FastQCFastQC Report
Tue 31 May 2016
SRR212812_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212812_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297187
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC41751.404839377227133No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC22870.7695491390942403No Hit
GAACGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT15480.5208841571131981No Hit
GAACGCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC13780.46368111660335076No Hit
GAACGCGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTC10840.36475350536867357No Hit
GAACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9750.3280762617476538No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC9640.32437488853819313No Hit
GAACGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG8340.28063138697183926No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT7810.2627974978717104No Hit
GAACGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC7480.25169337824332827No Hit
GAACGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA7060.2375608623526601No Hit
GAACGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT6530.21972697325253124No Hit
GAACGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC6440.2166985769902452No Hit
GAACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6160.20727689972979976No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC6070.2042485034675137No Hit
GAACGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA5690.1914619414711949No Hit
GAACGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC5550.18675110284097218No Hit
GAACGCGGGAGCCCCAAATCCTGCAGCAGAGCCCCAAAACTGGCCTGTAAAAGCAG5230.1759834716861774No Hit
GAACGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA4590.15444820937658782No Hit
GAACGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA4470.15041034769353975No Hit
GAACGCGGGGATTTTATGTAGTATCTAAATTACCATGTATGGTTCTGTAAGTGGAA4420.14872790532560307No Hit
GAACGCGGGGCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC4030.13560485485569693No Hit
GAACGCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG3960.13324943554058555No Hit
GAACGCGGGATAATCAAGTGACATGATTCTCCCATGTCCCTGGCCAGCCCGGAGGG3920.13190348164623622No Hit
GAACGCGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTC3800.12786561996318815No Hit
GAACGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG3690.12416424675372746No Hit
GAACGCGGGATTAGCATGTTTTCGAAATGCTATAAGAGTAAATCCCAGACATTACA3620.1218088274386161No Hit
GAACGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA3560.11978989659709206No Hit
GAACGCGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC3500.11777096575556803No Hit
GAACGCGGGATATTTATTATTTATAACAATAACTTGTATTTATACTACACTTAATA3460.11642501186121869No Hit
GAACGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT3370.11339661559893266No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTTGGTAAGAGCACCCGACTGCTCTTCC3360.11306012712534533No Hit
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC3270.11003173086305928No Hit
GAACGCGGGATTTCTGTCTGTGTGCCACATGTATGTCTGTTGCCCACAGAGTCTAG3250.10935875391588461No Hit
GAACGCGGGATCAGGAAAGAGGCAAGATTGCATCCTTTCCTGGCCTCCCCATGGGC3220.1083492884951226No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC3210.10801280002153525No Hit
GAACGCGGGGTTTTCTATCAATGCTGACTTTTGCACATTTTCAAACATTTAATTTG3070.10330196139131255No Hit
GAACGCGGGGTGAAAGAGCCCTCCTCCTCACAGCACAATCCTTACCTGCCAACGCC3060.1029654729177252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGTC402.5302143E-950.30680549
ACTTGCC150.008223548550.306849
CTCCCGT150.008223548550.306850
GCGCCAA150.00824014850.28114747
TAGATTG150.00824014850.28114747
ACACGAT204.0749996E-450.25552445
GGCGACT150.00825677350.2555244
GCATCGG150.00827342350.2299241
TATACAC204.0921126E-450.21286839
CGGACAA204.0955422E-450.20434636
GATAGTA204.0955422E-450.20434637
CGACCTA150.00829009750.2043436
CCGTAGC150.00829009750.2043437
CGCCGAT150.00829009750.2043438
GCCGTAG150.00829009750.2043436
ACTAAAC301.0174081E-650.19582435
CGCCGTA150.00829566150.19582435
GCGCCGT150.00830679750.17879534
TATGATA204.1058444E-450.17879534
CGCAGTA150.00830679750.17879534
Produced by FastQC (version 0.11.5)