FastQCFastQC Report
Tue 31 May 2016
SRR212804_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212804_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67218
Sequences flagged as poor quality0
Sequence length58
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14082.094677021036032No Hit
CGTGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8111.2065220625427713No Hit
CGTGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4800.7140944389895564No Hit
CGTGGCGGGGTGTTACAAAAATAAGAATTGGAGCATTAACTTGAAATTGTCTTCAAGG3610.5370585259900622No Hit
CGTGGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3600.5355708292421673No Hit
CGTGGCGGGATTTAATTTTGACTAAAATGTGTCATTTGTATAAATTGTAACATCCGAT2810.41804278615846946No Hit
CGTGGCGGGATAGAACAATTGCTTGGTTCTTGCCTTCAGTAGAATGGTTTTTTGTTTT2470.36746109673004257No Hit
CGTGGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2420.36002261299056804No Hit
CGTGGCGGGAGCTAGTGAGATGATATTTGTCTTTCTGAAAGACTGGTTTAATTTGCTT2320.3451456455116189No Hit
CGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.3451456455116189No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCAA2010.2990270463268767No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCTGA1910.28415007884792765No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA1510.22464220893213127No Hit
CGTGGCGGGAAGTAAGAATATTAAAATAGCAAGGAATGATGATTAAGGCTGAAGTGTA1470.21869142194055163No Hit
CGTGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.21274063494897197No Hit
CGTGGCGGGGTGTTGGGTGCGTTGATTTAGATACGTTTATGTTCTCTGTTTAGGCCCA1220.1814990032431789No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA1110.1651343390163349No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1000.1487696747894909No Hit
CGTGGCGGGGATTAAGAAGAAAGAGTTTAAGTGTCAATAAACTTAAAACTACTGTTGT1000.1487696747894909No Hit
CGTGGCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA970.14430658454580617No Hit
CGTGGCGGGAGTTACCATGGATTTTTCCAAATCTCTTTTGGTTTTTGTTTAACAAATG920.13686810080633163No Hit
CGTGGCGGGGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGGAT880.130917313814752No Hit
CGTGGCGGGATTTCAGTCATCTTCAGTTCTGCTGATGAGTTGTTGGAGTCTCTGTTGT870.1294296170668571No Hit
CGTGGCGGGAACTAGAGATGTTCATGGTCAAAGTACTGCCTTTGCAGAGGAGCCTGTT840.12496652682317236No Hit
CGTGGCGGGACACAGGAAGAAAGCAGGGCTGGCACAAGAGCTTCTGCAGAGAAAGAGG830.12347883007527746No Hit
CGTGGCGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAAG810.12050343657948764No Hit
CGTGGCGGGAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGCTTTG780.11604034633580292No Hit
CGTGGCGGGATACAAATATTACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGAT710.10562646910053854No Hit
CGTGGCGGGATCCAGGCATGTGTTCCTCCTCTTCCTTGCTCATGTCTGACCTCCAGCT700.10413877235264364No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACCA150.00706896952.2247239
AGAAGCG203.3520468E-452.22471638
GACAGTA150.007110984552.146641
AGCGTGG150.007110984552.146643
GGGTGCC203.3769468E-452.14668
ATCTCAG150.007110984552.146610
GGAGCGA150.007110984552.14668
GGATGTC203.3769468E-452.14668
TTAGCTT150.007110984552.146643
GGGTCTA251.611273E-552.14668
GGGTATG150.007110984552.14668
GAACTCA150.007110984552.14669
GATCCAT150.007110984552.14669
GATCCAG251.611273E-552.14669
GGATCTA203.3769468E-452.14668
ATGCTAT150.007110984552.146610
GGATATA150.007110984552.14668
GGATAGA203.3769468E-452.14668
CGGGGTC2600.052.14666
GGGGTAT251.611273E-552.14667
Produced by FastQC (version 0.11.5)