FastQCFastQC Report
Tue 31 May 2016
SRR212801_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212801_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425957
Sequences flagged as poor quality0
Sequence length58
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28800.6761245853454692No Hit
AGTCGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21170.49699852332512434No Hit
AGTCGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT16020.3760943005984172No Hit
AGTCGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG15410.36177360625603056No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA15360.3605997788509169No Hit
AGTCGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT11900.27937092241705147No Hit
AGTCGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA11880.27890139145500603No Hit
AGTCGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC10930.2565986707578465No Hit
AGTCGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT10310.24204321093443704No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA9700.2277225165920504No Hit
AGTCGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG9530.2237315034146639No Hit
AGTCGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC9310.2185666628321638No Hit
AGTCGTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT8550.20072448627443618No Hit
AGTCGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAG8490.19931589338829975No Hit
AGTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7900.18546473000795857No Hit
AGTCGTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6840.16057958901954894No Hit
AGTCGTGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG6390.15001514237352598No Hit
AGTCGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA6350.14907608044943504No Hit
AGTCGTGGGCACCAAGGGATCTACAGAGTGAGCGAGTTTCCTGGAGAGGCCAGCGAGG6280.1474327220822759No Hit
AGTCGTGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6120.1436764743859122No Hit
AGTCGTGGGGTCACGGGATCGTGGGTTCGAGTCCCACCTCGTGTTTTGTTGCGAGATC5960.13992022668954845No Hit
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5700.13381632418295744No Hit
AGTCGTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT5550.13029484196761645No Hit
AGTCGTGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA5240.12301711205591176No Hit
AGTCGTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGC5170.12137375368875263No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA5090.11949562984057076No Hit
AGTCGTGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC4960.11644367858727524No Hit
AGTCGTGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG4880.11456555473909338No Hit
AGTCGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC4810.11292219637193425No Hit
AGTCGTGGGATTTGGAGGACATGATAGCTTGCATAAAAGATGACGCTATAGTTTATGC4750.11151360348579786No Hit
AGTCGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4740.11127883800477513No Hit
AGTCGTGGGATCGGTGTACTCCGAAAAGGGAGAGTCATCTGGCAAGAATGTCACTTTG4670.109635479637616No Hit
AGTCGTGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAG4640.10893118319454781No Hit
AGTCGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC4590.10775735578943414No Hit
AGTCGTGGGGTTGCGAGATCGTGGGTTCGAGTCCCACCTCGCGTCTGGTCACGGGATC4560.10705305934636594No Hit
AGTCGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA4470.10494017001716135No Hit
AGTCGTGGGAACTTCTAAAATGCATATATTGGGGCTTGGGGGGATGGGTGTTTTTTAA4360.10235774972591129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCG503.6379788E-1252.02655447
CTCGCAC251.6484362E-552.02655446
ATCGCAT203.4365253E-452.02655447
CGTAGCG307.9615893E-752.0204441
AACCGTA150.007209171552.0143337
ACGTCCA150.007209171552.0143340
TTCGCAG150.007209171552.0143340
GTTTCGC251.6530275E-552.00211331
CCGACCG150.00721587252.00211332
CGGCGTG150.00721922451.99600620
TGCGGTC150.00721922451.99600626
AGACGCA203.4505024E-451.983819
CCAATCG150.007225930751.98379519
CTAACCG150.007225930751.98379517
ATTACAC150.007229286251.97769511
TCAATAC150.007229286251.97769512
GACGCAT150.007229286251.9776959
CGTGGGA184200.051.314574
AGTCGTG431700.051.285381
GTCGTGG432550.051.1846052
Produced by FastQC (version 0.11.5)