FastQCFastQC Report
Tue 31 May 2016
SRR212815_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212815_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences313167
Sequences flagged as poor quality0
Sequence length58
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA39341.2561987693467063No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG13530.43203785839504166No Hit
GAACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11660.37232530886076753No Hit
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA10140.32378890496125073No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA9740.31101616709295676No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA9500.30335252437198046No Hit
GAACGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA7860.2509842991119754No Hit
GAACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7770.24811043309160927No Hit
GAACGCGGGAGCCCCAAATCCTGCAGCAGAGCCCCAAAACTGGCCTGTAAAAGCAGCT7670.24491724862453582No Hit
GAACGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7540.24076610881734029No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA7490.23916951658380353No Hit
GAACGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7350.23469905832990065No Hit
GAACGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT6960.22224563890831409No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA6360.20308653210587324No Hit
GAACGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCT6310.2014899398723365No Hit
GAACGCGGGGATTTTATGTAGTATCTAAATTACCATGTATGGTTCTGTAAGTGGAAAC6230.1989353922986777No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA6040.1928683418112381No Hit
GAACGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT5680.18137287772977356No Hit
GAACGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC5450.17402855345550458No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG5180.1654069553944062No Hit
GAACGCGGGATAATCAAGTGACATGATTCTCCCATGTCCCTGGCCAGCCCGGAGGGTC5130.16381036316086944No Hit
GAACGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA5120.1634910447141621No Hit
GAACGCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG4770.15231489907940493No Hit
GAACGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC4540.14497057480513592No Hit
GAACGCGGGGTGAAAGAGCCCTCCTCCTCACAGCACAATCCTTACCTGCCAACGCCAG4230.13507170295720813No Hit
GAACGCGGGATATTTATTATTTATAACAATAACTTGTATTTATACTACACTTAATATA4070.12996260780989058No Hit
GAACGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3950.1261307864494024No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGA3930.1254921495559877No Hit
GAACGCGGGACTGTTGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTG3810.12166032819549953No Hit
GAACGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3690.11782850683501137No Hit
GAACGCGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTT3570.11399668547452317No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCAACTGCTCTTCCGA3460.11048418256074234No Hit
GAACGCGGGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG3410.10888759032720562No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGTGCTTAAGATCACTGACTGCTCTTCCAGA3390.10824895343379093No Hit
GAACGCGGGAGTCTCTCTCATTGTTCTAGTTTTAACTAAACTGCATTCTAGCAAGTTG3370.10761031654037623No Hit
GAACGCGGGAGCTTTCAGAGGTCTGGTCTGTTCTTTTCTCTCTGTAGGCCCTAAGAAG3350.10697167964696151No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTTGGTAAGAGCACCCGACTGCTCTTCCAA3320.10601372430683949No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCGA3300.10537508741342479No Hit
GAACGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAA3270.10441713207330273No Hit
GAACGCGGGATTTCTGTCTGTGTGCCACATGTATGTCTGTTGCCCACAGAGTCTAGAA3250.10377849517988805No Hit
GAACGCGGGGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGGAT3230.10313985828647335No Hit
GAACGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3190.10186258449964396No Hit
GAACGCGGGGTTTTCTATCAATGCTGACTTTTGCACATTTTCAAACATTTAATTTGTA3180.10154326605293662No Hit
GAACGCGGGACAGCAACAGCCACCACTGCCCCCATCTTCCCAGTCAGAGGGAATGGGA3180.10154326605293662No Hit
GAACGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3160.1009046291595219No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGC150.007138872552.14010625
CTCGTAA307.829549E-752.14010627
GTAAGAC150.007138872552.14010624
TTCGAAC150.007138872552.14010627
ACGAACG150.007138872552.14010624
TACGAAC150.00714792952.12339823
TATCCGA203.403713E-452.12339823
ACGTTCA150.00715246152.11504422
AGATACA150.007156994752.10669321
TGCCCGA150.00719789452.03167746
CGAGTTA203.4334432E-452.03167750
CGTCTAT150.00719789452.03167744
TATCTCG150.00719789452.03167750
AACGATC203.4334432E-452.03167745
ACGTCTT150.00719789452.03167742
ACGTCTA150.00719789452.03167743
CGGTCAG150.00719789452.03167748
GATTATC150.00719789452.03167743
TATGTCG150.00719789452.03167752
ATACTAC503.6379788E-1252.03167342
Produced by FastQC (version 0.11.5)