FastQCFastQC Report
Tue 31 May 2016
SRR212816_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212816_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311020
Sequences flagged as poor quality0
Sequence length58
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA39251.261976721754228No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG13590.4369493923220372No Hit
GAACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11880.3819690052086682No Hit
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA10580.34017105009324156No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA9950.31991511799884254No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA9850.3166998906822712No Hit
GAACGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA8370.2691145263970163No Hit
GAACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8260.2655777763487879No Hit
GAACGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC8160.26236254903221656No Hit
GAACGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC8150.2620410263005594No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA8070.25946884444730245No Hit
GAACGCGGGAGCCCCAAATCCTGCAGCAGAGCCCCAAAACTGGCCTGTAAAAGCAGCT7620.24500032152273166No Hit
GAACGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT6690.21509870747861873No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA6550.21059738923541893No Hit
GAACGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCT6430.20673911645553342No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA6280.2019162754806765No Hit
GAACGCGGGGATTTTATGTAGTATCTAAATTACCATGTATGGTTCTGTAAGTGGAAAC6180.19870104816410522No Hit
GAACGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC5840.18776927528776285No Hit
GAACGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT5400.1736222750948492No Hit
GAACGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA5320.17105009324159218No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG5180.16654877499839238No Hit
GAACGCGGGATAATCAAGTGACATGATTCTCCCATGTCCCTGGCCAGCCCGGAGGGTC5030.16172593402353547No Hit
GAACGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC4930.15851070670696418No Hit
GAACGCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG4760.153044820268793No Hit
GAACGCGGGGTGAAAGAGCCCTCCTCCTCACAGCACAATCCTTACCTGCCAACGCCAG4580.14725741109896467No Hit
GAACGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG4200.13503954729599382No Hit
GAACGCGGGACTGTTGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTG4160.1337534563693653No Hit
GAACGCGGGATATTTATTATTTATAACAATAACTTGTATTTATACTACACTTAATATA4050.1302167063211369No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCAACTGCTCTTCCGA3960.12732300173622274No Hit
GAACGCGGGAGTCTCTCTCATTGTTCTAGTTTTAACTAAACTGCATTCTAGCAAGTTG3850.12378625168799433No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGA3810.12250016076136583No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGTGCTTAAGATCACTGACTGCTCTTCCAGA3780.12153559256639443No Hit
GAACGCGGGATTTCTGTCTGTGTGCCACATGTATGTCTGTTGCCCACAGAGTCTAGAA3720.11960645617645167No Hit
GAACGCGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTT3610.11606970612822326No Hit
GAACGCGGGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG3570.11478361520159475No Hit
GAACGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAA3510.11285447881165198No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTTGGTAAGAGCACCCGACTGCTCTTCCAA3430.11028229695839496No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCGA3410.1096392514950807No Hit
GAACGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3330.10706706964182368No Hit
GAACGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3280.10545945598353804No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA3250.10449488778856665No Hit
GAACGCGGGAGCTTTCAGAGGTCTGGTCTGTTCTTTTCTCTCTGTAGGCCCTAAGAAG3210.10320879686193814No Hit
GAACGCGGGGTTTTCTATCAATGCTGACTTTTGCACATTTTCAAACATTTAATTTGTA3170.10192270593530961No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGAT203.430617E-452.04025349
GCCGATC150.007193160752.04025351
TCGTCAA150.007193160752.04025348
GTAACTT150.007193160752.04025349
GCCAACG459.094947E-1152.04025348
TTAACAC150.007197742452.03187646
CTCGACA150.007197742452.03187647
GTCACGG150.00720232652.02350242
CGGTCAC150.00720232652.02350240
ATACCCC401.880835E-952.023536
GGTACTT150.007206911752.0151325
CTTATAG150.007206911752.0151325
TATAGTC203.4388067E-452.0151326
TCGGTAG150.007206911752.0151326
TCTACTA503.6379788E-1252.01512527
AACGTTA150.007211499452.0067633
GATACCC401.886292E-952.0067635
ATACTCG150.007211499452.0067632
TACTCGG150.007211499452.0067633
ATGCGTC150.007211499452.0067630
Produced by FastQC (version 0.11.5)