FastQCFastQC Report
Tue 31 May 2016
SRR212810_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212810_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329291
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21270.6459332323082015No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA19280.5855003629008991No Hit
GCGTCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG16220.49257343808364035No Hit
GCGTCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14020.42576323069868294No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA11050.33556945072899047No Hit
GCGTCCGGGATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGCTT10950.3325326231205833No Hit
GCGTCCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT9460.2872838917553167No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8180.24841249836770515No Hit
GCGTCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA7970.24203516039005016No Hit
GCGTCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA7620.23140626376062512No Hit
GCGTCCGGGAGTTCAAGTTTAGAGCTAAATTCTTTCCTGAAGATGTTTCTGAGGAATT6700.2034674497632793No Hit
GCGTCCGGGAGCAGTGAGTTTTTTGTAACCAGTCTCTTGTCTCAGGCTCTCTGCCTTG6430.19526801522057996No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA5660.1718844426358449No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA5560.16884761502743773No Hit
GCGTCCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5490.16672183570155272No Hit
GCGTCCGGGAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCA5480.16641815294071202No Hit
GCGTCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5470.1661144701798713No Hit
GCGTCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC5310.16125554600641984No Hit
GCGTCCGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACAGAGAAG5050.15335979422456125No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT5020.1524487459420391No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCCAA4650.14121248379093265No Hit
GCGTCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4470.13574619409579977No Hit
GCGTCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA4360.1324056837265519No Hit
GCGTCCGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG4330.13149463544402973No Hit
GCGTCCGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCG4090.12420624918385258No Hit
GCGTCCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG3740.11357735255442755No Hit
GCGTCCGGGATAGTAATCATTAGTAGACTGTTGCCTTGTGGGGTTCCTGTTGCTGAGA3560.10811106285929467No Hit
GCGTCCGGGGTGCTGCGTCCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGC3500.10628896629425036No Hit
GCGTCCGGGATCTATGATATGTGTTTCTTTTTTCCTGTCTGACTTCCTACTCAGTCAT3310.10051899383827678No Hit
GCGTCCGGGATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAAAGAGTTT3300.10021531107743606No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTG150.00715761852.10615524
TCGGTAG150.00715761852.10615526
CGATTGT150.007166244652.09028623
ACGCTAC150.007166244652.09028622
GTCGGCT150.007192170252.04274449
GCGACGC150.00720082852.02691746
CTATCCG150.00720082852.02691746
GTCGCAT150.007222505351.9873941
GGTCGCA150.007222505351.9873940
GCAACTA150.007222505351.9873933
TCATAGG150.007222505351.9873928
TCGACCC150.007222505351.9873933
GGTTACG353.884088E-851.97949234
ACCCACG150.007226846651.97949235
GCGCGAC150.007239882451.955819
GGATCGA251.6609347E-551.955818
CGCGACC150.007239882451.9558110
GGGCGAT150.007239882451.955817
TATCGAT150.007239882451.9558120
CGGGCGA650.051.9558076
Produced by FastQC (version 0.11.5)