SRR212802_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212802_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	89636
Sequences flagged as poor quality	0
Sequence length	56
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1280	1.4279976795037708	No Hit
CGTGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	889	0.9917890133428533	No Hit
CGTGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	440	0.4908742023294212	No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	419	0.46744611540006253	No Hit
CGTGGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	389	0.4339774197866929	No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT	345	0.38488999955375075	No Hit
CGTGGCGGGGTGTTACAAAAATAAGAATTGGAGCATTAACTTGAAATTGTCTTCAA	330	0.3681556517470659	No Hit
CGTGGCGGGATTTAATTTTGACTAAAATGTGTCATTTGTATAAATTGTAACATCCG	282	0.3146057387656745	No Hit
CGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	271	0.30233388370743897	No Hit
CGTGGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	256	0.28559953590075415	No Hit
CGTGGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	225	0.25101521710027225	No Hit
CGTGGCGGGATAGAACAATTGCTTGGTTCTTGCCTTCAGTAGAATGGTTTTTTGTT	216	0.24097460841626134	No Hit
CGTGGCGGGAGCTAGTGAGATGATATTTGTCTTTCTGAAAGACTGGTTTAATTTGC	210	0.2342808692935874	No Hit
CGTGGCGGGAGTTACCATGGATTTTTCCAAATCTCTTTTGGTTTTTGTTTAACAAA	182	0.20304342005444243	No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCC	169	0.18854031862198226	No Hit
CGTGGCGGGGTGTTGGGTGCGTTGATTTAGATACGTTTATGTTCTCTGTTTAGGCC	145	0.16176536213128653	No Hit
CGTGGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGGGCGGGGCCTTCTC	143	0.1595341157570619	No Hit
CGTGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	133	0.1483778838859387	No Hit
CGTGGCGGGATTTCAGTCATCTTCAGTTCTGCTGATGAGTTGTTGGAGTCTCTGTT	132	0.14726226069882636	No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	132	0.14726226069882636	No Hit
CGTGGCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	115	0.1282966665179169	No Hit
CGTGGCGGGAAGTAAGAATATTAAAATAGCAAGGAATGATGATTAAGGCTGAAGTG	113	0.12606542014369226	No Hit
CGTGGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGGGCGCTT	105	0.1171404346467937	No Hit
CGTGGCGGGGTCTTTCAGTTTATCCTAACAGATGAGAGTCTTGCCTTTGTAGTTGA	101	0.11267794189834442	No Hit
CGTGGCGGGAACTAGAGATGTTCATGGTCAAAGTACTGCCTTTGCAGAGGAGCCTG	100	0.1115623187112321	No Hit
CGTGGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	100	0.1115623187112321	No Hit
CGTGGCGGGGAAGTACCCCATTGAACATGGCATTGTTACCAACTGGGACGACATGG	99	0.11044669552411977	No Hit
CGTGGCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	95	0.1059842027756705	No Hit
CGTGGCGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCA	94	0.10486857958855818	No Hit
CGTGGCGGGATCAACAAAACTTTCATCTTCTCTGATCTTGAGTATATGGGGCAGAG	93	0.10375295640144586	No Hit
CGTGGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTC	92	0.10263733321433353	No Hit
CGTGGCGGGATCCCAGTAAATCCATGTATATGCTCACTATATTTAATAACCACAAC	92	0.10263733321433353	No Hit
CGTGGCGGGGTCATCTTTGAGCTGCTTATCAAAATACAGATCATTATTGCATATAT	91	0.10152171002722121	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCAT	15	0.008168312	50.36343	49
AACCCAA	15	0.008168312	50.36343	49
ACAATAT	15	0.008168312	50.36343	49
ACTCAAT	15	0.008168312	50.36343	50
CGTTCGA	15	0.008186562	50.335022	48
TGGACTC	15	0.008204842	50.30665	47
AGCTTAC	15	0.008204842	50.30665	47
CCGTTCG	15	0.008204842	50.30665	47
ACTATTC	20	4.050434E-4	50.278313	45
CGACTGT	20	4.050434E-4	50.278313	44
TTAGCTT	15	0.008223153	50.27831	45
ACCGCCC	15	0.008223153	50.27831	46
TTCAAAG	15	0.008223153	50.27831	46
AATTATC	15	0.008223153	50.27831	45
ATTATCT	15	0.008223153	50.27831	46
GAAATTG	40	2.5211193E-9	50.25	43
TGTTATG	15	0.008241494	50.249996	43
GACTCGC	20	4.073027E-4	50.22172	41
TCTTTAG	15	0.008259865	50.221718	42
GAAAGAC	20	4.0957198E-4	50.165264	37

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content