FastQCFastQC Report
Tue 31 May 2016
SRR212808_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212808_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351387
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC30000.8537595300907546No Hit
GCGTCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC21190.603038814754103No Hit
GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19920.566896327980261No Hit
GCGTCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12900.36711659793902446No Hit
GCGTCCGGGATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGC10190.2899936537208263No Hit
GCGTCCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG7810.22226206433362647No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7510.2137244690327189No Hit
GCGTCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG7240.2060406332619021No Hit
GCGTCCGGGAGCAGTGAGTTTTTTGTAACCAGTCTCTTGTCTCAGGCTCTCTGCCT6130.17445153064854418No Hit
GCGTCCGGGAGTTCAAGTTTAGAGCTAAATTCTTTCCTGAAGATGTTTCTGAGGAA5560.15823009957681985No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT5480.15595340749657785No Hit
GCGTCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT5470.1556688209865476No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT5290.15054626380600308No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC5190.14770039870570056No Hit
GCGTCCGGGATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAAAGAGT5040.14343160105524677No Hit
GCGTCCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4970.14143949548503504No Hit
GCGTCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA4880.13887821689476273No Hit
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4850.138024457364672No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC4320.12294137233306868No Hit
GCGTCCGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTC4300.12237219931300818No Hit
GCGTCCGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA4180.11895716119264516No Hit
GCGTCCGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACAGAGA4140.11781881515252415No Hit
GCGTCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4100.11668046911240312No Hit
GCGTCCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3990.11355001750207036No Hit
GCGTCCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC3980.1132654309920401No Hit
GCGTCCGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC3800.10814287381149558No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC3680.10472783569113256No Hit
GCGTCCGGGATCTTTTACACCGTTGCTTTTATTTTTATAAATTATTTTCTCGTTGC3530.1004590380406788No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCC204.076956E-450.25322750
CGATTGA150.00826890250.23880848
TCGTTAC204.1292116E-450.1237134
GGTTACG252.0542182E-550.1237134
CTCGTTA204.1321304E-450.11653533
AGTGCGA150.00839154550.05204830
ATGCGTC150.00839154550.05204830
CGCCTAA150.00840578350.03058629
TCTATCG150.00840578350.03058629
CCGCCTA150.00841053350.02343828
ATGCCGC150.00843431249.98772425
TCGAACA150.00846291349.9449323
ACGACGT150.00846291349.9449323
TACGACG150.00847246349.93068322
CAACGCG204.2085917E-449.9306822
ACGGGTT150.0085251349.85246719
TCGGATA150.0085251349.85246719
CAGTACG150.0085251349.85246719
CGGGTTA507.2759576E-1249.8169946
GACCGTT150.0085491549.8169949
Produced by FastQC (version 0.11.5)