FastQCFastQC Report
Tue 31 May 2016
SRR212811_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212811_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326084
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21320.6538192612946357No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA19780.6065921664356423No Hit
GCGTCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG16550.5075379350106107No Hit
GCGTCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13730.42105715091816837No Hit
GCGTCCGGGATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGCTT10940.3354963751671348No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA10510.32230958894027306No Hit
GCGTCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA8560.2625090467486905No Hit
GCGTCCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT8520.26128236896014523No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8210.2517756160989193No Hit
GCGTCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA7670.23521546595355797No Hit
GCGTCCGGGAGTTCAAGTTTAGAGCTAAATTCTTTCCTGAAGATGTTTCTGAGGAATT6460.1981084628500632No Hit
GCGTCCGGGAGCAGTGAGTTTTTTGTAACCAGTCTCTTGTCTCAGGCTCTCTGCCTTG6250.19166840446020045No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA6050.18553501551747403No Hit
GCGTCCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5920.18154831270470187No Hit
GCGTCCGGGAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCA5780.17725494044479337No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA5680.17418824597343016No Hit
GCGTCCGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACAGAGAAG5320.1631481458765226No Hit
GCGTCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5100.15640141803952354No Hit
GCGTCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC5060.15517474025097827No Hit
GCGTCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA4870.14934802075538817No Hit
GCGTCCGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG4810.14750800407257028No Hit
GCGTCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4810.14750800407257028No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT4770.14628132628402496No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCCAA4490.137694581764208No Hit
GCGTCCGGGATAGTAATCATTAGTAGACTGTTGCCTTGTGGGGTTCCTGTTGCTGAGA4110.12604114277302783No Hit
GCGTCCGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCG3970.12174777051311933No Hit
GCGTCCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGC3830.11745439825321083No Hit
GCGTCCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG3820.11714772880607452No Hit
GCGTCCGGGGTGCTGCGTCCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGC3670.11254768709902971No Hit
GCGTCCGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGC3570.10948099262766649No Hit
GCGTCCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3350.10273426479066744No Hit
GCGTCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA3300.10120091755498584No Hit
GCGTCCGGGAATCTTCATTTATCTGTGTCACTAATTCAAGAAGACTTTGGCCATAAAA3290.1008942481078495No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAT150.00717349652.07685552
TTACGAA150.00717349652.07685552
CGATCCA150.007177857752.0688550
GATCGCT150.00718222152.0608544
TAAGCGT150.00718222152.0608548
TGATCGT150.00719532452.03686537
GACGCTT150.00719532452.03686539
GCGAAAT150.007208444652.01289735
TAGCGTA150.007208444652.01289735
CATATGC353.864261E-852.01289433
TCCGTAG150.007217201451.99693328
CGCTGTA150.00722158351.98895627
AACGGTA203.4476107E-451.98895625
ACGCTGT150.00722158351.98895626
TAGCGCG150.007230351751.97300322
ACGCTAC150.007230351751.97300322
CTAGCGC251.6576489E-551.97323
TCCGATA150.00723473951.9650313
CGTGCAA251.6591548E-551.9650319
TGGGACG150.00723473951.9650316
Produced by FastQC (version 0.11.5)