FastQCFastQC Report
Tue 31 May 2016
SRR212806_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212806_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65475
Sequences flagged as poor quality0
Sequence length58
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15532.3718976708667427No Hit
CGTGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8691.3272241313478426No Hit
CGTGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5610.8568155784650631No Hit
CGTGGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4000.6109201985490645No Hit
CGTGGCGGGGTGTTACAAAAATAAGAATTGGAGCATTAACTTGAAATTGTCTTCAAGG3930.6002290950744559No Hit
CGTGGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3040.4642993508972891No Hit
CGTGGCGGGATTTAATTTTGACTAAAATGTGTCATTTGTATAAATTGTAACATCCGAT2890.4413898434516991No Hit
CGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2890.4413898434516991No Hit
CGTGGCGGGAGCTAGTGAGATGATATTTGTCTTTCTGAAAGACTGGTTTAATTTGCTT2850.4352806414662085No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCTGA2330.3558610156548301No Hit
CGTGGCGGGATAGAACAATTGCTTGGTTCTTGCCTTCAGTAGAATGGTTTTTTGTTTT2180.3329515082092402No Hit
CGTGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2070.3161512027491409No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCAA2010.30698739977090495No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA1620.24742268041237112No Hit
CGTGGCGGGAAGTAAGAATATTAAAATAGCAAGGAATGATGATTAAGGCTGAAGTGTA1480.2260404734631539No Hit
CGTGGCGGGAGTTACCATGGATTTTTCCAAATCTCTTTTGGTTTTTGTTTAACAAATG1340.20465826651393662No Hit
CGTGGCGGGGATTAAGAAGAAAGAGTTTAAGTGTCAATAAACTTAAAACTACTGTTGT1100.16800305460099274No Hit
CGTGGCGGGGTGTTGGGTGCGTTGATTTAGATACGTTTATGTTCTCTGTTTAGGCCCA1090.1664757541046201No Hit
CGTGGCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA970.14814814814814814No Hit
CGTGGCGGGAACTAGAGATGTTCATGGTCAAAGTACTGCCTTTGCAGAGGAGCCTGTT970.14814814814814814No Hit
CGTGGCGGGGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGGAT960.1466208476517755No Hit
CGTGGCGGGATTTCAGTCATCTTCAGTTCTGCTGATGAGTTGTTGGAGTCTCTGTTGT950.14509354715540282No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA900.13745704467353953No Hit
CGTGGCGGGAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGCTTTG900.13745704467353953No Hit
CGTGGCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA860.13134784268804886No Hit
CGTGGCGGGATACAAATATTACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGAT830.1267659411989309No Hit
CGTGGCGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAAG770.11760213822069492No Hit
CGTGGCGGGCAGCAAAGAGGACGACCCTAAATGGTCGATGAAGAGTTTGATTTTATTT740.11302023673157695No Hit
CGTGGCGGGACACAGGAAGAAAGCAGGGCTGGCACAAGAGCTTCTGCAGAGAAAGAGG740.11302023673157695No Hit
CGTGGCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG740.11302023673157695No Hit
CGTGGCGGGATTTCAAGTAAGAATATTAAAATAGCAAGGAATGATGATTAAGGCTGAA710.10843833524245895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACA150.00718991452.036
GTGTTAC458.913048E-1152.010
CATGGAC150.00718991452.032
CGTGGAA150.00718991452.035
TTAGGAA150.00718991452.013
AGACTAC150.00718991452.051
TAGAGCG150.00718991452.047
GATACAA150.00718991452.09
GAAGCAT307.878916E-752.09
GATGTTA150.00718991452.09
GGTGTTA458.913048E-1152.09
ATTAGTC150.00718991452.050
ATTAGGA150.00718991452.012
TTCAGTA251.6379623E-552.035
ATTAGAA150.00718991452.020
AAGAGTG150.00718991452.018
GAGCTAG600.052.09
GAGCTAA150.00718991452.09
GAATGGT307.878916E-752.042
ACTTGAA503.6379788E-1252.039
Produced by FastQC (version 0.11.5)