FastQCFastQC Report
Tue 31 May 2016
SRR212807_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212807_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332130
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC27930.8409357781591545No Hit
GCGTCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC21400.6443260169210852No Hit
GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18100.5449673320687682No Hit
GCGTCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12790.3850901755336766No Hit
GCGTCCGGGATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGC8710.26224671062535754No Hit
GCGTCCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG7380.2222021497606359No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7080.2131695420467889No Hit
GCGTCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG6270.18878150121940204No Hit
GCGTCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT5850.17613585042001625No Hit
GCGTCCGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT5020.15114563574503959No Hit
GCGTCCGGGAGCAGTGAGTTTTTTGTAACCAGTCTCTTGTCTCAGGCTCTCTGCCT5020.15114563574503959No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT4980.14994128804985998No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC4920.1481347665070906No Hit
GCGTCCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4890.1472315057357059No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT4840.1457260711167314No Hit
GCGTCCGGGAGTTCAAGTTTAGAGCTAAATTCTTTCCTGAAGATGTTTCTGAGGAA4700.14151085418360282No Hit
GCGTCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA4700.14151085418360282No Hit
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4630.1394032457170385No Hit
GCGTCCGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACAGAGA4390.1321771595459609No Hit
GCGTCCGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTC4340.13067172492698642No Hit
GCGTCCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC4240.12766085568903743No Hit
GCGTCCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4020.12103694336554963No Hit
GCGTCCGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC3920.11802607412760063No Hit
GCGTCCGGGATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAAAGAGT3900.11742390028001083No Hit
GCGTCCGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA3850.11591846566103635No Hit
GCGTCCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC3790.11411194411826694No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC3760.11320868334688224No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC3660.11019781410893324No Hit
GCGTCCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC3630.10929455333754856No Hit
GCGTCCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3590.10809020564236896No Hit
GCGTCCGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG3500.10538042332821486No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCC150.00827742450.22510542
CGTCCGA150.00827742450.22510543
CGGTTAT150.00828239250.21748439
AACGGGC252.031381E-550.21748440
TAGCTCG150.00830726650.17940537
CGGTAAC150.0083172350.16419236
ACCGATT204.1217028E-450.14138435
TGACGCT150.00833219550.14138435
ACCGGTA150.00833718850.1337934
GACCGAT204.124786E-450.1337934
CGTCGTC150.00837219850.08067332
CGTGACC150.00838222150.06551430
CACCGAA150.00838222150.06551431
CTATTGG150.008412342550.02010328
CAATGCG204.1868398E-449.98232725
TAGACGA150.00850319349.88436522
CCACACG252.1167447E-549.8693320
ACGCCGT150.00854380449.82427219
GATACTT301.0724161E-649.8167729
GGTCGAC204.2559535E-449.8167729
Produced by FastQC (version 0.11.5)