FastQCFastQC Report
Tue 31 May 2016
SRR212803_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212803_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90492
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14331.5835653980462363No Hit
CGTGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9421.0409759978782656No Hit
CGTGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4800.5304336294921098No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4270.4718649162356894No Hit
CGTGGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3930.4342925341466649No Hit
CGTGGCGGGGTGTTACAAAAATAAGAATTGGAGCATTAACTTGAAATTGTCTTCAA3360.37130354064447685No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT3200.35362241966140656No Hit
CGTGGCGGGATTTAATTTTGACTAAAATGTGTCATTTGTATAAATTGTAACATCCG3140.34699199929275515No Hit
CGTGGCGGGATAGAACAATTGCTTGGTTCTTGCCTTCAGTAGAATGGTTTTTTGTT2790.30831454714228884No Hit
CGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2730.30168412677363743No Hit
CGTGGCGGGAGCTAGTGAGATGATATTTGTCTTTCTGAAAGACTGGTTTAATTTGC2480.2740573752375901No Hit
CGTGGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2390.264111744684613No Hit
CGTGGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC2350.2596914644388454No Hit
CGTGGCGGGAGTTACCATGGATTTTTCCAAATCTCTTTTGGTTTTTGTTTAACAAA2040.22543429253414665No Hit
CGTGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.16907571940061No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCC1500.1657605092162843No Hit
CGTGGCGGGGTGTTGGGTGCGTTGATTTAGATACGTTTATGTTCTCTGTTTAGGCC1370.1513945984175397No Hit
CGTGGCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1360.1502895283560978No Hit
CGTGGCGGGATTTCAGTCATCTTCAGTTCTGCTGATGAGTTGTTGGAGTCTCTGTT1320.1458692481103302No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC1220.13481854749591124No Hit
CGTGGCGGGAAGTAAGAATATTAAAATAGCAAGGAATGATGATTAAGGCTGAAGTG1180.13039826725014367No Hit
CGTGGCGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCA1100.1215577067586085No Hit
CGTGGCGGGAACTAGAGATGTTCATGGTCAAAGTACTGCCTTTGCAGAGGAGCCTG1080.1193475666357247No Hit
CGTGGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGGGCGGGGCCTTCTC1050.11603235645139902No Hit
CGTGGCGGGGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGG1000.11050700614418955No Hit
CGTGGCGGGGATTAAGAAGAAAGAGTTTAAGTGTCAATAAACTTAAAACTACTGTT980.10829686602130575No Hit
CGTGGCGGGGAAGTACCCCATTGAACATGGCATTGTTACCAACTGGGACGACATGG960.10608672589842195No Hit
CGTGGCGGGAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGCTT950.10498165583698006No Hit
CGTGGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGGGCGCTT930.10277151571409628No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCATT150.00822162250.28109748
TATCTCA150.00822162250.28109748
GGTGGCG150.00822162250.28109748
TAGATCC150.00822162250.28109750
ACATCCG204.0495495E-450.28109450
GCGAGCC204.0495495E-450.28109448
GTCGCAT150.00825795850.22507544
CAATTAT150.00825795850.22507544
TTATCTC150.00825795850.22507547
TCTCGTG150.00825795850.22507546
TGTTTAG252.0176632E-550.2250747
AGCAACC204.0719102E-450.2250746
CACCTAT204.0719102E-450.2250745
ACCTATG204.0719102E-450.2250746
TGACACT204.0719102E-450.2250746
GAGTCGG301.0092645E-650.19710540
TCACAAT150.00827617150.19710541
GAAAGTT204.083127E-450.19710542
GGTTGTT150.00827617150.19710541
TAAATTG204.083127E-450.19710541
Produced by FastQC (version 0.11.5)