FastQCFastQC Report
Tue 31 May 2016
SRR212809_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212809_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330932
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19270.5822948521146338No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA18690.5647685929435655No Hit
GCGTCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG14020.423651988928239No Hit
GCGTCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12610.3810450485296073No Hit
GCGTCCGGGATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGCTT10070.30429212043561815No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA9340.2822332080306528No Hit
GCGTCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA8140.24597198215947688No Hit
GCGTCCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT8130.24566980527721705No Hit
GCGTCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA7190.21726517834479592No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6930.2094085794060411No Hit
GCGTCCGGGAGCAGTGAGTTTTTTGTAACCAGTCTCTTGTCTCAGGCTCTCTGCCTTG5910.17858653741554156No Hit
GCGTCCGGGAGTTCAAGTTTAGAGCTAAATTCTTTCCTGAAGATGTTTCTGAGGAATT5720.17284517665260538No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA5240.15834068630413498No Hit
GCGTCCGGGAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCA5220.1577363325396154No Hit
GCGTCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC5080.1535058561879782No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA5060.1529015024234586No Hit
GCGTCCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4890.147764495425042No Hit
GCGTCCGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACAGAGAAG4760.1438361959556646No Hit
GCGTCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4630.1399078964862872No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCCAA4420.13356218195883143No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT4220.1275186443136354No Hit
GCGTCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA4050.12238163731521884No Hit
GCGTCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3920.11845333784584143No Hit
GCGTCCGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG3760.11361850772968465No Hit
GCGTCCGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCG3520.10636626255544947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGTG251.5890724E-552.3454869
GGCCGGT203.33327E-452.345489
CGGGATA10050.052.085066
CCGTAAA307.914841E-752.05961638
TCTGTCG307.973031E-752.0043951
TCATCGA150.007221839451.98863634
CGAGACT150.007234828551.9650240
CTAGGAT308.0231985E-751.95715345
CGAATTG150.007252174451.93356323
CCGACAA150.007252174451.93356323
CGGTACA251.6756316E-551.8786121
AACGTCG150.00728260451.87860516
GCGAATT150.00728260451.87860522
ACGTCGG150.00728260451.87860517
CACTCGT203.4866363E-451.8707715
GACGTAC150.00728695951.87076619
TGACCGG150.00728695951.87076613
CCCCGAT150.007291315651.86292618
CCGGGAT64600.051.6567275
CGGGATT22900.051.545456
Produced by FastQC (version 0.11.5)