FastQCFastQC Report
Tue 31 May 2016
SRR212814_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212814_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314468
Sequences flagged as poor quality0
Sequence length58
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA36671.1660963913657352No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG11750.3736469211493697No Hit
GAACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10480.33326125392726763No Hit
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA10200.3243573272956231No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA8950.28460765483292416No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA8540.27156976226515894No Hit
GAACGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7870.2502639378251523No Hit
GAACGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA7820.24867395092664438No Hit
GAACGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7620.24231400333261255No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA6970.2216441736520091No Hit
GAACGCGGGAGCCCCAAATCCTGCAGCAGAGCCCCAAAACTGGCCTGTAAAAGCAGCT6930.22037218413320275No Hit
GAACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6900.21941819199409796No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA6390.20320032562931684No Hit
GAACGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT6360.20224633349021204No Hit
GAACGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCT6340.20161033873080883No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA6080.1933424068585675No Hit
GAACGCGGGGATTTTATGTAGTATCTAAATTACCATGTATGGTTCTGTAAGTGGAAAC5330.16949260338094815No Hit
GAACGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT5060.1609066741290052No Hit
GAACGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC4990.15868069247109404No Hit
GAACGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA4880.15518272129437652No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG4620.14691478942213515No Hit
GAACGCGGGATAATCAAGTGACATGATTCTCCCATGTCCCTGGCCAGCCCGGAGGGTC4500.14309882086571607No Hit
GAACGCGGGGTGAAAGAGCCCTCCTCCTCACAGCACAATCCTTACCTGCCAACGCCAG4180.13292290471526516No Hit
GAACGCGGGACTGTTGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTG4020.1278349466400397No Hit
GAACGCGGGATATTTATTATTTATAACAATAACTTGTATTTATACTACACTTAATATA3990.12688095450093492No Hit
GAACGCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3890.12370098070391901No Hit
GAACGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3780.12020300952720149No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGTGCTTAAGATCACTGACTGCTCTTCCAGA3670.11670503835048399No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCGA3650.1160690435910808No Hit
GAACGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC3640.11575104621137922No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGA3630.11543304883167763No Hit
GAACGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3550.11288906979406489No Hit
GAACGCGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTT3390.10780111171883944No Hit
GAACGCGGGATTTCTGTCTGTGTGCCACATGTATGTCTGTTGCCCACAGAGTCTAGAA3350.10652912220003308No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCAACTGCTCTTCCGA3310.10525713268122672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTAA150.00704485552.3152059
CGGGATC11600.052.089716
AGTCCCG150.007207134352.0148351
CCGGATC150.007207134352.0148350
TTACGAA150.007207134352.0148350
CGACGGC203.4416592E-452.00654244
TAGTCTG203.4416592E-452.00654237
CCCATAG353.8675353E-852.0065437
TAGTACC150.007211681552.0065443
CTCGTAA203.44437E-451.9982527
TCTCGTA203.44437E-451.9982526
CGAATCG203.44437E-451.9982526
AATCGGG203.44437E-451.9982534
ATAGGGC307.978251E-751.99824540
TCTAGAC150.007216230551.99824534
GACGCTA150.007216230551.99824540
TACGTCA150.007216230551.99824535
CTACTAG251.6558697E-551.98167428
CCGGAAT150.007225335651.9816728
GTTACAA203.4497963E-451.9816729
Produced by FastQC (version 0.11.5)