FastQCFastQC Report
Tue 31 May 2016
SRR212813_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212813_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328853
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC45221.3750824836629132No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC24810.7544404338716691No Hit
GAACGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT14850.45156954627143436No Hit
GAACGCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC12150.3694659924039008No Hit
GAACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11330.34453083900709436No Hit
GAACGCGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTC10250.31168941746008094No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT10010.30439132378296685No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC9830.2989177535251313No Hit
GAACGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG9370.28492974064399595No Hit
GAACGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC8160.2481351850218791No Hit
GAACGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT7850.23870848068894007No Hit
GAACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7140.2171182868941442No Hit
GAACGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA6810.20708340808811232No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC6290.19127087178769847No Hit
GAACGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC6020.18306051640094512No Hit
GAACGCGGGGATTTTATGTAGTATCTAAATTACCATGTATGGTTCTGTAAGTGGAA5900.17941146956238807No Hit
GAACGCGGGAGCCCCAAATCCTGCAGCAGAGCCCCAAAACTGGCCTGTAAAAGCAG5690.1730256375949132No Hit
GAACGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC5680.1727215503583668No Hit
GAACGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA5440.16542345668125272No Hit
GAACGCGGGATAATCAAGTGACATGATTCTCCCATGTCCCTGGCCAGCCCGGAGGG4860.14778639696156035No Hit
GAACGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA4520.13744743091898204No Hit
GAACGCGGGATATTTATTATTTATAACAATAACTTGTATTTATACTACACTTAATA4330.13166977342460004No Hit
GAACGCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG4100.12467576698403239No Hit
GAACGCGGGGTGAAAGAGCCCTCCTCCTCACAGCACAATCCTTACCTGCCAACGCC3920.11920219672619681No Hit
GAACGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA3880.11798584778001113No Hit
GAACGCGGGATTAGCATGTTTTCGAAATGCTATAAGAGTAAATCCCAGACATTACA3870.1176817605434647No Hit
GAACGCGGGGCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC3860.11737767330691828No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCAACTGCTCTTCC3800.11555314988763976No Hit
GAACGCGGGACTGTTGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTT3740.11372862646836124No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTTGGTAAGAGCACCCGACTGCTCTTCC3710.11281636475872199No Hit
GAACGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3670.11160001581253631No Hit
GAACGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA3650.11099184133944347No Hit
GAACGCGGGAGTCTCTCTCATTGTTCTAGTTTTAACTAAACTGCATTCTAGCAAGT3560.10825505621052567No Hit
GAACGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG3490.10612644555470074No Hit
GAACGCGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC3490.10612644555470074No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCC3490.10612644555470074No Hit
GAACGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT3490.10612644555470074No Hit
GAACGCGGGATTTCTGTCTGTGTGCCACATGTATGTCTGTTGCCCACAGAGTCTAG3480.1058223583181543No Hit
GAACGCGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTC3440.10460600937196862No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC3440.10460600937196862No Hit
GAACGCGGGGTTTTCTATCAATGCTGACTTTTGCACATTTTCAAACATTTAATTTG3390.10308557318923653No Hit
GAACGCGGGATCAGGAAAGAGGCAAGATTGCATCCTTTCCTGGCCTCCCCATGGGC3370.10247739871614368No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGTGCTTAAGATCACTGACTGCTCTTCCA3350.10186922424305085No Hit
GAACGCGGGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3330.101261049769958No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCA3330.101261049769958No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTAC252.0054793E-550.3263249
CTCACCG204.0474316E-450.32631749
CAAGCGT252.016443E-550.27997247
CTATTAG204.0689597E-450.2722546
AACGATC252.0237798E-550.24912345
CGTCTAT150.00826172450.2491244
GATAAAT150.00826673650.24141343
ACGTCTA150.00826673650.24141343
ATACTAC355.0835297E-850.2337142
CGGAATC204.0967722E-450.20291541
ATAGGGC204.0998717E-450.1952240
ATATGCG150.0082968550.1952239
TGCGGAA150.0082968550.1952239
CGCAGGT204.0998717E-450.1952239
ACCGTTG204.0998717E-450.1952240
GCGGAAT204.0998717E-450.1952240
TCATACG150.0082968550.1952239
GTAAATC301.0189087E-650.18752738
AGGGTAC150.00834722350.11842333
TATTAGA150.00834722350.11842333
Produced by FastQC (version 0.11.5)