395289678 No Hit ACTTATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 415 0.10816643556408145 No Hit ACTTATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 412 0.10738450952385917 No Hit ACTTATGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCA 410 0.10686322549704431 No Hit ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC 402 0.1047780893897849 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0013032100670371258 0.0 0.0 0.0 0.0 11 0.003127704160889102 0.0 0.0 0.0 0.0 12 0.003127704160889102 0.0 0.0 0.0 0.0 13 0.003127704160889102 0.0 0.0 0.0 0.0 14 0.004691556241333653 0.0 0.0 0.0 0.0 15 0.004691556241333653 0.0 0.0 0.0 0.0 16 0.005212840268148503 0.0 0.0 0.0 0.0 17 0.005212840268148503 0.0 0.0 0.0 0.0 18 0.005734124294963353 0.0 0.0 0.0 0.0 19 0.006255408321778204 0.0 0.0 0.0 0.0 20 0.007037334362000479 0.0 0.0 0.0 0.0 21 0.00755861838881533 0.0 0.0 0.0 0.0 22 0.007819260402222755 0.0 0.0 0.0 0.0 23 0.008861828455852455 0.0 0.0 0.0 0.0 24 0.011728890603334133 0.0 0.0 0.0 0.0 25 0.013032100670371258 0.0 0.0 0.0 0.0 26 0.014074668724000958 0.0 0.0 0.0 0.0 27 0.01563852080444551 0.0 0.0 0.0 0.0 28 0.020851361072594013 0.0 0.0 0.0 0.0 29 0.02267585516644599 0.0 0.0 0.0 0.0 30 0.02919190550163162 0.0 0.0 0.0 0.0 31 0.029713189528446468 0.0 0.0 0.0 0.0 32 0.03284089368933557 0.0 0.0 0.0 0.0 33 0.03362281972955785 0.0 0.0 0.0 0.0 34 0.034144103756372696 0.0 0.0 0.0 0.0 35 0.04066015409155833 0.0 0.0 0.0 0.0 36 0.042484648185410305 0.0 0.0 0.0 0.0 37 0.044048500265854856 0.0 0.0 0.0 0.0 38 0.044309142279262276 0.0 0.0 0.0 0.0 39 0.04587299435970683 0.0 0.0 0.0 0.0 40 0.04743684644015138 0.0 0.0 0.0 0.0 41 0.04900069852059593 0.0 0.0 0.0 0.0 42 0.05056455060104048 0.0 0.0 0.0 0.0 43 0.05134647664126276 0.0 0.0 0.0 0.0 44 0.05395289677533701 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGCTA 15 0.008250015 50.268776 49 CGATACA 15 0.008275775 50.229145 46 TACCGTG 20 4.087093E-4 50.22914 46 AGTGCCG 15 0.008288678 50.209354 43 CGTAAAC 15 0.008288678 50.209354 43 TACGTAG 25 2.0386768E-5 50.189575 40 ATACGGT 15 0.008310216 50.176407 39 AATACGG 25 2.0450194E-5 50.16323 38 CGACCTA 15 0.008323158 50.156654 37 ATCGCGT 15 0.008323158 50.156654 37 ATCGCGC 15 0.008323158 50.156654 37 CATCGCG 30 1.0246258E-6 50.150074 36 TCATCCG 25 2.0481968E-5 50.15007 36 ACTCGAT 60 0.0 50.12377 35 ACGCGTT 25 2.0545638E-5 50.123768 35 CGAATTG 15 0.008353415 50.11063 34 AACTCGA 60 0.0 50.11063 34 CGATCTA 20 4.135017E-4 50.11063 34 CGACCAA 20 4.1510904E-4 50.07125 31 ACGTGTA 15 0.008383755 50.064697 30 >>END_MODULE