SRR212805_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212805_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	63672
Sequences flagged as poor quality	0
Sequence length	58
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1537	2.413933911295389	No Hit
CGTGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	919	1.4433345897725844	No Hit
CGTGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	568	0.8920718683251665	No Hit
CGTGGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	402	0.6313607237090086	No Hit
CGTGGCGGGGTGTTACAAAAATAAGAATTGGAGCATTAACTTGAAATTGTCTTCAAGG	353	0.5544038195753235	No Hit
CGTGGCGGGATTTAATTTTGACTAAAATGTGTCATTTGTATAAATTGTAACATCCGAT	282	0.4428948360346777	No Hit
CGTGGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	281	0.44132428697072495	No Hit
CGTGGCGGGAGCTAGTGAGATGATATTTGTCTTTCTGAAAGACTGGTTTAATTTGCTT	258	0.40520165849981155	No Hit
CGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	253	0.3973489131800478	No Hit
CGTGGCGGGATAGAACAATTGCTTGGTTCTTGCCTTCAGTAGAATGGTTTTTTGTTTT	232	0.36436738283703984	No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCAA	202	0.3172509109184571	No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCTGA	194	0.304686518406835	No Hit
CGTGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	187	0.29369267495916573	No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA	164	0.25757004648825227	No Hit
CGTGGCGGGAAGTAAGAATATTAAAATAGCAAGGAATGATGATTAAGGCTGAAGTGTA	148	0.23244126146500818	No Hit
CGTGGCGGGGTGTTGGGTGCGTTGATTTAGATACGTTTATGTTCTCTGTTTAGGCCCA	112	0.1759014951627089	No Hit
CGTGGCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	111	0.17433094609875613	No Hit
CGTGGCGGGGATTAAGAAGAAAGAGTTTAAGTGTCAATAAACTTAAAACTACTGTTGT	111	0.17433094609875613	No Hit
CGTGGCGGGAGTTACCATGGATTTTTCCAAATCTCTTTTGGTTTTTGTTTAACAAATG	103	0.16176655358713407	No Hit
CGTGGCGGGACACAGGAAGAAAGCAGGGCTGGCACAAGAGCTTCTGCAGAGAAAGAGG	91	0.14291996481970096	No Hit
CGTGGCGGGGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGGAT	82	0.12878502324412613	No Hit
CGTGGCGGGAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGCTTTG	79	0.12407337605226787	No Hit
CGTGGCGGGCAGCAAAGAGGACGACCCTAAATGGTCGATGAAGAGTTTGATTTTATTT	77	0.12093227792436236	No Hit
CGTGGCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG	76	0.1193617288604096	No Hit
CGTGGCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	74	0.11622063073250409	No Hit
CGTGGCGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAAG	72	0.11307953260459858	No Hit
CGTGGCGGGAACTAGAGATGTTCATGGTCAAAGTACTGCCTTTGCAGAGGAGCCTGTT	70	0.10993843447669306	No Hit
CGTGGCGGGATCCAGGCATGTGTTCCTCCTCTTCCTTGCTCATGTCTGACCTCCAGCT	69	0.1083678854127403	No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA	68	0.10679733634878753	No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGGTAGAGCACCCGACTGCTCTTCCGA	66	0.10365623822088202	No Hit
CGTGGCGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	64	0.1005151400929765	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTAAC	35	3.7805876E-8	52.044064	45
ATCCGAT	35	3.7805876E-8	52.044064	52
TAAATTG	35	3.7805876E-8	52.044064	41
ACATCCG	35	3.7805876E-8	52.044064	50
CATCCGA	35	3.7805876E-8	52.044064	51
TGTAACA	35	3.7805876E-8	52.044064	46
GTAACAT	35	3.7805876E-8	52.044064	47
TGTTTAT	20	3.4086322E-4	52.04406	41
CTATGCG	15	0.007164921	52.04406	50
ATCTCCC	15	0.007164921	52.04406	42
GAATGGT	25	1.629231E-5	52.04406	42
GTTTAAC	15	0.007164921	52.04406	47
AACTTGA	55	0.0	52.04406	38
CGGTTCC	15	0.007164921	52.04406	48
GCTATGG	25	1.629231E-5	52.04406	50
CGCGCCG	15	0.007164921	52.04406	52
AATGGTT	25	1.629231E-5	52.04406	43
TTATCCG	20	3.4086322E-4	52.04406	44
AGCGGTT	15	0.007164921	52.04406	27
ATCCGAG	20	3.4086322E-4	52.04406	46

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content